Tyrosine Hydroxylase in the European Eel (Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis
International audience We report the isolation of a full-length eel tyrosine hydroxylase (TH) cDNA that is characterized by a long 3' untranslated region and by a diversity restricted to the 3' end owing to the differential use of three polyadenylation signals. The longest eel TH mRNA was...
Published in: | Journal of Neurochemistry |
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Online Access: | https://hal.science/hal-03577618 https://doi.org/10.1046/j.1471-4159.1998.71020460.x |
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ftccsdartic:oai:HAL:hal-03577618v1 2024-01-14T09:59:20+01:00 Tyrosine Hydroxylase in the European Eel (Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis Boularand, Sylviane Biguet, Nicole Faucon Vidal, Bernadette Veron, Michèle Mallet, Jacques Vincent, Jean-Didier Dufour, Sylvie Vernier, Philippe Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs APHP Pitié-Salpêtrière (LGN) Université Pierre et Marie Curie - Paris 6 (UPMC)-CHU Pitié-Salpêtrière AP-HP Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie Générale et Comparée (LPGC) Centre National de la Recherche Scientifique (CNRS) Institut de Neurobiologie Alfred Fessard (INAF) 1998-08 https://hal.science/hal-03577618 https://doi.org/10.1046/j.1471-4159.1998.71020460.x en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1471-4159.1998.71020460.x info:eu-repo/semantics/altIdentifier/pmid/9681435 hal-03577618 https://hal.science/hal-03577618 doi:10.1046/j.1471-4159.1998.71020460.x PUBMED: 9681435 ISSN: 0022-3042 EISSN: 1471-4159 Journal of Neurochemistry https://hal.science/hal-03577618 Journal of Neurochemistry, 1998, 71 (2), pp.460-470. ⟨10.1046/j.1471-4159.1998.71020460.x⟩ MESH: Amino Acid Sequence MESH: Anguilla MESH: Evolution Molecular MESH: Gene Expression Regulation Enzymologic MESH: Genetic Variation MESH: In Situ Hybridization MESH: Molecular Sequence Data MESH: Phenylalanine Hydroxylase MESH: Phylogeny MESH: RNA Messenger MESH: Regulatory Sequences Nucleic Acid MESH: Tryptophan Hydroxylase MESH: Animals MESH: Tyrosine 3-Monooxygenase MESH: Antisense Elements (Genetics) MESH: Base Sequence MESH: Brain MESH: Brain Chemistry MESH: Catecholamines MESH: Cloning MESH: DNA Complementary [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences info:eu-repo/semantics/article Journal articles 1998 ftccsdartic https://doi.org/10.1046/j.1471-4159.1998.71020460.x 2023-12-17T00:23:23Z International audience We report the isolation of a full-length eel tyrosine hydroxylase (TH) cDNA that is characterized by a long 3' untranslated region and by a diversity restricted to the 3' end owing to the differential use of three polyadenylation signals. The longest eel TH mRNA was distinctive in the presence of four pentameric elements (AUUUA) in the AU-rich 3' noncoding region. Such a diversity could provide the basis of posttranscriptional or translational regulation of eel TH gene expression. Comparison of the eel TH sequence with those of other aromatic amino acid hydroxylases (TH, tryptophan hydroxylase, and phenylalanine hydroxylase) and phylogenetic analysis confirmed that the N-terminal regulatory domain is highly divergent, contrasting with the conservation of the catalytic core of the enzyme. Molecular phylogenies including the available sequences of the three hydroxylase genes suggested that the duplication of their common ancestor occurred before the emergence of arthropods. The regional expression of the eel TH mRNA was studied by semiquantitative PCR, northern blots, and in situ hybridization and compared with the immunocytochemical localization of TH protein. The data showed that TH mRNA is mostly expressed in the olfactory and hypothalamic areas, whereas sparse TH-expressing cell bodies are present in the telencephalic region and brainstem. No labeling was detected in the mesencephalic area, in striking contrast with that found in amphibians and amniotes. Article in Journal/Newspaper Anguilla anguilla Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Journal of Neurochemistry 71 2 460 470 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
MESH: Amino Acid Sequence MESH: Anguilla MESH: Evolution Molecular MESH: Gene Expression Regulation Enzymologic MESH: Genetic Variation MESH: In Situ Hybridization MESH: Molecular Sequence Data MESH: Phenylalanine Hydroxylase MESH: Phylogeny MESH: RNA Messenger MESH: Regulatory Sequences Nucleic Acid MESH: Tryptophan Hydroxylase MESH: Animals MESH: Tyrosine 3-Monooxygenase MESH: Antisense Elements (Genetics) MESH: Base Sequence MESH: Brain MESH: Brain Chemistry MESH: Catecholamines MESH: Cloning MESH: DNA Complementary [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences |
spellingShingle |
MESH: Amino Acid Sequence MESH: Anguilla MESH: Evolution Molecular MESH: Gene Expression Regulation Enzymologic MESH: Genetic Variation MESH: In Situ Hybridization MESH: Molecular Sequence Data MESH: Phenylalanine Hydroxylase MESH: Phylogeny MESH: RNA Messenger MESH: Regulatory Sequences Nucleic Acid MESH: Tryptophan Hydroxylase MESH: Animals MESH: Tyrosine 3-Monooxygenase MESH: Antisense Elements (Genetics) MESH: Base Sequence MESH: Brain MESH: Brain Chemistry MESH: Catecholamines MESH: Cloning MESH: DNA Complementary [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences Boularand, Sylviane Biguet, Nicole Faucon Vidal, Bernadette Veron, Michèle Mallet, Jacques Vincent, Jean-Didier Dufour, Sylvie Vernier, Philippe Tyrosine Hydroxylase in the European Eel (Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
topic_facet |
MESH: Amino Acid Sequence MESH: Anguilla MESH: Evolution Molecular MESH: Gene Expression Regulation Enzymologic MESH: Genetic Variation MESH: In Situ Hybridization MESH: Molecular Sequence Data MESH: Phenylalanine Hydroxylase MESH: Phylogeny MESH: RNA Messenger MESH: Regulatory Sequences Nucleic Acid MESH: Tryptophan Hydroxylase MESH: Animals MESH: Tyrosine 3-Monooxygenase MESH: Antisense Elements (Genetics) MESH: Base Sequence MESH: Brain MESH: Brain Chemistry MESH: Catecholamines MESH: Cloning MESH: DNA Complementary [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences |
description |
International audience We report the isolation of a full-length eel tyrosine hydroxylase (TH) cDNA that is characterized by a long 3' untranslated region and by a diversity restricted to the 3' end owing to the differential use of three polyadenylation signals. The longest eel TH mRNA was distinctive in the presence of four pentameric elements (AUUUA) in the AU-rich 3' noncoding region. Such a diversity could provide the basis of posttranscriptional or translational regulation of eel TH gene expression. Comparison of the eel TH sequence with those of other aromatic amino acid hydroxylases (TH, tryptophan hydroxylase, and phenylalanine hydroxylase) and phylogenetic analysis confirmed that the N-terminal regulatory domain is highly divergent, contrasting with the conservation of the catalytic core of the enzyme. Molecular phylogenies including the available sequences of the three hydroxylase genes suggested that the duplication of their common ancestor occurred before the emergence of arthropods. The regional expression of the eel TH mRNA was studied by semiquantitative PCR, northern blots, and in situ hybridization and compared with the immunocytochemical localization of TH protein. The data showed that TH mRNA is mostly expressed in the olfactory and hypothalamic areas, whereas sparse TH-expressing cell bodies are present in the telencephalic region and brainstem. No labeling was detected in the mesencephalic area, in striking contrast with that found in amphibians and amniotes. |
author2 |
Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs APHP Pitié-Salpêtrière (LGN) Université Pierre et Marie Curie - Paris 6 (UPMC)-CHU Pitié-Salpêtrière AP-HP Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie Générale et Comparée (LPGC) Centre National de la Recherche Scientifique (CNRS) Institut de Neurobiologie Alfred Fessard (INAF) |
format |
Article in Journal/Newspaper |
author |
Boularand, Sylviane Biguet, Nicole Faucon Vidal, Bernadette Veron, Michèle Mallet, Jacques Vincent, Jean-Didier Dufour, Sylvie Vernier, Philippe |
author_facet |
Boularand, Sylviane Biguet, Nicole Faucon Vidal, Bernadette Veron, Michèle Mallet, Jacques Vincent, Jean-Didier Dufour, Sylvie Vernier, Philippe |
author_sort |
Boularand, Sylviane |
title |
Tyrosine Hydroxylase in the European Eel (Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_short |
Tyrosine Hydroxylase in the European Eel (Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_full |
Tyrosine Hydroxylase in the European Eel (Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_fullStr |
Tyrosine Hydroxylase in the European Eel (Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_full_unstemmed |
Tyrosine Hydroxylase in the European Eel (Anguilla anguilla): cDNA Cloning, Brain Distribution, and Phylogenetic Analysis |
title_sort |
tyrosine hydroxylase in the european eel (anguilla anguilla): cdna cloning, brain distribution, and phylogenetic analysis |
publisher |
HAL CCSD |
publishDate |
1998 |
url |
https://hal.science/hal-03577618 https://doi.org/10.1046/j.1471-4159.1998.71020460.x |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 0022-3042 EISSN: 1471-4159 Journal of Neurochemistry https://hal.science/hal-03577618 Journal of Neurochemistry, 1998, 71 (2), pp.460-470. ⟨10.1046/j.1471-4159.1998.71020460.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1471-4159.1998.71020460.x info:eu-repo/semantics/altIdentifier/pmid/9681435 hal-03577618 https://hal.science/hal-03577618 doi:10.1046/j.1471-4159.1998.71020460.x PUBMED: 9681435 |
op_doi |
https://doi.org/10.1046/j.1471-4159.1998.71020460.x |
container_title |
Journal of Neurochemistry |
container_volume |
71 |
container_issue |
2 |
container_start_page |
460 |
op_container_end_page |
470 |
_version_ |
1788058564917460992 |