Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides
International audience The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26...
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
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Online Access: | https://hal.inrae.fr/hal-03326843 https://doi.org/10.3390/md19080452 |
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ftccsdartic:oai:HAL:hal-03326843v1 2023-05-15T15:57:58+02:00 Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides Réalis-Doyelle, Emilie Schwartz, Julie Cabau, Cédric Le Franc, Lorane Bernay, Benoit Rivière, Guillaume Klopp, Christophe Favrel, Pascal Génétique Physiologie et Systèmes d'Elevage (GenPhySE ) Ecole Nationale Vétérinaire de Toulouse (ENVT) Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse ENSAT -Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Système d'Information des GENomes des Animaux d'Elevage (SIGENAE) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA) 2021-08 https://hal.inrae.fr/hal-03326843 https://doi.org/10.3390/md19080452 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/md19080452 hal-03326843 https://hal.inrae.fr/hal-03326843 doi:10.3390/md19080452 ISSN: 1660-3397 Marine drugs https://hal.inrae.fr/hal-03326843 Marine drugs, MDPI, 2021, 19 (8), pp.452. ⟨10.3390/md19080452⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2021 ftccsdartic https://doi.org/10.3390/md19080452 2021-08-28T22:21:02Z International audience The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samples corresponding to VG at different stages of reproduction were de novo assembled to generate a specific reference transcriptome of the oyster nervous system and used to identify differentially expressed transcripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs) related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17, Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS, clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression was observed. However, 25 NPPs displayed reproduction stage-specific expression, supporting their involvement in the control of gametogenesis or associated metabolisms. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Marine Drugs 19 8 452 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
spellingShingle |
[SDV]Life Sciences [q-bio] Réalis-Doyelle, Emilie Schwartz, Julie Cabau, Cédric Le Franc, Lorane Bernay, Benoit Rivière, Guillaume Klopp, Christophe Favrel, Pascal Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
International audience The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samples corresponding to VG at different stages of reproduction were de novo assembled to generate a specific reference transcriptome of the oyster nervous system and used to identify differentially expressed transcripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs) related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17, Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS, clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression was observed. However, 25 NPPs displayed reproduction stage-specific expression, supporting their involvement in the control of gametogenesis or associated metabolisms. |
author2 |
Génétique Physiologie et Systèmes d'Elevage (GenPhySE ) Ecole Nationale Vétérinaire de Toulouse (ENVT) Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse ENSAT -Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Système d'Information des GENomes des Animaux d'Elevage (SIGENAE) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA) |
format |
Article in Journal/Newspaper |
author |
Réalis-Doyelle, Emilie Schwartz, Julie Cabau, Cédric Le Franc, Lorane Bernay, Benoit Rivière, Guillaume Klopp, Christophe Favrel, Pascal |
author_facet |
Réalis-Doyelle, Emilie Schwartz, Julie Cabau, Cédric Le Franc, Lorane Bernay, Benoit Rivière, Guillaume Klopp, Christophe Favrel, Pascal |
author_sort |
Réalis-Doyelle, Emilie |
title |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_short |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_full |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_fullStr |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_full_unstemmed |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_sort |
transcriptome profiling of the pacific oyster crassostrea gigas visceral ganglia over a reproduction cycle identifies novel regulatory peptides |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.inrae.fr/hal-03326843 https://doi.org/10.3390/md19080452 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1660-3397 Marine drugs https://hal.inrae.fr/hal-03326843 Marine drugs, MDPI, 2021, 19 (8), pp.452. ⟨10.3390/md19080452⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/md19080452 hal-03326843 https://hal.inrae.fr/hal-03326843 doi:10.3390/md19080452 |
op_doi |
https://doi.org/10.3390/md19080452 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
8 |
container_start_page |
452 |
_version_ |
1766393689809092608 |