Transcription initiation and RNA processing in the mitochondria of the red alga Chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria
The mitochondrial DNA (mt DNA) of the red alga Chondrus crispus is shown to be transcribed into two large RNA molecules. These primary transcripts are cleaved once, at the level of a tRNA, then the resulting products are processed via multiple maturation events into either mono- or poly-cistronic RN...
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Online Access: | https://hal.archives-ouvertes.fr/hal-03238103 https://doi.org/10.1006/jmbi.1998.2112 |
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ftccsdartic:oai:HAL:hal-03238103v1 2023-05-15T17:39:53+02:00 Transcription initiation and RNA processing in the mitochondria of the red alga Chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria Richard, Odile Bonnard, Geraldine Grienenberger, Jean-Michel Kloareg, Bernard Boyen, Catherine Institut de biologie moléculaire des plantes (IBMP) Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) 1998-10-30 https://hal.archives-ouvertes.fr/hal-03238103 https://doi.org/10.1006/jmbi.1998.2112 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1006/jmbi.1998.2112 hal-03238103 https://hal.archives-ouvertes.fr/hal-03238103 doi:10.1006/jmbi.1998.2112 ISSN: 0022-2836 EISSN: 1089-8638 Journal of Molecular Biology https://hal.archives-ouvertes.fr/hal-03238103 Journal of Molecular Biology, Elsevier, 1998, 283 (3), pp.549-557. ⟨10.1006/jmbi.1998.2112⟩ http://www.sciencedirect.com/science/article/pii/S002228369892112X tRNA [SDV.BV]Life Sciences [q-bio]/Vegetal Biology info:eu-repo/semantics/article Journal articles 1998 ftccsdartic https://doi.org/10.1006/jmbi.1998.2112 2021-05-29T22:23:40Z The mitochondrial DNA (mt DNA) of the red alga Chondrus crispus is shown to be transcribed into two large RNA molecules. These primary transcripts are cleaved once, at the level of a tRNA, then the resulting products are processed via multiple maturation events into either mono- or poly-cistronic RNAs. Transcripts were detected for all genes and open reading frames, except for rps11 and orf172. For both transcription units the initiation of transcription was mapped by in vitro RNA capping and primer extension experiments within inverse repeated sequences at the north pole of the molecule. Consistent with primer extension mapping, putative promoter motifs sharing significant similarities with both chicken and Xenopus mitochondrial promoters were found in the C. crispus mitochondrial genome. Altogether C. crispus mitochondrial DNA appears to be transcribed as animal mtDNA is, suggesting that transcription mechanisms in mitochondria are dependent on the overall organization of the mitochondrial genome irrespective of the eukaryotic phylogeny. Article in Journal/Newspaper North Pole Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) North Pole Journal of Molecular Biology 283 3 549 557 |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
tRNA [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
spellingShingle |
tRNA [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Richard, Odile Bonnard, Geraldine Grienenberger, Jean-Michel Kloareg, Bernard Boyen, Catherine Transcription initiation and RNA processing in the mitochondria of the red alga Chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria |
topic_facet |
tRNA [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
description |
The mitochondrial DNA (mt DNA) of the red alga Chondrus crispus is shown to be transcribed into two large RNA molecules. These primary transcripts are cleaved once, at the level of a tRNA, then the resulting products are processed via multiple maturation events into either mono- or poly-cistronic RNAs. Transcripts were detected for all genes and open reading frames, except for rps11 and orf172. For both transcription units the initiation of transcription was mapped by in vitro RNA capping and primer extension experiments within inverse repeated sequences at the north pole of the molecule. Consistent with primer extension mapping, putative promoter motifs sharing significant similarities with both chicken and Xenopus mitochondrial promoters were found in the C. crispus mitochondrial genome. Altogether C. crispus mitochondrial DNA appears to be transcribed as animal mtDNA is, suggesting that transcription mechanisms in mitochondria are dependent on the overall organization of the mitochondrial genome irrespective of the eukaryotic phylogeny. |
author2 |
Institut de biologie moléculaire des plantes (IBMP) Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Richard, Odile Bonnard, Geraldine Grienenberger, Jean-Michel Kloareg, Bernard Boyen, Catherine |
author_facet |
Richard, Odile Bonnard, Geraldine Grienenberger, Jean-Michel Kloareg, Bernard Boyen, Catherine |
author_sort |
Richard, Odile |
title |
Transcription initiation and RNA processing in the mitochondria of the red alga Chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria |
title_short |
Transcription initiation and RNA processing in the mitochondria of the red alga Chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria |
title_full |
Transcription initiation and RNA processing in the mitochondria of the red alga Chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria |
title_fullStr |
Transcription initiation and RNA processing in the mitochondria of the red alga Chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria |
title_full_unstemmed |
Transcription initiation and RNA processing in the mitochondria of the red alga Chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria |
title_sort |
transcription initiation and rna processing in the mitochondria of the red alga chondrus crispus: convergence in the evolution of transcription mechanisms in mitochondria |
publisher |
HAL CCSD |
publishDate |
1998 |
url |
https://hal.archives-ouvertes.fr/hal-03238103 https://doi.org/10.1006/jmbi.1998.2112 |
geographic |
North Pole |
geographic_facet |
North Pole |
genre |
North Pole |
genre_facet |
North Pole |
op_source |
ISSN: 0022-2836 EISSN: 1089-8638 Journal of Molecular Biology https://hal.archives-ouvertes.fr/hal-03238103 Journal of Molecular Biology, Elsevier, 1998, 283 (3), pp.549-557. ⟨10.1006/jmbi.1998.2112⟩ http://www.sciencedirect.com/science/article/pii/S002228369892112X |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1006/jmbi.1998.2112 hal-03238103 https://hal.archives-ouvertes.fr/hal-03238103 doi:10.1006/jmbi.1998.2112 |
op_doi |
https://doi.org/10.1006/jmbi.1998.2112 |
container_title |
Journal of Molecular Biology |
container_volume |
283 |
container_issue |
3 |
container_start_page |
549 |
op_container_end_page |
557 |
_version_ |
1766140646056263680 |