Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation
International audience In the dark ocean, the balance between the heterotrophic carbon demand and the supply of sinking carbon through the biological carbon pump remains poorly constrained. In situ tracking of the dynamics of microbial degradation processes occurring on the gravitational sinking par...
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Online Access: | https://hal.archives-ouvertes.fr/hal-03372797 https://doi.org/10.3390/w13192616 |
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ftccsdartic:oai:HAL:hal-03372797v1 2023-05-15T17:35:08+02:00 Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation Jacquet, Stéphanie Tamburini, Christian Garel, Marc Barani, Aude Boeuf, Dominique Bonin, Patricia Bhairy, Nagib Guasco, Sophie Le Moigne, Frédéric Panagiotopoulos, Christos Riou, Virginie Veloso, Sandrine Santinelli, Chiara Armougom, Fabrice Lefèvre, Dominique Institut méditerranéen d'océanologie (MIO) Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Toulon (UTLN) 2021-10 https://hal.archives-ouvertes.fr/hal-03372797 https://doi.org/10.3390/w13192616 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/w13192616 hal-03372797 https://hal.archives-ouvertes.fr/hal-03372797 doi:10.3390/w13192616 ISSN: 2073-4441 Water https://hal.archives-ouvertes.fr/hal-03372797 Water, MDPI, 2021, 13 (19), pp.2616. ⟨10.3390/w13192616⟩ [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere info:eu-repo/semantics/article Journal articles 2021 ftccsdartic https://doi.org/10.3390/w13192616 2021-10-16T22:23:47Z International audience In the dark ocean, the balance between the heterotrophic carbon demand and the supply of sinking carbon through the biological carbon pump remains poorly constrained. In situ tracking of the dynamics of microbial degradation processes occurring on the gravitational sinking particles is still challenging. Our particle sinking simulator system (PASS) intends to mimic as closely as possible the in situ variations in pressure and temperature experienced by gravitational sinking particles. Here, we used the PASS to simultaneously track geochemical and microbial changes that occurred during the sinking through the mesopelagic zone of laboratory-grown Emiliania huxleyi aggregates amended by a natural microbial community sampled at 105 m depth in the North Atlantic Ocean. The impact of pressure on the prokaryotic degradation of POC and dissolution of E. huxleyi-derived calcite was not marked compared to atmospheric pressure. In contrast, using global O2 consumption monitored in real-time inside the high-pressure bottles using planar optodes via a sapphire window, a reduction of respiration rate was recorded in surface-originated community assemblages under increasing pressure conditions. Moreover, using a 16S rRNA metabarcoding survey, we demonstrated a drastic difference in transcriptionally active prokaryotes associated with particles, incubated either at atmospheric pressure or under linearly increasing hydrostatic pressure conditions. The increase in hydrostatic pressure reduced both the phylogenetic diversity and the species richness. The incubation at atmospheric pressure, however, promoted an opportunistic community of “fast” degraders from the surface (Saccharospirillaceae, Hyphomonadaceae, and Pseudoalteromonadaceae), known to be associated with surface phytoplankton blooms. In contrast, the incubation under increasing pressure condition incubations revealed an increase in the particle colonizer families Flavobacteriaceae and Rhodobacteraceae, and also Colwelliaceae, which are known to ... Article in Journal/Newspaper North Atlantic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Water 13 19 2616 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere |
spellingShingle |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere Jacquet, Stéphanie Tamburini, Christian Garel, Marc Barani, Aude Boeuf, Dominique Bonin, Patricia Bhairy, Nagib Guasco, Sophie Le Moigne, Frédéric Panagiotopoulos, Christos Riou, Virginie Veloso, Sandrine Santinelli, Chiara Armougom, Fabrice Lefèvre, Dominique Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
topic_facet |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere |
description |
International audience In the dark ocean, the balance between the heterotrophic carbon demand and the supply of sinking carbon through the biological carbon pump remains poorly constrained. In situ tracking of the dynamics of microbial degradation processes occurring on the gravitational sinking particles is still challenging. Our particle sinking simulator system (PASS) intends to mimic as closely as possible the in situ variations in pressure and temperature experienced by gravitational sinking particles. Here, we used the PASS to simultaneously track geochemical and microbial changes that occurred during the sinking through the mesopelagic zone of laboratory-grown Emiliania huxleyi aggregates amended by a natural microbial community sampled at 105 m depth in the North Atlantic Ocean. The impact of pressure on the prokaryotic degradation of POC and dissolution of E. huxleyi-derived calcite was not marked compared to atmospheric pressure. In contrast, using global O2 consumption monitored in real-time inside the high-pressure bottles using planar optodes via a sapphire window, a reduction of respiration rate was recorded in surface-originated community assemblages under increasing pressure conditions. Moreover, using a 16S rRNA metabarcoding survey, we demonstrated a drastic difference in transcriptionally active prokaryotes associated with particles, incubated either at atmospheric pressure or under linearly increasing hydrostatic pressure conditions. The increase in hydrostatic pressure reduced both the phylogenetic diversity and the species richness. The incubation at atmospheric pressure, however, promoted an opportunistic community of “fast” degraders from the surface (Saccharospirillaceae, Hyphomonadaceae, and Pseudoalteromonadaceae), known to be associated with surface phytoplankton blooms. In contrast, the incubation under increasing pressure condition incubations revealed an increase in the particle colonizer families Flavobacteriaceae and Rhodobacteraceae, and also Colwelliaceae, which are known to ... |
author2 |
Institut méditerranéen d'océanologie (MIO) Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Toulon (UTLN) |
format |
Article in Journal/Newspaper |
author |
Jacquet, Stéphanie Tamburini, Christian Garel, Marc Barani, Aude Boeuf, Dominique Bonin, Patricia Bhairy, Nagib Guasco, Sophie Le Moigne, Frédéric Panagiotopoulos, Christos Riou, Virginie Veloso, Sandrine Santinelli, Chiara Armougom, Fabrice Lefèvre, Dominique |
author_facet |
Jacquet, Stéphanie Tamburini, Christian Garel, Marc Barani, Aude Boeuf, Dominique Bonin, Patricia Bhairy, Nagib Guasco, Sophie Le Moigne, Frédéric Panagiotopoulos, Christos Riou, Virginie Veloso, Sandrine Santinelli, Chiara Armougom, Fabrice Lefèvre, Dominique |
author_sort |
Jacquet, Stéphanie |
title |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_short |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_full |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_fullStr |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_full_unstemmed |
Increasing Hydrostatic Pressure Impacts the Prokaryotic Diversity during Emiliania huxleyi Aggregates Degradation |
title_sort |
increasing hydrostatic pressure impacts the prokaryotic diversity during emiliania huxleyi aggregates degradation |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.archives-ouvertes.fr/hal-03372797 https://doi.org/10.3390/w13192616 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 2073-4441 Water https://hal.archives-ouvertes.fr/hal-03372797 Water, MDPI, 2021, 13 (19), pp.2616. ⟨10.3390/w13192616⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/w13192616 hal-03372797 https://hal.archives-ouvertes.fr/hal-03372797 doi:10.3390/w13192616 |
op_doi |
https://doi.org/10.3390/w13192616 |
container_title |
Water |
container_volume |
13 |
container_issue |
19 |
container_start_page |
2616 |
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1766134190115389440 |