Differentiation between populations of the Portuguese oyster, Crassostrea angulata (Lamark) and the Pacific oyster, Crassostrea gigas (Thunberg), revealed by mtDNA RFLP analysis
International audience Restriction fragment length polymorphisms of PCR-amplified mitochondrial DNA fragments were used to examine genetic differentiation between populations of the Portuguese oyster (Crassostrea angulata) and the Pacific oyster (Crassostrea gigas). The taxonomic status of C. angula...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Online Access: | https://hal.inrae.fr/hal-03327802 https://doi.org/10.1016/S0022-0981(97)00250-5 |
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ftccsdartic:oai:HAL:hal-03327802v1 2023-05-15T15:57:42+02:00 Differentiation between populations of the Portuguese oyster, Crassostrea angulata (Lamark) and the Pacific oyster, Crassostrea gigas (Thunberg), revealed by mtDNA RFLP analysis Boudry, Pierre Heurtebise, Serge Collet, Bertrand Cornette, Florence Gerard, André Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 1998 https://hal.inrae.fr/hal-03327802 https://doi.org/10.1016/S0022-0981(97)00250-5 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/S0022-0981(97)00250-5 hal-03327802 https://hal.inrae.fr/hal-03327802 doi:10.1016/S0022-0981(97)00250-5 WOS: 000074228500008 ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.inrae.fr/hal-03327802 Journal of Experimental Marine Biology and Ecology, Elsevier, 1998, 226 (2), pp.279-291. ⟨10.1016/S0022-0981(97)00250-5⟩ https://www.sciencedirect.com/journal/journal-of-experimental-marine-biology-and-ecology Crassostrea angulata Crassostrea gigas PCR mtDNA RFLP Genetic differentiation [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology info:eu-repo/semantics/article Journal articles 1998 ftccsdartic https://doi.org/10.1016/S0022-0981(97)00250-5 2021-11-20T23:50:58Z International audience Restriction fragment length polymorphisms of PCR-amplified mitochondrial DNA fragments were used to examine genetic differentiation between populations of the Portuguese oyster (Crassostrea angulata) and the Pacific oyster (Crassostrea gigas). The taxonomic status of C. angulata and C. gigas has often been questioned since no morphological or genetic differences had ever been observed between the two taxa. Samples identified as C. angulata, were collected from 16 sites located in southern Portugal and Spain and samples identified as C. gigas, from European and Asian sites. Of the six haplotypes observed, one was commonly found among oysters identified as C. gigas while another one was most frequent among oysters identified as C. angulata. Analysis of haplotype diversity among sites showed that samples originating from southern Portugal and Spain cluster with the Taiwanese sample. These results implicate Taiwan as the possible origin of European C. angulata populations. The ability to differentiate between these two previously indistinguishable taxa allowed us to identify a population in Portugal as mixed. These results open new perspectives for the study of characters previously described as varying between C. gigas and C. angulata, such as growth performance and disease susceptibility. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Lamark ENVELOPE(16.823,16.823,68.653,68.653) Pacific Journal of Experimental Marine Biology and Ecology 226 2 279 291 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Crassostrea angulata Crassostrea gigas PCR mtDNA RFLP Genetic differentiation [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology |
spellingShingle |
Crassostrea angulata Crassostrea gigas PCR mtDNA RFLP Genetic differentiation [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology Boudry, Pierre Heurtebise, Serge Collet, Bertrand Cornette, Florence Gerard, André Differentiation between populations of the Portuguese oyster, Crassostrea angulata (Lamark) and the Pacific oyster, Crassostrea gigas (Thunberg), revealed by mtDNA RFLP analysis |
topic_facet |
Crassostrea angulata Crassostrea gigas PCR mtDNA RFLP Genetic differentiation [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology |
description |
International audience Restriction fragment length polymorphisms of PCR-amplified mitochondrial DNA fragments were used to examine genetic differentiation between populations of the Portuguese oyster (Crassostrea angulata) and the Pacific oyster (Crassostrea gigas). The taxonomic status of C. angulata and C. gigas has often been questioned since no morphological or genetic differences had ever been observed between the two taxa. Samples identified as C. angulata, were collected from 16 sites located in southern Portugal and Spain and samples identified as C. gigas, from European and Asian sites. Of the six haplotypes observed, one was commonly found among oysters identified as C. gigas while another one was most frequent among oysters identified as C. angulata. Analysis of haplotype diversity among sites showed that samples originating from southern Portugal and Spain cluster with the Taiwanese sample. These results implicate Taiwan as the possible origin of European C. angulata populations. The ability to differentiate between these two previously indistinguishable taxa allowed us to identify a population in Portugal as mixed. These results open new perspectives for the study of characters previously described as varying between C. gigas and C. angulata, such as growth performance and disease susceptibility. |
author2 |
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Boudry, Pierre Heurtebise, Serge Collet, Bertrand Cornette, Florence Gerard, André |
author_facet |
Boudry, Pierre Heurtebise, Serge Collet, Bertrand Cornette, Florence Gerard, André |
author_sort |
Boudry, Pierre |
title |
Differentiation between populations of the Portuguese oyster, Crassostrea angulata (Lamark) and the Pacific oyster, Crassostrea gigas (Thunberg), revealed by mtDNA RFLP analysis |
title_short |
Differentiation between populations of the Portuguese oyster, Crassostrea angulata (Lamark) and the Pacific oyster, Crassostrea gigas (Thunberg), revealed by mtDNA RFLP analysis |
title_full |
Differentiation between populations of the Portuguese oyster, Crassostrea angulata (Lamark) and the Pacific oyster, Crassostrea gigas (Thunberg), revealed by mtDNA RFLP analysis |
title_fullStr |
Differentiation between populations of the Portuguese oyster, Crassostrea angulata (Lamark) and the Pacific oyster, Crassostrea gigas (Thunberg), revealed by mtDNA RFLP analysis |
title_full_unstemmed |
Differentiation between populations of the Portuguese oyster, Crassostrea angulata (Lamark) and the Pacific oyster, Crassostrea gigas (Thunberg), revealed by mtDNA RFLP analysis |
title_sort |
differentiation between populations of the portuguese oyster, crassostrea angulata (lamark) and the pacific oyster, crassostrea gigas (thunberg), revealed by mtdna rflp analysis |
publisher |
HAL CCSD |
publishDate |
1998 |
url |
https://hal.inrae.fr/hal-03327802 https://doi.org/10.1016/S0022-0981(97)00250-5 |
long_lat |
ENVELOPE(16.823,16.823,68.653,68.653) |
geographic |
Lamark Pacific |
geographic_facet |
Lamark Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.inrae.fr/hal-03327802 Journal of Experimental Marine Biology and Ecology, Elsevier, 1998, 226 (2), pp.279-291. ⟨10.1016/S0022-0981(97)00250-5⟩ https://www.sciencedirect.com/journal/journal-of-experimental-marine-biology-and-ecology |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0022-0981(97)00250-5 hal-03327802 https://hal.inrae.fr/hal-03327802 doi:10.1016/S0022-0981(97)00250-5 WOS: 000074228500008 |
op_doi |
https://doi.org/10.1016/S0022-0981(97)00250-5 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
226 |
container_issue |
2 |
container_start_page |
279 |
op_container_end_page |
291 |
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1766393385140092928 |