Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation
International audience In the dark ocean, the balance between the heterotrophic carbon demand and the supply of sinking carbon through the biological carbon pump remains poorly constrained. In situ tracking of the dynamics of microbial degradation processes occurring on the gravitational sinking par...
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Online Access: | https://hal.archives-ouvertes.fr/hal-03435789 https://doi.org/10.3390/w13192616 |
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ftccsdartic:oai:HAL:hal-03435789v1 2023-05-15T17:35:30+02:00 Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation Tamburini, Christian Garel, Marc Barani, Aude Boeuf, Dominique Bonin, Patricia Bhairy, Nagib Guasco, Sophie Jacquet, Stéphanie Le Moigne, Frédéric Panagiotopoulos, Christos Riou, Virginie Veloso, Sandrine Santinelli, Chiara Armougom, Fabrice MEDITERRANEAN INSTITUTE OF OCEANOGRAPHY MARSEILLE FRA Partenaires IRSTEA Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) 2021-10 https://hal.archives-ouvertes.fr/hal-03435789 https://doi.org/10.3390/w13192616 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/w13192616 hal-03435789 https://hal.archives-ouvertes.fr/hal-03435789 doi:10.3390/w13192616 ISSN: 2073-4441 Water https://hal.archives-ouvertes.fr/hal-03435789 Water, MDPI, 2021, 13 (19), pp.2616. ⟨10.3390/w13192616⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2021 ftccsdartic https://doi.org/10.3390/w13192616 2021-11-20T23:22:58Z International audience In the dark ocean, the balance between the heterotrophic carbon demand and the supply of sinking carbon through the biological carbon pump remains poorly constrained. In situ tracking of the dynamics of microbial degradation processes occurring on the gravitational sinking particles is still challenging. Our particle sinking simulator system (PASS) intends to mimic as closely as possible the in situ variations in pressure and temperature experienced by gravitational sinking particles. Here, we used the PASS to simultaneously track geochemical and microbial changes that occurred during the sinking through the mesopelagic zone of laboratory-grown Emiliania huxleyi aggregates amended by a natural microbial community sampled at 105 m depth in the North Atlantic Ocean. The impact of pressure on the prokaryotic degradation of POC and dissolution of E. huxleyi-derived calcite was not marked compared to atmospheric pressure. In contrast, using global O2 consumption monitored in real-time inside the high-pressure bottles using planar optodes via a sapphire window, a reduction of respiration rate was recorded in surface-originated community assemblages under increasing pressure conditions. Moreover, using a 16S rRNA metabarcoding survey, we demonstrated a drastic difference in transcriptionally active prokaryotes associated with particles, incubated either at atmospheric pressure or under linearly increasing hydrostatic pressure conditions. The increase in hydrostatic pressure reduced both the phylogenetic diversity and the species richness. The incubation at atmospheric pressure, however, promoted an opportunistic community of “fast” degraders from the surface (Saccharospirillaceae, Hyphomonadaceae, and Pseudoalteromonadaceae), known to be associated with surface phytoplankton blooms. In contrast, the incubation under increasing pressure condition incubations revealed an increase in the particle colonizer families Flavobacteriaceae and Rhodobacteraceae, and also Colwelliaceae, which are known to ... Article in Journal/Newspaper North Atlantic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Water 13 19 2616 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Tamburini, Christian Garel, Marc Barani, Aude Boeuf, Dominique Bonin, Patricia Bhairy, Nagib Guasco, Sophie Jacquet, Stéphanie Le Moigne, Frédéric Panagiotopoulos, Christos Riou, Virginie Veloso, Sandrine Santinelli, Chiara Armougom, Fabrice Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience In the dark ocean, the balance between the heterotrophic carbon demand and the supply of sinking carbon through the biological carbon pump remains poorly constrained. In situ tracking of the dynamics of microbial degradation processes occurring on the gravitational sinking particles is still challenging. Our particle sinking simulator system (PASS) intends to mimic as closely as possible the in situ variations in pressure and temperature experienced by gravitational sinking particles. Here, we used the PASS to simultaneously track geochemical and microbial changes that occurred during the sinking through the mesopelagic zone of laboratory-grown Emiliania huxleyi aggregates amended by a natural microbial community sampled at 105 m depth in the North Atlantic Ocean. The impact of pressure on the prokaryotic degradation of POC and dissolution of E. huxleyi-derived calcite was not marked compared to atmospheric pressure. In contrast, using global O2 consumption monitored in real-time inside the high-pressure bottles using planar optodes via a sapphire window, a reduction of respiration rate was recorded in surface-originated community assemblages under increasing pressure conditions. Moreover, using a 16S rRNA metabarcoding survey, we demonstrated a drastic difference in transcriptionally active prokaryotes associated with particles, incubated either at atmospheric pressure or under linearly increasing hydrostatic pressure conditions. The increase in hydrostatic pressure reduced both the phylogenetic diversity and the species richness. The incubation at atmospheric pressure, however, promoted an opportunistic community of “fast” degraders from the surface (Saccharospirillaceae, Hyphomonadaceae, and Pseudoalteromonadaceae), known to be associated with surface phytoplankton blooms. In contrast, the incubation under increasing pressure condition incubations revealed an increase in the particle colonizer families Flavobacteriaceae and Rhodobacteraceae, and also Colwelliaceae, which are known to ... |
author2 |
MEDITERRANEAN INSTITUTE OF OCEANOGRAPHY MARSEILLE FRA Partenaires IRSTEA Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) |
format |
Article in Journal/Newspaper |
author |
Tamburini, Christian Garel, Marc Barani, Aude Boeuf, Dominique Bonin, Patricia Bhairy, Nagib Guasco, Sophie Jacquet, Stéphanie Le Moigne, Frédéric Panagiotopoulos, Christos Riou, Virginie Veloso, Sandrine Santinelli, Chiara Armougom, Fabrice |
author_facet |
Tamburini, Christian Garel, Marc Barani, Aude Boeuf, Dominique Bonin, Patricia Bhairy, Nagib Guasco, Sophie Jacquet, Stéphanie Le Moigne, Frédéric Panagiotopoulos, Christos Riou, Virginie Veloso, Sandrine Santinelli, Chiara Armougom, Fabrice |
author_sort |
Tamburini, Christian |
title |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_short |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_full |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_fullStr |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_full_unstemmed |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_sort |
increasing hydrostatic pressure impacts the prokaryotic diversity during emiliania huxleyi aggregates degradation |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.archives-ouvertes.fr/hal-03435789 https://doi.org/10.3390/w13192616 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 2073-4441 Water https://hal.archives-ouvertes.fr/hal-03435789 Water, MDPI, 2021, 13 (19), pp.2616. ⟨10.3390/w13192616⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/w13192616 hal-03435789 https://hal.archives-ouvertes.fr/hal-03435789 doi:10.3390/w13192616 |
op_doi |
https://doi.org/10.3390/w13192616 |
container_title |
Water |
container_volume |
13 |
container_issue |
19 |
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2616 |
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1766134692680040448 |