Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red
This study defines the conditions (wavelength selection and sensitivity) for spectrofluorometric quantification of neutral and polar lipids using Nile red (NR). The fluorescence intensity of the probe with standard lipids was examined in relation to lipid concentration and composition. Standards use...
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Online Access: | https://hal.archives-ouvertes.fr/hal-03504550 https://doi.org/10.1016/S0304-4203(99)00075-4 |
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ftccsdartic:oai:HAL:hal-03504550v1 2023-05-15T13:32:49+02:00 Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red Alonzo, F MAYZAUD, P Station Zoologique de Villefranche Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) 1999 https://hal.archives-ouvertes.fr/hal-03504550 https://doi.org/10.1016/S0304-4203(99)00075-4 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1016/S0304-4203(99)00075-4 hal-03504550 https://hal.archives-ouvertes.fr/hal-03504550 doi:10.1016/S0304-4203(99)00075-4 MARINE CHEMISTRY https://hal.archives-ouvertes.fr/hal-03504550 MARINE CHEMISTRY, 1999, 67 (3-4), pp.289-301. ⟨10.1016/S0304-4203(99)00075-4⟩ [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 1999 ftccsdartic https://doi.org/10.1016/S0304-4203(99)00075-4 2022-01-01T23:22:37Z This study defines the conditions (wavelength selection and sensitivity) for spectrofluorometric quantification of neutral and polar lipids using Nile red (NR). The fluorescence intensity of the probe with standard lipids was examined in relation to lipid concentration and composition. Standards used included commercial triolein (TO) and phosphatidylcholine (PC), triacylglycerols (TG) and phospholipids (PL) from the krill Euphausia sllperba and Meganyctiphanes norvegica, and wax esters (WE) and PL from the copepods Paraeuchaeta antarctica and Drepanopus pectinatus. At any wavelength tested, polar and neutral lipids could be detected and fluorescence overlapped resulting in an interference of 10% to 20%. This interference between polar and neutral lipids was quantified for the standardisation. Wavelength selection for maximum emission with neutral and polar lipid classes achieved maximal sensitivity. Relationships between fluorescence intensity and lipid concentration were shown to be linear and specific to the Lipid used. Large errors occurred when calibrations were performed with commercial lipids or with lipids obtained from other plankton species. Therefore, only the use of specific lipids as standards provided simultaneous and absolute quantification of neutral and polar lipids (relative errors were lower than 5%). The presence of PL in the sample (a ratio as low as 1:20) improved fluorescence of NR with TG by enhancing the solubility of the lipid microemulsion in water. (C) 1999 Elsevier Science B.V. All rights reserved. Article in Journal/Newspaper Antarc* Antarctica Meganyctiphanes norvegica Copepods Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Marine Chemistry 67 3-4 289 301 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Alonzo, F MAYZAUD, P Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red |
topic_facet |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
This study defines the conditions (wavelength selection and sensitivity) for spectrofluorometric quantification of neutral and polar lipids using Nile red (NR). The fluorescence intensity of the probe with standard lipids was examined in relation to lipid concentration and composition. Standards used included commercial triolein (TO) and phosphatidylcholine (PC), triacylglycerols (TG) and phospholipids (PL) from the krill Euphausia sllperba and Meganyctiphanes norvegica, and wax esters (WE) and PL from the copepods Paraeuchaeta antarctica and Drepanopus pectinatus. At any wavelength tested, polar and neutral lipids could be detected and fluorescence overlapped resulting in an interference of 10% to 20%. This interference between polar and neutral lipids was quantified for the standardisation. Wavelength selection for maximum emission with neutral and polar lipid classes achieved maximal sensitivity. Relationships between fluorescence intensity and lipid concentration were shown to be linear and specific to the Lipid used. Large errors occurred when calibrations were performed with commercial lipids or with lipids obtained from other plankton species. Therefore, only the use of specific lipids as standards provided simultaneous and absolute quantification of neutral and polar lipids (relative errors were lower than 5%). The presence of PL in the sample (a ratio as low as 1:20) improved fluorescence of NR with TG by enhancing the solubility of the lipid microemulsion in water. (C) 1999 Elsevier Science B.V. All rights reserved. |
author2 |
Station Zoologique de Villefranche Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Alonzo, F MAYZAUD, P |
author_facet |
Alonzo, F MAYZAUD, P |
author_sort |
Alonzo, F |
title |
Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red |
title_short |
Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red |
title_full |
Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red |
title_fullStr |
Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red |
title_full_unstemmed |
Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red |
title_sort |
spectrofluorometric quantification of neutral and polar lipids in zooplankton using nile red |
publisher |
HAL CCSD |
publishDate |
1999 |
url |
https://hal.archives-ouvertes.fr/hal-03504550 https://doi.org/10.1016/S0304-4203(99)00075-4 |
genre |
Antarc* Antarctica Meganyctiphanes norvegica Copepods |
genre_facet |
Antarc* Antarctica Meganyctiphanes norvegica Copepods |
op_source |
MARINE CHEMISTRY https://hal.archives-ouvertes.fr/hal-03504550 MARINE CHEMISTRY, 1999, 67 (3-4), pp.289-301. ⟨10.1016/S0304-4203(99)00075-4⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0304-4203(99)00075-4 hal-03504550 https://hal.archives-ouvertes.fr/hal-03504550 doi:10.1016/S0304-4203(99)00075-4 |
op_doi |
https://doi.org/10.1016/S0304-4203(99)00075-4 |
container_title |
Marine Chemistry |
container_volume |
67 |
container_issue |
3-4 |
container_start_page |
289 |
op_container_end_page |
301 |
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1766036359119634432 |