Radical Depolymerization of Alginate Extracted from Moroccan Brown Seaweed Bifurcaria bifurcata

• Published in: Applied Sciences
• Main Authors: Bouissil, Soukaina, el Alaoui-Talibi, Zainab, Pierre, Guillaume, Rchid, Halima, Michaud, Philippe, El Modafar, Cherkaoui, Delattre, Cédric
• Other Authors: Laboratoire de Biotechnologie et Bioingénierie Moléculaire, Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakesh 40000, Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Laboratoire de Biotechnologies et Valorisation des Ressources Végétales, Faculté des Sciences, Université Chouaib Doukkali, El Jadida 24000, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: Atlantic coast; hydrogen peroxide; Bifurcaria bifurcata; alginate; oligo-alginates; [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Biochemistry [q-bio.BM]; [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering; Northwest Atlantic
• Online Access: https://hal.archives-ouvertes.fr/hal-03052375; https://hal.archives-ouvertes.fr/hal-03052375/document; https://hal.archives-ouvertes.fr/hal-03052375/file/applsci-10-04166.pdf; https://doi.org/10.3390/app10124166

International audience The degradation of alginate extracted from Moroccan Bifurcaria bifurcata has not been fully established to date. In this work, we report the extraction and the characterization of alginate (ASBB) from the brown algae B. bifurcata, as well as the production of oligo-alginates (OGABs) by using a green chemistry process. The depolymerization of ASBB was carried out by controlled radical hydrolysis through our green chemistry process using a hydrogen peroxide (H2O2) catalyst. The molecular weight (Mw) and degree of polymerization (DP) distribution of oligo-alginates (OGABs) obtained were then characterized by HPLC size exclusion chromatography (SEC) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Structural characterization revealed that after 6 h of depolymerization of ASBB, we obtained OGABs with Mw ≤ 5.5 kDa and 2 ≤ DP ≤ 24. These results highlight the effectiveness of the controlled radical hydrolysis of alginate to produce good yields of alginate fractions with controlled Mw with a known polymerization degree (DP) and without altering properties of oligo-alginates. Bifurcaria bifurcata can be a potential source of alginate and oligo-alginates given its abundance on the northwest Atlantic coast. The production and characterization of oligo-alginates promote their exploitation in the cosmetic, pharmaceutic, and agriculture fields