Response of bacterioplankton to iron fertilization in the Southern Ocean
We studied the bacterial response to Fe fertilization over 3 weeks during the second iron-enrichment experiment (EisenEx) in the Southern Ocean. Bacterial abundance in the Fe-fertilized patch increased over the first 12 d following Fe release and remained about twice as high as outside the Fe-fertil...
Published in: | Limnology and Oceanography |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hal.archives-ouvertes.fr/hal-03506677 https://doi.org/10.4319/lo.2004.49.3.0799 |
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ftccsdartic:oai:HAL:hal-03506677v1 2023-05-15T18:25:09+02:00 Response of bacterioplankton to iron fertilization in the Southern Ocean Arrieta, JM Weinbauer, MG Lute, C Herndl, GJ Laboratoire d'océanographie de Villefranche (LOV) Observatoire océanologique de Villefranche-sur-mer (OOVM) Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) 2004 https://hal.archives-ouvertes.fr/hal-03506677 https://doi.org/10.4319/lo.2004.49.3.0799 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.4319/lo.2004.49.3.0799 hal-03506677 https://hal.archives-ouvertes.fr/hal-03506677 doi:10.4319/lo.2004.49.3.0799 LIMNOLOGY AND OCEANOGRAPHY https://hal.archives-ouvertes.fr/hal-03506677 LIMNOLOGY AND OCEANOGRAPHY, 2004, 49 (3), pp.799-808. ⟨10.4319/lo.2004.49.3.0799⟩ [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2004 ftccsdartic https://doi.org/10.4319/lo.2004.49.3.0799 2022-01-08T23:26:35Z We studied the bacterial response to Fe fertilization over 3 weeks during the second iron-enrichment experiment (EisenEx) in the Southern Ocean. Bacterial abundance in the Fe-fertilized patch increased over the first 12 d following Fe release and remained about twice as high as outside the Fe-fertilized patch until the end of the experiment. Bacterial production peaked a few days after each of the three Fe releases inside the Fe-fertilized patch, reaching rates two to three times higher than outside the patch. Besides the peaks in leucine and thymidine incorporation following Fe release, bacterial production was not significantly higher inside the patch than outside, suggesting direct limitation of bacterial growth by Fe. Bacterial aminopeptidase activity roughly followed the increase in bacterial abundance, whereas cell-specific alpha- and beta-glucosidase were higher inside the Fe-fertilized patch. The diversity of beta-glucosidases was determined by capillary electrophoresis zymography. The different beta-glucosidases showed much higher activity levels inside the patch than in the surrounding waters, and three additional beta-glucosidases constituting similar to55% of the total beta-glucosidase activity were present inside the Fe-fertilized patch from day 9 onward. No major changes in response to Fe fertilization were detected in the phylogenetic composition of the bacterioplankton community, as determined by 16S rDNA fingerprinting, indicating a remarkable adaptation of the bacterioplankton community to episodic iron inputs. This stability on the phylogenetic level is contrasted by the dramatic qualitative and quantitative changes in ectoenzymatic activity. Article in Journal/Newspaper Southern Ocean Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Southern Ocean Limnology and Oceanography 49 3 799 808 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Arrieta, JM Weinbauer, MG Lute, C Herndl, GJ Response of bacterioplankton to iron fertilization in the Southern Ocean |
topic_facet |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
We studied the bacterial response to Fe fertilization over 3 weeks during the second iron-enrichment experiment (EisenEx) in the Southern Ocean. Bacterial abundance in the Fe-fertilized patch increased over the first 12 d following Fe release and remained about twice as high as outside the Fe-fertilized patch until the end of the experiment. Bacterial production peaked a few days after each of the three Fe releases inside the Fe-fertilized patch, reaching rates two to three times higher than outside the patch. Besides the peaks in leucine and thymidine incorporation following Fe release, bacterial production was not significantly higher inside the patch than outside, suggesting direct limitation of bacterial growth by Fe. Bacterial aminopeptidase activity roughly followed the increase in bacterial abundance, whereas cell-specific alpha- and beta-glucosidase were higher inside the Fe-fertilized patch. The diversity of beta-glucosidases was determined by capillary electrophoresis zymography. The different beta-glucosidases showed much higher activity levels inside the patch than in the surrounding waters, and three additional beta-glucosidases constituting similar to55% of the total beta-glucosidase activity were present inside the Fe-fertilized patch from day 9 onward. No major changes in response to Fe fertilization were detected in the phylogenetic composition of the bacterioplankton community, as determined by 16S rDNA fingerprinting, indicating a remarkable adaptation of the bacterioplankton community to episodic iron inputs. This stability on the phylogenetic level is contrasted by the dramatic qualitative and quantitative changes in ectoenzymatic activity. |
author2 |
Laboratoire d'océanographie de Villefranche (LOV) Observatoire océanologique de Villefranche-sur-mer (OOVM) Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Arrieta, JM Weinbauer, MG Lute, C Herndl, GJ |
author_facet |
Arrieta, JM Weinbauer, MG Lute, C Herndl, GJ |
author_sort |
Arrieta, JM |
title |
Response of bacterioplankton to iron fertilization in the Southern Ocean |
title_short |
Response of bacterioplankton to iron fertilization in the Southern Ocean |
title_full |
Response of bacterioplankton to iron fertilization in the Southern Ocean |
title_fullStr |
Response of bacterioplankton to iron fertilization in the Southern Ocean |
title_full_unstemmed |
Response of bacterioplankton to iron fertilization in the Southern Ocean |
title_sort |
response of bacterioplankton to iron fertilization in the southern ocean |
publisher |
HAL CCSD |
publishDate |
2004 |
url |
https://hal.archives-ouvertes.fr/hal-03506677 https://doi.org/10.4319/lo.2004.49.3.0799 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
LIMNOLOGY AND OCEANOGRAPHY https://hal.archives-ouvertes.fr/hal-03506677 LIMNOLOGY AND OCEANOGRAPHY, 2004, 49 (3), pp.799-808. ⟨10.4319/lo.2004.49.3.0799⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.4319/lo.2004.49.3.0799 hal-03506677 https://hal.archives-ouvertes.fr/hal-03506677 doi:10.4319/lo.2004.49.3.0799 |
op_doi |
https://doi.org/10.4319/lo.2004.49.3.0799 |
container_title |
Limnology and Oceanography |
container_volume |
49 |
container_issue |
3 |
container_start_page |
799 |
op_container_end_page |
808 |
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1766206392518049792 |