Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism

International audience The biochemical pathways of anaerobic sulfur disproportionation are only partially deciphered, and the mechanisms involved in the first step of S0-disproportionation remain unknown. Here, we present the results of sequencing and analysis of the complete genome of Dissulfurimic...

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Published in:Microorganisms
Main Authors: Yvenou, Stéven, Allioux, Maxime, Slobodkin, Alexander, Slobodkina, Galina, Jebbar, Mohamed, Alain, Karine
Other Authors: Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2021
Subjects:
G
Online Access:https://hal.science/hal-03504518
https://hal.science/hal-03504518/document
https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf
https://doi.org/10.3390/microorganisms10010060
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spelling ftccsdartic:oai:HAL:hal-03504518v1 2024-02-27T08:42:27+00:00 Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism Yvenou, Stéven Allioux, Maxime Slobodkin, Alexander Slobodkina, Galina Jebbar, Mohamed Alain, Karine Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017) 2021-12-28 https://hal.science/hal-03504518 https://hal.science/hal-03504518/document https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf https://doi.org/10.3390/microorganisms10010060 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10010060 hal-03504518 https://hal.science/hal-03504518 https://hal.science/hal-03504518/document https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf doi:10.3390/microorganisms10010060 info:eu-repo/semantics/OpenAccess ISSN: 2076-2607 Microorganisms https://hal.science/hal-03504518 Microorganisms, 2021, 10, ⟨10.3390/microorganisms10010060⟩ G Sulfur-Disproportionating hydrothermal vent genomics thermophile [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2021 ftccsdartic https://doi.org/10.3390/microorganisms10010060 2024-01-28T01:34:37Z International audience The biochemical pathways of anaerobic sulfur disproportionation are only partially deciphered, and the mechanisms involved in the first step of S0-disproportionation remain unknown. Here, we present the results of sequencing and analysis of the complete genome of Dissulfurimicrobium hydrothermale strain Sh68T, one of two strains isolated to date known to grow exclusively by anaerobic disproportionation of inorganic sulfur compounds. Dissulfurimicrobium hydrothermale Sh68T is a motile, thermophilic, anaerobic, chemolithoautotrophic microorganism isolated from a hydrothermal pond at Uzon caldera, Kamchatka, Russia. It is able to produce energy and grow by disproportionation of elemental sulfur, sulfite and thiosulfate. Its genome consists of a circular chromosome of 2,025,450 base pairs, has a G + C content of 49.66% and a completion of 97.6%. Genomic data suggest that CO2 assimilation is carried out by the Wood–Ljungdhal pathway and that central anabolism involves the gluconeogenesis pathway. The genome of strain Sh68T encodes the complete gene set of the dissimilatory sulfate reduction pathway, some of which are likely to be involved in sulfur disproportionation. A short sequence protein of unknown function present in the genome of strain Sh68T is conserved in the genomes of a large panel of other S0-disproportionating bacteria and was absent from the genomes of microorganisms incapable of elemental sulfur disproportionation. We propose that this protein may be involved in the first step of elemental sulfur disproportionation, as S0 is poorly soluble and unable to cross the cytoplasmic membrane in this form Article in Journal/Newspaper Kamchatka Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Microorganisms 10 1 60
institution Open Polar
collection Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe)
op_collection_id ftccsdartic
language English
topic G
Sulfur-Disproportionating
hydrothermal vent
genomics
thermophile
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
spellingShingle G
Sulfur-Disproportionating
hydrothermal vent
genomics
thermophile
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Yvenou, Stéven
Allioux, Maxime
Slobodkin, Alexander
Slobodkina, Galina
Jebbar, Mohamed
Alain, Karine
Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism
topic_facet G
Sulfur-Disproportionating
hydrothermal vent
genomics
thermophile
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
description International audience The biochemical pathways of anaerobic sulfur disproportionation are only partially deciphered, and the mechanisms involved in the first step of S0-disproportionation remain unknown. Here, we present the results of sequencing and analysis of the complete genome of Dissulfurimicrobium hydrothermale strain Sh68T, one of two strains isolated to date known to grow exclusively by anaerobic disproportionation of inorganic sulfur compounds. Dissulfurimicrobium hydrothermale Sh68T is a motile, thermophilic, anaerobic, chemolithoautotrophic microorganism isolated from a hydrothermal pond at Uzon caldera, Kamchatka, Russia. It is able to produce energy and grow by disproportionation of elemental sulfur, sulfite and thiosulfate. Its genome consists of a circular chromosome of 2,025,450 base pairs, has a G + C content of 49.66% and a completion of 97.6%. Genomic data suggest that CO2 assimilation is carried out by the Wood–Ljungdhal pathway and that central anabolism involves the gluconeogenesis pathway. The genome of strain Sh68T encodes the complete gene set of the dissimilatory sulfate reduction pathway, some of which are likely to be involved in sulfur disproportionation. A short sequence protein of unknown function present in the genome of strain Sh68T is conserved in the genomes of a large panel of other S0-disproportionating bacteria and was absent from the genomes of microorganisms incapable of elemental sulfur disproportionation. We propose that this protein may be involved in the first step of elemental sulfur disproportionation, as S0 is poorly soluble and unable to cross the cytoplasmic membrane in this form
author2 Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E)
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)
ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017)
format Article in Journal/Newspaper
author Yvenou, Stéven
Allioux, Maxime
Slobodkin, Alexander
Slobodkina, Galina
Jebbar, Mohamed
Alain, Karine
author_facet Yvenou, Stéven
Allioux, Maxime
Slobodkin, Alexander
Slobodkina, Galina
Jebbar, Mohamed
Alain, Karine
author_sort Yvenou, Stéven
title Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism
title_short Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism
title_full Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism
title_fullStr Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism
title_full_unstemmed Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism
title_sort genetic potential of dissulfurimicrobium hydrothermale, an obligate sulfur-disproportionating thermophilic microorganism
publisher HAL CCSD
publishDate 2021
url https://hal.science/hal-03504518
https://hal.science/hal-03504518/document
https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf
https://doi.org/10.3390/microorganisms10010060
genre Kamchatka
genre_facet Kamchatka
op_source ISSN: 2076-2607
Microorganisms
https://hal.science/hal-03504518
Microorganisms, 2021, 10, ⟨10.3390/microorganisms10010060⟩
op_relation info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10010060
hal-03504518
https://hal.science/hal-03504518
https://hal.science/hal-03504518/document
https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf
doi:10.3390/microorganisms10010060
op_rights info:eu-repo/semantics/OpenAccess
op_doi https://doi.org/10.3390/microorganisms10010060
container_title Microorganisms
container_volume 10
container_issue 1
container_start_page 60
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