The influence of pH on dissolved organic matter fluorescence in inland waters
International audience Fluorescence is an easily available analytical technique used to assess the optical characteristics of dissolvedorganic matter (DOM). Despite widespread use, there has been some confusion about how robustfluorescence spectroscopy is to differences in solution pH. Here we asses...
Published in: | Analytical Methods |
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Online Access: | https://hal.science/hal-03625863 https://hal.science/hal-03625863/document https://hal.science/hal-03625863/file/d1ay01702k.pdf https://doi.org/10.1039/D1AY01702K |
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ftccsdartic:oai:HAL:hal-03625863v1 2023-06-11T04:07:40+02:00 The influence of pH on dissolved organic matter fluorescence in inland waters Groeneveld, M. Catalán, N. Einarsdottir, K. Bravo, A. Kothawala, D. Uppsala University Laboratoire des Sciences du Climat et de l'Environnement Gif-sur-Yvette (LSCE) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) Centre d'Investigació i Desenvolupament Barcelona (CID-CSIC) Consejo Superior de Investigaciones Científicas Madrid (CSIC) European Project: 839709,CHROME - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions 2022 https://hal.science/hal-03625863 https://hal.science/hal-03625863/document https://hal.science/hal-03625863/file/d1ay01702k.pdf https://doi.org/10.1039/D1AY01702K en eng HAL CCSD Royal Society of Chemistry info:eu-repo/semantics/altIdentifier/doi/10.1039/D1AY01702K info:eu-repo/grantAgreement//839709/EU/Integrating Arctic organic matter reactivity into Earth system models/CHROME - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions hal-03625863 https://hal.science/hal-03625863 https://hal.science/hal-03625863/document https://hal.science/hal-03625863/file/d1ay01702k.pdf doi:10.1039/D1AY01702K http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1759-9660 Analytical Methods https://hal.science/hal-03625863 Analytical Methods, 2022, 14, pp.1351-1360. ⟨10.1039/D1AY01702K⟩ [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces environment info:eu-repo/semantics/article Journal articles 2022 ftccsdartic https://doi.org/10.1039/D1AY01702K 2023-05-06T23:48:18Z International audience Fluorescence is an easily available analytical technique used to assess the optical characteristics of dissolvedorganic matter (DOM). Despite widespread use, there has been some confusion about how robustfluorescence spectroscopy is to differences in solution pH. Here we assess fluorescence characteristicsof three natural water samples and one commercially available standard (Nordic Reservoir) by modifyingthe pH across a range from 3.5 to 9.0 at 0.5 pH increments. We used two statistical approaches toassess if fluorescence intensity shifted significantly across this pH range. We identified that humic-likeand protein-like fluorescence was largely stable within the pH range of 5.5 to 7.5, which represents 80%of Swedish lakes and streams. Likewise, we found that the three commonly used fluorescence indiceswere robust across the full pH range tested with the exception of the humification index, which hada narrower range of stability. The commerical humic substance sample was highly unstable with changesto pH in the regions of protein-like fluorescence being particularly sensitive. One of our conclusions isthat differences in fluorescence intensity in the pH range of 5.5 to 7.5, typical for most inland waters, aregenerally minor. We recommend adjusting the pH when samples fall outside this region and to beespecially careful in interpreting results from commercial humic substances. Article in Journal/Newspaper Arctic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Analytical Methods 14 13 1351 1360 |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces environment |
spellingShingle |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces environment Groeneveld, M. Catalán, N. Einarsdottir, K. Bravo, A. Kothawala, D. The influence of pH on dissolved organic matter fluorescence in inland waters |
topic_facet |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces environment |
description |
International audience Fluorescence is an easily available analytical technique used to assess the optical characteristics of dissolvedorganic matter (DOM). Despite widespread use, there has been some confusion about how robustfluorescence spectroscopy is to differences in solution pH. Here we assess fluorescence characteristicsof three natural water samples and one commercially available standard (Nordic Reservoir) by modifyingthe pH across a range from 3.5 to 9.0 at 0.5 pH increments. We used two statistical approaches toassess if fluorescence intensity shifted significantly across this pH range. We identified that humic-likeand protein-like fluorescence was largely stable within the pH range of 5.5 to 7.5, which represents 80%of Swedish lakes and streams. Likewise, we found that the three commonly used fluorescence indiceswere robust across the full pH range tested with the exception of the humification index, which hada narrower range of stability. The commerical humic substance sample was highly unstable with changesto pH in the regions of protein-like fluorescence being particularly sensitive. One of our conclusions isthat differences in fluorescence intensity in the pH range of 5.5 to 7.5, typical for most inland waters, aregenerally minor. We recommend adjusting the pH when samples fall outside this region and to beespecially careful in interpreting results from commercial humic substances. |
author2 |
Uppsala University Laboratoire des Sciences du Climat et de l'Environnement Gif-sur-Yvette (LSCE) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) Centre d'Investigació i Desenvolupament Barcelona (CID-CSIC) Consejo Superior de Investigaciones Científicas Madrid (CSIC) European Project: 839709,CHROME - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions |
format |
Article in Journal/Newspaper |
author |
Groeneveld, M. Catalán, N. Einarsdottir, K. Bravo, A. Kothawala, D. |
author_facet |
Groeneveld, M. Catalán, N. Einarsdottir, K. Bravo, A. Kothawala, D. |
author_sort |
Groeneveld, M. |
title |
The influence of pH on dissolved organic matter fluorescence in inland waters |
title_short |
The influence of pH on dissolved organic matter fluorescence in inland waters |
title_full |
The influence of pH on dissolved organic matter fluorescence in inland waters |
title_fullStr |
The influence of pH on dissolved organic matter fluorescence in inland waters |
title_full_unstemmed |
The influence of pH on dissolved organic matter fluorescence in inland waters |
title_sort |
influence of ph on dissolved organic matter fluorescence in inland waters |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal.science/hal-03625863 https://hal.science/hal-03625863/document https://hal.science/hal-03625863/file/d1ay01702k.pdf https://doi.org/10.1039/D1AY01702K |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
ISSN: 1759-9660 Analytical Methods https://hal.science/hal-03625863 Analytical Methods, 2022, 14, pp.1351-1360. ⟨10.1039/D1AY01702K⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1039/D1AY01702K info:eu-repo/grantAgreement//839709/EU/Integrating Arctic organic matter reactivity into Earth system models/CHROME - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions hal-03625863 https://hal.science/hal-03625863 https://hal.science/hal-03625863/document https://hal.science/hal-03625863/file/d1ay01702k.pdf doi:10.1039/D1AY01702K |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1039/D1AY01702K |
container_title |
Analytical Methods |
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14 |
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13 |
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1351 |
op_container_end_page |
1360 |
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1768380831099781120 |