Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker

International audience The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe helminthic zoonotic disease distributed in the Northern Hemisphere. The lifecycle of the parasite is mainly sylvatic, involving canid and rodent hosts. The absence of genetic da...

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Published in:Infection, Genetics and Evolution
Main Authors: Umhang, Gérald, Bastid, Vanessa, Avcioglu, Hamza, Bagrade, Guna, Bujanić, Miljenko, Bjelić Čabrilo, Oliveira, Casulli, Adriano, Dorny, Pierre, Van Der Giessen, Joke, Guven, Esin, Harna, Jiri, Karamon, Jacek, Kharchenko, Vitaliy, Knapp, Jenny, Kolarova, Libuse, Konyaev, Sergey, Laurimaa, Leidi, Losch, Serge, Miljević, Milan, Miterpakova, Martina, Moks, Epp, Romig, Thomas, Saarma, Urmas, Snabel, Viliam, Sreter, Tamas, Valdmann, Harri, Boué, Franck
Other Authors: Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2021
Subjects:
Online Access:https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264
https://doi.org/10.1016/j.meegid.2021.104863
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spelling ftccsdartic:oai:HAL:hal-03516264v1 2023-05-15T15:15:30+02:00 Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker Umhang, Gérald Bastid, Vanessa Avcioglu, Hamza Bagrade, Guna Bujanić, Miljenko Bjelić Čabrilo, Oliveira Casulli, Adriano Dorny, Pierre Van Der Giessen, Joke Guven, Esin Harna, Jiri Karamon, Jacek Kharchenko, Vitaliy Knapp, Jenny Kolarova, Libuse Konyaev, Sergey Laurimaa, Leidi Losch, Serge Miljević, Milan Miterpakova, Martina Moks, Epp Romig, Thomas Saarma, Urmas Snabel, Viliam Sreter, Tamas Valdmann, Harri Boué, Franck Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE) Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC) Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC) 2021-08 https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 https://doi.org/10.1016/j.meegid.2021.104863 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2021.104863 info:eu-repo/semantics/altIdentifier/pmid/33857665 hal-03516264 https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 doi:10.1016/j.meegid.2021.104863 PUBMED: 33857665 ISSN: 1567-1348 EISSN: 1567-7257 Infection, Genetics and Evolution https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 Infection, Genetics and Evolution, Elsevier, 2021, 92, pp.104863. ⟨10.1016/j.meegid.2021.104863⟩ Asia Echinococcus multilocularis EmsB microsatellite Europe Parasite expansion [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health info:eu-repo/semantics/article Journal articles 2021 ftccsdartic https://doi.org/10.1016/j.meegid.2021.104863 2022-01-08T23:22:40Z International audience The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe helminthic zoonotic disease distributed in the Northern Hemisphere. The lifecycle of the parasite is mainly sylvatic, involving canid and rodent hosts. The absence of genetic data from most eastern European countries is a major knowledge gap, affecting the study of associations with parasite populations in Western Europe. In this study, EmsB microsatellite genotyping of E. multilocularis was performed to describe the genetic diversity and relatedness of 785 E. multilocularis isolates from four western and nine eastern European countries, as well as from Armenia and the Asian parts of Russia and Turkey. The presence of the same E. multilocularis populations in the Benelux resulting from expansion from the historical Alpine focus can be deduced from the main profiles shared between these countries. All 33 EmsB profiles obtained from 528 samples from the nine eastern European countries belonged to the European clade, except one Asian profile form Ryazan Oblast, Russia. The expansion of E. multilocularis seems to have progressed from the historical Alpine focus through Hungary, Slovakia, the Czech Republic and southern Poland towards Latvia and Estonia. Most of the samples from Asia belong to the Asian clade, with one EmsB profile shared between Armenia and Turkey, and two between Turkey and Russia. However, two European profiles were described from two foxes in Turkey, including one harboring worms from both European and Asian clades. Three EmsB profiles from three Russian samples were associated with the Arctic clade. Two E. multilocularis profiles from rodents from Lake Baikal belonged to the Mongolian clade, described for the first time here using EmsB. Further worldwide studies on the genetic diversity of E. multilocularis using both mitochondrial sequencing and EmsB genotyping are needed to understand the distribution and expansion of the various clades. Article in Journal/Newspaper Arctic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic Infection, Genetics and Evolution 92 104863
institution Open Polar
collection Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe)
op_collection_id ftccsdartic
language English
topic Asia
Echinococcus multilocularis
EmsB microsatellite
Europe
Parasite expansion
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
spellingShingle Asia
Echinococcus multilocularis
EmsB microsatellite
Europe
Parasite expansion
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
Umhang, Gérald
Bastid, Vanessa
Avcioglu, Hamza
Bagrade, Guna
Bujanić, Miljenko
Bjelić Čabrilo, Oliveira
Casulli, Adriano
Dorny, Pierre
Van Der Giessen, Joke
Guven, Esin
Harna, Jiri
Karamon, Jacek
Kharchenko, Vitaliy
Knapp, Jenny
Kolarova, Libuse
Konyaev, Sergey
Laurimaa, Leidi
Losch, Serge
Miljević, Milan
Miterpakova, Martina
Moks, Epp
Romig, Thomas
Saarma, Urmas
Snabel, Viliam
Sreter, Tamas
Valdmann, Harri
Boué, Franck
Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker
topic_facet Asia
Echinococcus multilocularis
EmsB microsatellite
Europe
Parasite expansion
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
description International audience The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe helminthic zoonotic disease distributed in the Northern Hemisphere. The lifecycle of the parasite is mainly sylvatic, involving canid and rodent hosts. The absence of genetic data from most eastern European countries is a major knowledge gap, affecting the study of associations with parasite populations in Western Europe. In this study, EmsB microsatellite genotyping of E. multilocularis was performed to describe the genetic diversity and relatedness of 785 E. multilocularis isolates from four western and nine eastern European countries, as well as from Armenia and the Asian parts of Russia and Turkey. The presence of the same E. multilocularis populations in the Benelux resulting from expansion from the historical Alpine focus can be deduced from the main profiles shared between these countries. All 33 EmsB profiles obtained from 528 samples from the nine eastern European countries belonged to the European clade, except one Asian profile form Ryazan Oblast, Russia. The expansion of E. multilocularis seems to have progressed from the historical Alpine focus through Hungary, Slovakia, the Czech Republic and southern Poland towards Latvia and Estonia. Most of the samples from Asia belong to the Asian clade, with one EmsB profile shared between Armenia and Turkey, and two between Turkey and Russia. However, two European profiles were described from two foxes in Turkey, including one harboring worms from both European and Asian clades. Three EmsB profiles from three Russian samples were associated with the Arctic clade. Two E. multilocularis profiles from rodents from Lake Baikal belonged to the Mongolian clade, described for the first time here using EmsB. Further worldwide studies on the genetic diversity of E. multilocularis using both mitochondrial sequencing and EmsB genotyping are needed to understand the distribution and expansion of the various clades.
author2 Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE)
Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)
Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC)
format Article in Journal/Newspaper
author Umhang, Gérald
Bastid, Vanessa
Avcioglu, Hamza
Bagrade, Guna
Bujanić, Miljenko
Bjelić Čabrilo, Oliveira
Casulli, Adriano
Dorny, Pierre
Van Der Giessen, Joke
Guven, Esin
Harna, Jiri
Karamon, Jacek
Kharchenko, Vitaliy
Knapp, Jenny
Kolarova, Libuse
Konyaev, Sergey
Laurimaa, Leidi
Losch, Serge
Miljević, Milan
Miterpakova, Martina
Moks, Epp
Romig, Thomas
Saarma, Urmas
Snabel, Viliam
Sreter, Tamas
Valdmann, Harri
Boué, Franck
author_facet Umhang, Gérald
Bastid, Vanessa
Avcioglu, Hamza
Bagrade, Guna
Bujanić, Miljenko
Bjelić Čabrilo, Oliveira
Casulli, Adriano
Dorny, Pierre
Van Der Giessen, Joke
Guven, Esin
Harna, Jiri
Karamon, Jacek
Kharchenko, Vitaliy
Knapp, Jenny
Kolarova, Libuse
Konyaev, Sergey
Laurimaa, Leidi
Losch, Serge
Miljević, Milan
Miterpakova, Martina
Moks, Epp
Romig, Thomas
Saarma, Urmas
Snabel, Viliam
Sreter, Tamas
Valdmann, Harri
Boué, Franck
author_sort Umhang, Gérald
title Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker
title_short Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker
title_full Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker
title_fullStr Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker
title_full_unstemmed Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker
title_sort unravelling the genetic diversity and relatedness of echinococcus multilocularis isolates in eurasia using the emsb microsatellite nuclear marker
publisher HAL CCSD
publishDate 2021
url https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264
https://doi.org/10.1016/j.meegid.2021.104863
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source ISSN: 1567-1348
EISSN: 1567-7257
Infection, Genetics and Evolution
https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264
Infection, Genetics and Evolution, Elsevier, 2021, 92, pp.104863. ⟨10.1016/j.meegid.2021.104863⟩
op_relation info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2021.104863
info:eu-repo/semantics/altIdentifier/pmid/33857665
hal-03516264
https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264
doi:10.1016/j.meegid.2021.104863
PUBMED: 33857665
op_doi https://doi.org/10.1016/j.meegid.2021.104863
container_title Infection, Genetics and Evolution
container_volume 92
container_start_page 104863
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