Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection
International audience Massive mortality outbreaks affecting Pacific oysters ( Crassostrea gigas ) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conduct...
Published in: | Frontiers in Immunology |
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Online Access: | https://hal.inrae.fr/hal-03352947 https://doi.org/10.3389/fimmu.2020.621994 |
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ftccsdartic:oai:HAL:hal-03352947v1 2023-05-15T15:59:04+02:00 Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection Leprêtre, Maxime Faury, Nicole Segarra, Amélie Claverol, Stéphane Degremont, Lionel Palos-Ladeiro, Mélissa Armengaud, J. Renault, Tristan Morga, Benjamin Médicaments et Technologies pour la Santé (MTS) Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) 2021-01-18 https://hal.inrae.fr/hal-03352947 https://doi.org/10.3389/fimmu.2020.621994 en eng HAL CCSD Frontiers info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2020.621994 info:eu-repo/semantics/altIdentifier/pmid/33537036 hal-03352947 https://hal.inrae.fr/hal-03352947 doi:10.3389/fimmu.2020.621994 PUBMED: 33537036 WOS: 000613276000001 ISSN: 1664-3224 EISSN: 1664-3224 Frontiers in Immunology https://hal.inrae.fr/hal-03352947 Frontiers in Immunology, Frontiers, 2021, 11, ⟨10.3389/fimmu.2020.621994⟩ [SDV.IMM]Life Sciences [q-bio]/Immunology info:eu-repo/semantics/article Journal articles 2021 ftccsdartic https://doi.org/10.3389/fimmu.2020.621994 2021-12-18T23:51:44Z International audience Massive mortality outbreaks affecting Pacific oysters ( Crassostrea gigas ) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and viral load observed in this oyster family. Concerning the Pacific oyster proteome, more than 3,000 proteins were identified and contrasted proteomic responses were observed between infected A- and P-oysters, sampled at different post-injection times. Gene ontology (GO) and KEGG pathway enrichment analysis performed on significantly modulated proteins uncover the main immune processes (such as RNA interference, interferon-like pathway, antioxidant defense) which contribute to the defense and resistance of Pacific oysters to viral infection. In the more susceptible Pacific oysters, results suggest that OsHV-1 manipulate the molecular machinery of host immune response, in particular the autophagy system. This immunomodulation may lead to weakening and consecutively triggering death of Pacific oysters. The identification of several highly modulated and defense-related Pacific oyster proteins from the most resistant oysters supports the crucial role played by the innate immune system against OsHV-1 and the viral infection. Our results confirm the implication of proteins involved in an interferon-like pathway for efficient antiviral defenses and suggest that proteins involved in RNA interference process prevent viral replication in C. gigas . ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Frontiers in Immunology 11 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDV.IMM]Life Sciences [q-bio]/Immunology |
spellingShingle |
[SDV.IMM]Life Sciences [q-bio]/Immunology Leprêtre, Maxime Faury, Nicole Segarra, Amélie Claverol, Stéphane Degremont, Lionel Palos-Ladeiro, Mélissa Armengaud, J. Renault, Tristan Morga, Benjamin Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
topic_facet |
[SDV.IMM]Life Sciences [q-bio]/Immunology |
description |
International audience Massive mortality outbreaks affecting Pacific oysters ( Crassostrea gigas ) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and viral load observed in this oyster family. Concerning the Pacific oyster proteome, more than 3,000 proteins were identified and contrasted proteomic responses were observed between infected A- and P-oysters, sampled at different post-injection times. Gene ontology (GO) and KEGG pathway enrichment analysis performed on significantly modulated proteins uncover the main immune processes (such as RNA interference, interferon-like pathway, antioxidant defense) which contribute to the defense and resistance of Pacific oysters to viral infection. In the more susceptible Pacific oysters, results suggest that OsHV-1 manipulate the molecular machinery of host immune response, in particular the autophagy system. This immunomodulation may lead to weakening and consecutively triggering death of Pacific oysters. The identification of several highly modulated and defense-related Pacific oyster proteins from the most resistant oysters supports the crucial role played by the innate immune system against OsHV-1 and the viral infection. Our results confirm the implication of proteins involved in an interferon-like pathway for efficient antiviral defenses and suggest that proteins involved in RNA interference process prevent viral replication in C. gigas . ... |
author2 |
Médicaments et Technologies pour la Santé (MTS) Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
format |
Article in Journal/Newspaper |
author |
Leprêtre, Maxime Faury, Nicole Segarra, Amélie Claverol, Stéphane Degremont, Lionel Palos-Ladeiro, Mélissa Armengaud, J. Renault, Tristan Morga, Benjamin |
author_facet |
Leprêtre, Maxime Faury, Nicole Segarra, Amélie Claverol, Stéphane Degremont, Lionel Palos-Ladeiro, Mélissa Armengaud, J. Renault, Tristan Morga, Benjamin |
author_sort |
Leprêtre, Maxime |
title |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_short |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_full |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_fullStr |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_full_unstemmed |
Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection |
title_sort |
comparative proteomics of ostreid herpesvirus 1 and pacific oyster interactions with two families exhibiting contrasted susceptibility to viral infection |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.inrae.fr/hal-03352947 https://doi.org/10.3389/fimmu.2020.621994 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1664-3224 EISSN: 1664-3224 Frontiers in Immunology https://hal.inrae.fr/hal-03352947 Frontiers in Immunology, Frontiers, 2021, 11, ⟨10.3389/fimmu.2020.621994⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2020.621994 info:eu-repo/semantics/altIdentifier/pmid/33537036 hal-03352947 https://hal.inrae.fr/hal-03352947 doi:10.3389/fimmu.2020.621994 PUBMED: 33537036 WOS: 000613276000001 |
op_doi |
https://doi.org/10.3389/fimmu.2020.621994 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
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1766394855947239424 |