Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes
International audience Arctic lakes emit methane (CH 4) to the atmosphere. The magnitude of this flux could increase with permafrost thaw but might also be mitigated by microbial CH 4 oxidation. Methane oxidation in oxic water has been extensively studied, while the contribution of anaerobic oxidati...
Published in: | Science of The Total Environment |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2020
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Subjects: | |
Online Access: | https://hal-amu.archives-ouvertes.fr/hal-03151740 https://hal-amu.archives-ouvertes.fr/hal-03151740/document https://hal-amu.archives-ouvertes.fr/hal-03151740/file/23-Cabrol_2020_AOM%20siberian%20lakes.pdf https://doi.org/10.1016/j.scitotenv.2020.139588 |
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openpolar |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
spellingShingle |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology cabrol, Léa Thalasso, Frédéric Gandois, Laure Sepulveda-Jauregui, Armando Martinez-Cruz, Karla Teisserenc, Roman Tananaev, Nikita Tveit, Alexander Svenning, Mette Barret, Maialen Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes |
topic_facet |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
description |
International audience Arctic lakes emit methane (CH 4) to the atmosphere. The magnitude of this flux could increase with permafrost thaw but might also be mitigated by microbial CH 4 oxidation. Methane oxidation in oxic water has been extensively studied, while the contribution of anaerobic oxidation of methane (AOM) to CH 4 mitigation is not fully understood. We have investigated four Northern Siberian stratified lakes in an area of discontinuous permafrost nearby Igarka, Russia. Analyses of CH 4 concentrations in the water column demonstrated that 60 to 100% of upward diffusing CH 4 was oxidized in the anoxic layers of the four lakes. A combination of pmoA and mcrA gene qPCR and 16S rRNA gene metabarcoding showed that the same taxa, all within Methylomonadaceae and including the predominant genus Methylobacter as well as Crenothrix, could be the major methane-oxidizing bacteria (MOB) in the anoxic water of the four lakes. Correlation between Methylomonadaceae and OTUs within Methylotenera, Geothrix and Geobacter genera indicated that AOM might occur in an interaction between MOB, denitrifiers and iron-cycling partners. We conclude that MOB within Methylomonadaceae could have a crucial impact on CH 4 cycling in these Siberian Arctic lakes by mitigating the majority of produced CH 4 before it leaves the anoxic zone. This finding emphasizes the importance of AOM by Methylomonadaceae and extends our knowledge about CH 4 cycle in lakes, a crucial component of the global CH 4 cycle. |
author2 |
Institut méditerranéen d'océanologie (MIO) Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS) Centro de Investigacion y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV) Laboratoire Ecologie Fonctionnelle et Environnement (ECOLAB) Institut Ecologie et Environnement (INEE) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP) Météo France-Centre National d'Études Spatiales Toulouse (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées University of Magallanes (UMAG) Melnikov Permafrost Institute The Arctic University of Norway (UiT) MAEDI (Ministère des Affaires Etrangères et duDéveloppement International) and MENESR (Ministère de l'Éducationnationale, de l'Enseignement supérieur et de la Recherche) French ministries and CONICYT (Comisión Nacional de Investigación Científica yTecnológica) (Chile) forfinancial support through the ERANet-LAC jointprogramMETHANOBASE (ELAC2014_DCC-009) European Project: ELAC2014 DCC-0092,Methanobase |
format |
Article in Journal/Newspaper |
author |
cabrol, Léa Thalasso, Frédéric Gandois, Laure Sepulveda-Jauregui, Armando Martinez-Cruz, Karla Teisserenc, Roman Tananaev, Nikita Tveit, Alexander Svenning, Mette Barret, Maialen |
author_facet |
cabrol, Léa Thalasso, Frédéric Gandois, Laure Sepulveda-Jauregui, Armando Martinez-Cruz, Karla Teisserenc, Roman Tananaev, Nikita Tveit, Alexander Svenning, Mette Barret, Maialen |
author_sort |
cabrol, Léa |
title |
Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes |
title_short |
Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes |
title_full |
Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes |
title_fullStr |
Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes |
title_full_unstemmed |
Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes |
title_sort |
anaerobic oxidation of methane and associated microbiome in anoxic water of northwestern siberian lakes |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal-amu.archives-ouvertes.fr/hal-03151740 https://hal-amu.archives-ouvertes.fr/hal-03151740/document https://hal-amu.archives-ouvertes.fr/hal-03151740/file/23-Cabrol_2020_AOM%20siberian%20lakes.pdf https://doi.org/10.1016/j.scitotenv.2020.139588 |
long_lat |
ENVELOPE(-126.826,-126.826,54.858,54.858) ENVELOPE(86.603,86.603,67.466,67.466) |
geographic |
Arctic Four Lakes Igarka |
geographic_facet |
Arctic Four Lakes Igarka |
genre |
Arctic permafrost |
genre_facet |
Arctic permafrost |
op_source |
ISSN: 0048-9697 EISSN: 1879-1026 Science of the Total Environment https://hal-amu.archives-ouvertes.fr/hal-03151740 Science of the Total Environment, Elsevier, 2020, 736, pp.139588. ⟨10.1016/j.scitotenv.2020.139588⟩ |
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2020.139588 info:eu-repo/grantAgreement//ELAC2014 DCC-0092/EU/ERANET-LAC/Methanobase hal-03151740 https://hal-amu.archives-ouvertes.fr/hal-03151740 https://hal-amu.archives-ouvertes.fr/hal-03151740/document https://hal-amu.archives-ouvertes.fr/hal-03151740/file/23-Cabrol_2020_AOM%20siberian%20lakes.pdf doi:10.1016/j.scitotenv.2020.139588 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/ info:eu-repo/semantics/OpenAccess |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.scitotenv.2020.139588 |
container_title |
Science of The Total Environment |
container_volume |
736 |
container_start_page |
139588 |
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1766333010151473152 |
spelling |
ftccsdartic:oai:HAL:hal-03151740v1 2023-05-15T15:00:58+02:00 Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes cabrol, Léa Thalasso, Frédéric Gandois, Laure Sepulveda-Jauregui, Armando Martinez-Cruz, Karla Teisserenc, Roman Tananaev, Nikita Tveit, Alexander Svenning, Mette Barret, Maialen Institut méditerranéen d'océanologie (MIO) Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS) Centro de Investigacion y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV) Laboratoire Ecologie Fonctionnelle et Environnement (ECOLAB) Institut Ecologie et Environnement (INEE) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP) Météo France-Centre National d'Études Spatiales Toulouse (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées University of Magallanes (UMAG) Melnikov Permafrost Institute The Arctic University of Norway (UiT) MAEDI (Ministère des Affaires Etrangères et duDéveloppement International) and MENESR (Ministère de l'Éducationnationale, de l'Enseignement supérieur et de la Recherche) French ministries and CONICYT (Comisión Nacional de Investigación Científica yTecnológica) (Chile) forfinancial support through the ERANet-LAC jointprogramMETHANOBASE (ELAC2014_DCC-009) European Project: ELAC2014 DCC-0092,Methanobase 2020-09 https://hal-amu.archives-ouvertes.fr/hal-03151740 https://hal-amu.archives-ouvertes.fr/hal-03151740/document https://hal-amu.archives-ouvertes.fr/hal-03151740/file/23-Cabrol_2020_AOM%20siberian%20lakes.pdf https://doi.org/10.1016/j.scitotenv.2020.139588 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2020.139588 info:eu-repo/grantAgreement//ELAC2014 DCC-0092/EU/ERANET-LAC/Methanobase hal-03151740 https://hal-amu.archives-ouvertes.fr/hal-03151740 https://hal-amu.archives-ouvertes.fr/hal-03151740/document https://hal-amu.archives-ouvertes.fr/hal-03151740/file/23-Cabrol_2020_AOM%20siberian%20lakes.pdf doi:10.1016/j.scitotenv.2020.139588 http://creativecommons.org/licenses/by-nc-nd/ info:eu-repo/semantics/OpenAccess CC-BY-NC-ND ISSN: 0048-9697 EISSN: 1879-1026 Science of the Total Environment https://hal-amu.archives-ouvertes.fr/hal-03151740 Science of the Total Environment, Elsevier, 2020, 736, pp.139588. ⟨10.1016/j.scitotenv.2020.139588⟩ [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology info:eu-repo/semantics/article Journal articles 2020 ftccsdartic https://doi.org/10.1016/j.scitotenv.2020.139588 2021-11-21T00:15:43Z International audience Arctic lakes emit methane (CH 4) to the atmosphere. The magnitude of this flux could increase with permafrost thaw but might also be mitigated by microbial CH 4 oxidation. Methane oxidation in oxic water has been extensively studied, while the contribution of anaerobic oxidation of methane (AOM) to CH 4 mitigation is not fully understood. We have investigated four Northern Siberian stratified lakes in an area of discontinuous permafrost nearby Igarka, Russia. Analyses of CH 4 concentrations in the water column demonstrated that 60 to 100% of upward diffusing CH 4 was oxidized in the anoxic layers of the four lakes. A combination of pmoA and mcrA gene qPCR and 16S rRNA gene metabarcoding showed that the same taxa, all within Methylomonadaceae and including the predominant genus Methylobacter as well as Crenothrix, could be the major methane-oxidizing bacteria (MOB) in the anoxic water of the four lakes. Correlation between Methylomonadaceae and OTUs within Methylotenera, Geothrix and Geobacter genera indicated that AOM might occur in an interaction between MOB, denitrifiers and iron-cycling partners. We conclude that MOB within Methylomonadaceae could have a crucial impact on CH 4 cycling in these Siberian Arctic lakes by mitigating the majority of produced CH 4 before it leaves the anoxic zone. This finding emphasizes the importance of AOM by Methylomonadaceae and extends our knowledge about CH 4 cycle in lakes, a crucial component of the global CH 4 cycle. Article in Journal/Newspaper Arctic permafrost Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic Four Lakes ENVELOPE(-126.826,-126.826,54.858,54.858) Igarka ENVELOPE(86.603,86.603,67.466,67.466) Science of The Total Environment 736 139588 |