High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue
International audience The zebrafish is extensively used as an animal model for human and fish diseases. However, our understanding of the structural organization of its immune system remains incomplete, especially the mucosa-associated lymphoid tissues (MALTs). Teleost MALTs are commonly perceived...
Published in: | Frontiers in Immunology |
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Main Authors: | , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2021
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Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-03487856 https://hal.archives-ouvertes.fr/hal-03487856/document https://hal.archives-ouvertes.fr/hal-03487856/file/2021_Dalum_fimmu.pdf https://doi.org/10.3389/fimmu.2021.769901 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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English |
topic |
ALT GIALT gills ILT immunology lymphoid tissue mucosal immunity zebrafish [SDV.IMM]Life Sciences [q-bio]/Immunology |
spellingShingle |
ALT GIALT gills ILT immunology lymphoid tissue mucosal immunity zebrafish [SDV.IMM]Life Sciences [q-bio]/Immunology Dalum, Alf Seljenes Kraus, Aurora L. Khan, Shanawaz Davydova, Erna Rigaudeau, Dimitri Bjørgen, Håvard López-Porras, Adrián Griffiths, Gareth Wyn Wiegertjes, Geert Frits Koppang, Erling Olaf Laf Salinas, Irene Boudinot, Pierre Rességuier, Julien High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
topic_facet |
ALT GIALT gills ILT immunology lymphoid tissue mucosal immunity zebrafish [SDV.IMM]Life Sciences [q-bio]/Immunology |
description |
International audience The zebrafish is extensively used as an animal model for human and fish diseases. However, our understanding of the structural organization of its immune system remains incomplete, especially the mucosa-associated lymphoid tissues (MALTs). Teleost MALTs are commonly perceived as diffuse and scattered populations of immune cells throughout the mucosa. Yet, structured MALTs have been recently discovered in Atlantic salmon (Salmo salar L.), including the interbranchial lymphoid tissue (ILT) in the gills. The existence of the ILT was only recently identified in zebrafish and other fish species, highlighting the need for in-depth characterizations of the gill-associated lymphoid tissue (GIALT) in teleosts. Here, using 3-D high-resolution microscopy, we analyze the GIALT of adult zebrafish with an immuno-histology approach that reveals the organization of lymphoid tissues via the labeling of T/NK cells with an antibody directed to a highly conserved epitope on the kinase ZAP70. We show that the GIALT in zebrafish is distributed over at least five distinct sub-regions, an organization found in all pairs of gill arches. The GIALT is diffuse in the pharyngeal part of the gill arch, the interbranchial septum and the filaments/lamellae, and structured in two sub-regions: the ILT, and a newly discovered lymphoid structure located along each side of the gill arch, which we named the Amphibranchial Lymphoid Tissue (ALT). Based on RAG2 expression, neither the ILT nor the ALT constitute additional thymi. The ALT shares several features with the ILT such as presence of abundant lymphoid cells and myeloid cells embedded in a network of reticulated epithelial cells. Further, the ILT and the ALT are also a site for T/NK cell proliferation. Both ILT and ALT show structural changes after infection with Spring Viraemia of Carp Virus (SVCV). Together, these data suggest that ALT and ILT play an active role in immune responses. Comparative studies show that whereas the ILT seems absent in most neoteleosts ... |
author2 |
Norwegian Institute of Food,Fisheries and Aquaculture Research (NOFIMA) Center for Evolutionary and Theoretical Immunology (CETI) The University of New Mexico Albuquerque University of Oslo (UiO) Institut des Etudes Régionales et du patrimoine (IERP (EA 3723)) Université Jean Monnet Saint-Étienne (UJM) Norwegian University of Life Sciences (NMBU) Wageningen University and Research Wageningen (WUR) Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Norges Forskningsråd: FRIMEDBIO-No 144642, INFRA-2016-1 731014 We thank the Norwegian Research Council for funding (FRIMEDBIO-No 144642). In addition, PB was supported by the VetBioNet project (EU Grant Agreement INFRA-2016-1 731014) and by institutional grants from INRAE. |
format |
Article in Journal/Newspaper |
author |
Dalum, Alf Seljenes Kraus, Aurora L. Khan, Shanawaz Davydova, Erna Rigaudeau, Dimitri Bjørgen, Håvard López-Porras, Adrián Griffiths, Gareth Wyn Wiegertjes, Geert Frits Koppang, Erling Olaf Laf Salinas, Irene Boudinot, Pierre Rességuier, Julien |
author_facet |
Dalum, Alf Seljenes Kraus, Aurora L. Khan, Shanawaz Davydova, Erna Rigaudeau, Dimitri Bjørgen, Håvard López-Porras, Adrián Griffiths, Gareth Wyn Wiegertjes, Geert Frits Koppang, Erling Olaf Laf Salinas, Irene Boudinot, Pierre Rességuier, Julien |
author_sort |
Dalum, Alf Seljenes |
title |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_short |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_full |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_fullStr |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_full_unstemmed |
High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue |
title_sort |
high-resolution, 3d imaging of the zebrafish gill-associated lymphoid tissue (gialt) reveals a novel lymphoid structure, the amphibranchial lymphoid tissue |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.archives-ouvertes.fr/hal-03487856 https://hal.archives-ouvertes.fr/hal-03487856/document https://hal.archives-ouvertes.fr/hal-03487856/file/2021_Dalum_fimmu.pdf https://doi.org/10.3389/fimmu.2021.769901 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 1664-3224 EISSN: 1664-3224 Frontiers in Immunology https://hal.archives-ouvertes.fr/hal-03487856 Frontiers in Immunology, Frontiers, 2021, 12, ⟨10.3389/fimmu.2021.769901⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2021.769901 info:eu-repo/semantics/altIdentifier/pmid/34880866 hal-03487856 https://hal.archives-ouvertes.fr/hal-03487856 https://hal.archives-ouvertes.fr/hal-03487856/document https://hal.archives-ouvertes.fr/hal-03487856/file/2021_Dalum_fimmu.pdf doi:10.3389/fimmu.2021.769901 PUBMED: 34880866 WOS: 000727190800001 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3389/fimmu.2021.769901 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
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1766363488713703424 |
spelling |
ftccsdartic:oai:HAL:hal-03487856v1 2023-05-15T15:33:01+02:00 High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue Dalum, Alf Seljenes Kraus, Aurora L. Khan, Shanawaz Davydova, Erna Rigaudeau, Dimitri Bjørgen, Håvard López-Porras, Adrián Griffiths, Gareth Wyn Wiegertjes, Geert Frits Koppang, Erling Olaf Laf Salinas, Irene Boudinot, Pierre Rességuier, Julien Norwegian Institute of Food,Fisheries and Aquaculture Research (NOFIMA) Center for Evolutionary and Theoretical Immunology (CETI) The University of New Mexico Albuquerque University of Oslo (UiO) Institut des Etudes Régionales et du patrimoine (IERP (EA 3723)) Université Jean Monnet Saint-Étienne (UJM) Norwegian University of Life Sciences (NMBU) Wageningen University and Research Wageningen (WUR) Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Norges Forskningsråd: FRIMEDBIO-No 144642, INFRA-2016-1 731014 We thank the Norwegian Research Council for funding (FRIMEDBIO-No 144642). In addition, PB was supported by the VetBioNet project (EU Grant Agreement INFRA-2016-1 731014) and by institutional grants from INRAE. 2021 https://hal.archives-ouvertes.fr/hal-03487856 https://hal.archives-ouvertes.fr/hal-03487856/document https://hal.archives-ouvertes.fr/hal-03487856/file/2021_Dalum_fimmu.pdf https://doi.org/10.3389/fimmu.2021.769901 en eng HAL CCSD Frontiers info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2021.769901 info:eu-repo/semantics/altIdentifier/pmid/34880866 hal-03487856 https://hal.archives-ouvertes.fr/hal-03487856 https://hal.archives-ouvertes.fr/hal-03487856/document https://hal.archives-ouvertes.fr/hal-03487856/file/2021_Dalum_fimmu.pdf doi:10.3389/fimmu.2021.769901 PUBMED: 34880866 WOS: 000727190800001 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1664-3224 EISSN: 1664-3224 Frontiers in Immunology https://hal.archives-ouvertes.fr/hal-03487856 Frontiers in Immunology, Frontiers, 2021, 12, ⟨10.3389/fimmu.2021.769901⟩ ALT GIALT gills ILT immunology lymphoid tissue mucosal immunity zebrafish [SDV.IMM]Life Sciences [q-bio]/Immunology info:eu-repo/semantics/article Journal articles 2021 ftccsdartic https://doi.org/10.3389/fimmu.2021.769901 2022-01-08T23:31:19Z International audience The zebrafish is extensively used as an animal model for human and fish diseases. However, our understanding of the structural organization of its immune system remains incomplete, especially the mucosa-associated lymphoid tissues (MALTs). Teleost MALTs are commonly perceived as diffuse and scattered populations of immune cells throughout the mucosa. Yet, structured MALTs have been recently discovered in Atlantic salmon (Salmo salar L.), including the interbranchial lymphoid tissue (ILT) in the gills. The existence of the ILT was only recently identified in zebrafish and other fish species, highlighting the need for in-depth characterizations of the gill-associated lymphoid tissue (GIALT) in teleosts. Here, using 3-D high-resolution microscopy, we analyze the GIALT of adult zebrafish with an immuno-histology approach that reveals the organization of lymphoid tissues via the labeling of T/NK cells with an antibody directed to a highly conserved epitope on the kinase ZAP70. We show that the GIALT in zebrafish is distributed over at least five distinct sub-regions, an organization found in all pairs of gill arches. The GIALT is diffuse in the pharyngeal part of the gill arch, the interbranchial septum and the filaments/lamellae, and structured in two sub-regions: the ILT, and a newly discovered lymphoid structure located along each side of the gill arch, which we named the Amphibranchial Lymphoid Tissue (ALT). Based on RAG2 expression, neither the ILT nor the ALT constitute additional thymi. The ALT shares several features with the ILT such as presence of abundant lymphoid cells and myeloid cells embedded in a network of reticulated epithelial cells. Further, the ILT and the ALT are also a site for T/NK cell proliferation. Both ILT and ALT show structural changes after infection with Spring Viraemia of Carp Virus (SVCV). Together, these data suggest that ALT and ILT play an active role in immune responses. Comparative studies show that whereas the ILT seems absent in most neoteleosts ... Article in Journal/Newspaper Atlantic salmon Salmo salar Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Frontiers in Immunology 12 |