Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate
International audience Viral entry mechanisms of herpesviruses constitute a highly complex process which implicates several viral glycoproteins and different receptors on the host cell surfaces. This initial infection stage was currently undescribed for Ostreid herpes virus 1 (OsHV-1), a herpesvirus...
Published in: | Frontiers in Microbiology |
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Online Access: | https://hal-normandie-univ.archives-ouvertes.fr/hal-03401880 https://doi.org/10.3389/fmicb.2019.01128 |
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ftccsdartic:oai:HAL:hal-03401880v1 2023-05-15T15:57:47+02:00 Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate Martenot, Claire Faury, Nicole Morga, Benjamin Degremont, Lionel Lamy, Jean-Baptiste Houssin, Maryline Renault, Tristan Laboratoire de Génétique et Pathologie des Mollusques Marins, 17390 La Tremblade, France. (LGPMM) Santé, Génétique et Microbiologie des Mollusques (IFREMER SG2M) Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) 2019-05-24 https://hal-normandie-univ.archives-ouvertes.fr/hal-03401880 https://doi.org/10.3389/fmicb.2019.01128 en eng HAL CCSD Frontiers Media info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.01128 hal-03401880 https://hal-normandie-univ.archives-ouvertes.fr/hal-03401880 doi:10.3389/fmicb.2019.01128 ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal-normandie-univ.archives-ouvertes.fr/hal-03401880 Frontiers in Microbiology, Frontiers Media, 2019, 10, ⟨10.3389/fmicb.2019.01128⟩ infection Crassostrea gigas interaction dextran sulfate OsHV-1 antiviral antibodies [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2019 ftccsdartic https://doi.org/10.3389/fmicb.2019.01128 2021-11-06T23:28:53Z International audience Viral entry mechanisms of herpesviruses constitute a highly complex process which implicates several viral glycoproteins and different receptors on the host cell surfaces. This initial infection stage was currently undescribed for Ostreid herpes virus 1 (OsHV-1), a herpesvirus infecting bivalves including the Pacific oyster, Crassostrea gigas. To identify OsHV-1 glyproteins implicated in the attachment of the virus to oyster cells, three viral putative membrane proteins, encoded by ORF 25, 41, and 72, were selected and polyclonal antibodies against these targets were used to explore first interactions between the virus and host cells. In addition, effects of dextran sulfate, a negative charged sulfated polysaccharide, were investigated on OsHV-1 infection. Effects of antiviral antibodies and dextran sulfate were evaluated by combining viral DNA and RNA detection in spat (in vivo trials) and in oyster hemolymph (in vitro trials). Results showed that viral protein encoded by ORF 25 appeared to be involved in interaction between OsHV-1 and host cells even if other proteins are likely implicated, such as proteins encoded by ORF 72 and ORF 41. Dextran sulfate at 30 μg/mL significantly reduced the spat mortality rate in the experimental conditions. Taken together, these results contribute to better understanding the pathogenesis of the viral infection, especially during the first stage of OsHV-1 infection, and open the way toward new approaches to control OsHV-1 infection in confined facilities. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Frontiers in Microbiology 10 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
infection Crassostrea gigas interaction dextran sulfate OsHV-1 antiviral antibodies [SDV]Life Sciences [q-bio] |
spellingShingle |
infection Crassostrea gigas interaction dextran sulfate OsHV-1 antiviral antibodies [SDV]Life Sciences [q-bio] Martenot, Claire Faury, Nicole Morga, Benjamin Degremont, Lionel Lamy, Jean-Baptiste Houssin, Maryline Renault, Tristan Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate |
topic_facet |
infection Crassostrea gigas interaction dextran sulfate OsHV-1 antiviral antibodies [SDV]Life Sciences [q-bio] |
description |
International audience Viral entry mechanisms of herpesviruses constitute a highly complex process which implicates several viral glycoproteins and different receptors on the host cell surfaces. This initial infection stage was currently undescribed for Ostreid herpes virus 1 (OsHV-1), a herpesvirus infecting bivalves including the Pacific oyster, Crassostrea gigas. To identify OsHV-1 glyproteins implicated in the attachment of the virus to oyster cells, three viral putative membrane proteins, encoded by ORF 25, 41, and 72, were selected and polyclonal antibodies against these targets were used to explore first interactions between the virus and host cells. In addition, effects of dextran sulfate, a negative charged sulfated polysaccharide, were investigated on OsHV-1 infection. Effects of antiviral antibodies and dextran sulfate were evaluated by combining viral DNA and RNA detection in spat (in vivo trials) and in oyster hemolymph (in vitro trials). Results showed that viral protein encoded by ORF 25 appeared to be involved in interaction between OsHV-1 and host cells even if other proteins are likely implicated, such as proteins encoded by ORF 72 and ORF 41. Dextran sulfate at 30 μg/mL significantly reduced the spat mortality rate in the experimental conditions. Taken together, these results contribute to better understanding the pathogenesis of the viral infection, especially during the first stage of OsHV-1 infection, and open the way toward new approaches to control OsHV-1 infection in confined facilities. |
author2 |
Laboratoire de Génétique et Pathologie des Mollusques Marins, 17390 La Tremblade, France. (LGPMM) Santé, Génétique et Microbiologie des Mollusques (IFREMER SG2M) Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) |
format |
Article in Journal/Newspaper |
author |
Martenot, Claire Faury, Nicole Morga, Benjamin Degremont, Lionel Lamy, Jean-Baptiste Houssin, Maryline Renault, Tristan |
author_facet |
Martenot, Claire Faury, Nicole Morga, Benjamin Degremont, Lionel Lamy, Jean-Baptiste Houssin, Maryline Renault, Tristan |
author_sort |
Martenot, Claire |
title |
Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate |
title_short |
Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate |
title_full |
Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate |
title_fullStr |
Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate |
title_full_unstemmed |
Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate |
title_sort |
exploring first interactions between ostreid herpesvirus 1 (oshv-1) and its host, crassostrea gigas: effects of specific antiviral antibodies and dextran sulfate |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-03401880 https://doi.org/10.3389/fmicb.2019.01128 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal-normandie-univ.archives-ouvertes.fr/hal-03401880 Frontiers in Microbiology, Frontiers Media, 2019, 10, ⟨10.3389/fmicb.2019.01128⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.01128 hal-03401880 https://hal-normandie-univ.archives-ouvertes.fr/hal-03401880 doi:10.3389/fmicb.2019.01128 |
op_doi |
https://doi.org/10.3389/fmicb.2019.01128 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766393472512688128 |