Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator
International audience Carbon nanofibers (CNF) are efficient electrode modifiers in electrochemical biosensors that enhance the electrochemical active area, induce electrocatalytic effect toward the oxidation of the enzymatic cofactor nicotinamide adenine dinucleotide (reduced form, NADH), and enabl...
Published in: | Microchimica Acta |
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Online Access: | https://hal.archives-ouvertes.fr/hal-03089839 https://doi.org/10.1007/s00604-020-04477-3 |
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ftccsdartic:oai:HAL:hal-03089839v1 2023-05-15T13:49:24+02:00 Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator Titoiu, Ana Maria Necula-Petrareanu, Georgiana Visinescu, Diana Dinca, Valentina Bonciu, Anca Mihailescu, Cristian Purcarea, Cristina Boukherroub, Rabah Szunerits, Sabine Vasilescu, Alina Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN) Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF) NanoBioInterfaces - IEMN (NBI - IEMN) Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF) 2020-09-05 https://hal.archives-ouvertes.fr/hal-03089839 https://doi.org/10.1007/s00604-020-04477-3 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00604-020-04477-3 hal-03089839 https://hal.archives-ouvertes.fr/hal-03089839 doi:10.1007/s00604-020-04477-3 WOS: 000565885100001 ISSN: 0026-3672 EISSN: 1436-5073 Microchimica Acta https://hal.archives-ouvertes.fr/hal-03089839 Microchimica Acta, Springer Verlag, 2020, 187 (10), ⟨10.1007/s00604-020-04477-3⟩ [CHIM.ANAL]Chemical Sciences/Analytical chemistry info:eu-repo/semantics/article Journal articles 2020 ftccsdartic https://doi.org/10.1007/s00604-020-04477-3 2021-12-19T00:37:24Z International audience Carbon nanofibers (CNF) are efficient electrode modifiers in electrochemical biosensors that enhance the electrochemical active area, induce electrocatalytic effect toward the oxidation of the enzymatic cofactor nicotinamide adenine dinucleotide (reduced form, NADH), and enable the quantitative immobilization of enzymes. Combining CNF with efficient and stable mediators radically augments the speed of electron transfer between NADH and solid electrodes and leads to electrochemical sensors characterized by high sensitivity and stability. The main aim of this work was to investigate the performance of a novel mediator for NADH with advantageously low solubility in an electrochemical detector based on a screen-printed CNF electrode as well as its potential in biosensing. Using a mediator, prepared from Meldola Blue and Ni hexamine chloride, a stable and sensitive electrochemical NADH sensor is provided with a detection limit of 0.5 μmol L−1. Further on, covalent immobilization of a recently described aldehyde dehydrogenase from the Antarctic Flavobacterium PL002 strain on the surface of the mediator-modified electrode produced a stable biosensor for the detection of aldehydes. When integrated in a flow injection analysis (FIA) setup with amperometric detection at 0.1 V vs. Ag/AgCl, the measurement of benzaldehyde with a detection limit of 10 μmol L−1 over a linear range of 30–300 μmol L−1 is possible. Determination of trace benzaldehyde impurities in a pharmaceutical excipient was also demonstrated and results compared with a chromatographic method. Article in Journal/Newspaper Antarc* Antarctic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Antarctic The Antarctic Microchimica Acta 187 10 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[CHIM.ANAL]Chemical Sciences/Analytical chemistry |
spellingShingle |
[CHIM.ANAL]Chemical Sciences/Analytical chemistry Titoiu, Ana Maria Necula-Petrareanu, Georgiana Visinescu, Diana Dinca, Valentina Bonciu, Anca Mihailescu, Cristian Purcarea, Cristina Boukherroub, Rabah Szunerits, Sabine Vasilescu, Alina Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator |
topic_facet |
[CHIM.ANAL]Chemical Sciences/Analytical chemistry |
description |
International audience Carbon nanofibers (CNF) are efficient electrode modifiers in electrochemical biosensors that enhance the electrochemical active area, induce electrocatalytic effect toward the oxidation of the enzymatic cofactor nicotinamide adenine dinucleotide (reduced form, NADH), and enable the quantitative immobilization of enzymes. Combining CNF with efficient and stable mediators radically augments the speed of electron transfer between NADH and solid electrodes and leads to electrochemical sensors characterized by high sensitivity and stability. The main aim of this work was to investigate the performance of a novel mediator for NADH with advantageously low solubility in an electrochemical detector based on a screen-printed CNF electrode as well as its potential in biosensing. Using a mediator, prepared from Meldola Blue and Ni hexamine chloride, a stable and sensitive electrochemical NADH sensor is provided with a detection limit of 0.5 μmol L−1. Further on, covalent immobilization of a recently described aldehyde dehydrogenase from the Antarctic Flavobacterium PL002 strain on the surface of the mediator-modified electrode produced a stable biosensor for the detection of aldehydes. When integrated in a flow injection analysis (FIA) setup with amperometric detection at 0.1 V vs. Ag/AgCl, the measurement of benzaldehyde with a detection limit of 10 μmol L−1 over a linear range of 30–300 μmol L−1 is possible. Determination of trace benzaldehyde impurities in a pharmaceutical excipient was also demonstrated and results compared with a chromatographic method. |
author2 |
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN) Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF) NanoBioInterfaces - IEMN (NBI - IEMN) Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF) |
format |
Article in Journal/Newspaper |
author |
Titoiu, Ana Maria Necula-Petrareanu, Georgiana Visinescu, Diana Dinca, Valentina Bonciu, Anca Mihailescu, Cristian Purcarea, Cristina Boukherroub, Rabah Szunerits, Sabine Vasilescu, Alina |
author_facet |
Titoiu, Ana Maria Necula-Petrareanu, Georgiana Visinescu, Diana Dinca, Valentina Bonciu, Anca Mihailescu, Cristian Purcarea, Cristina Boukherroub, Rabah Szunerits, Sabine Vasilescu, Alina |
author_sort |
Titoiu, Ana Maria |
title |
Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator |
title_short |
Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator |
title_full |
Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator |
title_fullStr |
Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator |
title_full_unstemmed |
Flow injection enzymatic biosensor for aldehydes based on a Meldola Blue-Ni complex electrochemical mediator |
title_sort |
flow injection enzymatic biosensor for aldehydes based on a meldola blue-ni complex electrochemical mediator |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.archives-ouvertes.fr/hal-03089839 https://doi.org/10.1007/s00604-020-04477-3 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
ISSN: 0026-3672 EISSN: 1436-5073 Microchimica Acta https://hal.archives-ouvertes.fr/hal-03089839 Microchimica Acta, Springer Verlag, 2020, 187 (10), ⟨10.1007/s00604-020-04477-3⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00604-020-04477-3 hal-03089839 https://hal.archives-ouvertes.fr/hal-03089839 doi:10.1007/s00604-020-04477-3 WOS: 000565885100001 |
op_doi |
https://doi.org/10.1007/s00604-020-04477-3 |
container_title |
Microchimica Acta |
container_volume |
187 |
container_issue |
10 |
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1766251300301832192 |