Response of bacterioplankton community structure to an artificial gradient of pCO(2) in the Arctic Ocean

• Published in: Biogeosciences
• Main Authors: Zhang, R., Xia, X., Lau, S. C. K., Motegi, C., Weinbauer, M. G., Jiao, N.
• Other Authors: Chinese Academy of Meteorological Sciences (CAMS), Centre Interdisciplinaire de Nanoscience de Marseille (CINaM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'océanographie de Villefranche (LOV), Observatoire océanologique de Villefranche-sur-mer (OOVM), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2013
• Subjects: [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography; Arctic; Arctic Ocean; Kings Bay; Kongsfjord*; Kongsfjorden; Ocean acidification; Spitsbergen
• Online Access: https://hal.archives-ouvertes.fr/hal-03502643; https://hal.archives-ouvertes.fr/hal-03502643/document; https://hal.archives-ouvertes.fr/hal-03502643/file/bg-10-3679-2013.pdf; https://doi.org/10.5194/bg-10-3679-2013

International audience In order to test the influences of ocean acidification on the ocean pelagic ecosystem, so far the largest CO2 manipulation mesocosm study (European Project on Ocean Acidification, EPOCA) was performed in Kings Bay (Kongsfjorden), Spitsbergen. During a 30 day incubation, bacterial diversity was investigated using DNA fingerprinting and clone library analysis of bacterioplankton samples. Terminal restriction fragment length polymorphism (T-RFLP) analysis of the PCR amplicons of the 16S rRNA genes revealed that general bacterial diversity, taxonomic richness and community structure were influenced by the variation of productivity during the time of incubation, but not the degree of ocean acidification. A BIOENV analysis suggested a complex control of bacterial community structure by various biological and chemical environmental parameters. The maximum apparent diversity of bacterioplankton (i.e., the number of T-RFs) in high and low pCO(2) treatments differed significantly. A negative relationship between the relative abundance of Bacteroidetes and pCO(2) levels was observed for samples at the end of the experiment by the combination of T-RFLP and clone library analysis. Our study suggests that ocean acidification affects the development of bacterial assemblages and potentially impacts the ecological function of the bacterioplankton in the marine ecosystem.