Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay
International audience Salmonid alphavirus (SAV) is the etiological cause of pancreas disease (PD) in Atlantic salmon (Salmo salar). Several vaccines against SAV are in use, but PD still cause significant mortality and concern in European aquaculture, raising the need for optimal tools to monitor SA...
Published in: | Fish & Shellfish Immunology |
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Online Access: | https://hal.archives-ouvertes.fr/hal-03032285 https://hal.archives-ouvertes.fr/hal-03032285/document https://hal.archives-ouvertes.fr/hal-03032285/file/2020_Teige_Fish%20Shellfish%20Immunol.pdf https://doi.org/10.1016/j.fsi.2020.07.055 |
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ftccsdartic:oai:HAL:hal-03032285v1 2023-05-15T15:30:56+02:00 Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay Teige, Lena Hammerlund Aksnes, Ida Røsæg, Magnus Vikan Jensen, Ingvill Jørgensen, Jorunn B. Sindre, Hilde Collins, Catherine M. Collet, Bertrand Rimstad, Espen Dahle, Maria Krudtaa Boysen, Preben Norwegian University of Life Sciences (NMBU) Salmar Norwegian College of Fishery Science University of Tromsø (UiT) Norwegian Veterinary Institute Oslo Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Norges Miljø- og Biovitenskapelige Universitet, NMBU Norges Forskningsråd: 280847/E40, 237315/E40 European Project: 311993,EC:FP7:KBBE,FP7-KBBE-2012-6-singlestage,TARGETFISH(2012) 2020 https://hal.archives-ouvertes.fr/hal-03032285 https://hal.archives-ouvertes.fr/hal-03032285/document https://hal.archives-ouvertes.fr/hal-03032285/file/2020_Teige_Fish%20Shellfish%20Immunol.pdf https://doi.org/10.1016/j.fsi.2020.07.055 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/pmid/32738513 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2020.07.055 info:eu-repo/grantAgreement/EC/FP7/311993/EU/Targeted disease prophylaxis in European fish farming/TARGETFISH hal-03032285 https://hal.archives-ouvertes.fr/hal-03032285 https://hal.archives-ouvertes.fr/hal-03032285/document https://hal.archives-ouvertes.fr/hal-03032285/file/2020_Teige_Fish%20Shellfish%20Immunol.pdf PUBMED: 32738513 doi:10.1016/j.fsi.2020.07.055 WOS: 000579494400037 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.archives-ouvertes.fr/hal-03032285 Fish and Shellfish Immunology, Elsevier, 2020, 106, pp.374-383. ⟨10.1016/j.fsi.2020.07.055⟩ Salmonid alphavirus Bead-based assay Atlantic salmon Antibodies SAV Serological assay xMap [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2020 ftccsdartic https://doi.org/10.1016/j.fsi.2020.07.055 2021-12-19T00:42:30Z International audience Salmonid alphavirus (SAV) is the etiological cause of pancreas disease (PD) in Atlantic salmon (Salmo salar). Several vaccines against SAV are in use, but PD still cause significant mortality and concern in European aquaculture, raising the need for optimal tools to monitor SAV immunity. To monitor and control the distribution of PD in Norway, all salmonid farms are regularly screened for SAV by RT-qPCR. While the direct detection of SAV is helpful in the early stages of infection, serological methods could bring additional information on acquired SAV immunity in the later stages. Traditionally, SAV antibodies are monitored in neutralization assays, but they are time-consuming and cumbersome, thus alternative assays are warranted. Enzyme-linked immunosorbent assays (ELISAs) have not yet been successfully used for anti-SAV antibody detection in aquaculture. We aimed to develop a bead-based immunoassay for SAV-specific antibodies. By using detergent-treated SAV particles as antigens, we detected SAV-specific antibodies in plasma collected from both a SAV challenge trial and a field outbreak of PD. Increased levels of SAV-specific antibodies were seen after most fish had become negative for viral RNA. The bead-based assay is time saving compared to virus neutralization assays, and suitable for non-lethal testing due to low sample size requirements. We conclude that the bead-based immunoassay for SAV antibody detection is a promising diagnostic tool to complement SAV screening in aquaculture. Article in Journal/Newspaper Atlantic salmon Salmo salar Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Norway Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Fish & Shellfish Immunology 106 374 383 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Salmonid alphavirus Bead-based assay Atlantic salmon Antibodies SAV Serological assay xMap [SDV]Life Sciences [q-bio] |
spellingShingle |
Salmonid alphavirus Bead-based assay Atlantic salmon Antibodies SAV Serological assay xMap [SDV]Life Sciences [q-bio] Teige, Lena Hammerlund Aksnes, Ida Røsæg, Magnus Vikan Jensen, Ingvill Jørgensen, Jorunn B. Sindre, Hilde Collins, Catherine M. Collet, Bertrand Rimstad, Espen Dahle, Maria Krudtaa Boysen, Preben Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
topic_facet |
Salmonid alphavirus Bead-based assay Atlantic salmon Antibodies SAV Serological assay xMap [SDV]Life Sciences [q-bio] |
description |
International audience Salmonid alphavirus (SAV) is the etiological cause of pancreas disease (PD) in Atlantic salmon (Salmo salar). Several vaccines against SAV are in use, but PD still cause significant mortality and concern in European aquaculture, raising the need for optimal tools to monitor SAV immunity. To monitor and control the distribution of PD in Norway, all salmonid farms are regularly screened for SAV by RT-qPCR. While the direct detection of SAV is helpful in the early stages of infection, serological methods could bring additional information on acquired SAV immunity in the later stages. Traditionally, SAV antibodies are monitored in neutralization assays, but they are time-consuming and cumbersome, thus alternative assays are warranted. Enzyme-linked immunosorbent assays (ELISAs) have not yet been successfully used for anti-SAV antibody detection in aquaculture. We aimed to develop a bead-based immunoassay for SAV-specific antibodies. By using detergent-treated SAV particles as antigens, we detected SAV-specific antibodies in plasma collected from both a SAV challenge trial and a field outbreak of PD. Increased levels of SAV-specific antibodies were seen after most fish had become negative for viral RNA. The bead-based assay is time saving compared to virus neutralization assays, and suitable for non-lethal testing due to low sample size requirements. We conclude that the bead-based immunoassay for SAV antibody detection is a promising diagnostic tool to complement SAV screening in aquaculture. |
author2 |
Norwegian University of Life Sciences (NMBU) Salmar Norwegian College of Fishery Science University of Tromsø (UiT) Norwegian Veterinary Institute Oslo Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Norges Miljø- og Biovitenskapelige Universitet, NMBU Norges Forskningsråd: 280847/E40, 237315/E40 European Project: 311993,EC:FP7:KBBE,FP7-KBBE-2012-6-singlestage,TARGETFISH(2012) |
format |
Article in Journal/Newspaper |
author |
Teige, Lena Hammerlund Aksnes, Ida Røsæg, Magnus Vikan Jensen, Ingvill Jørgensen, Jorunn B. Sindre, Hilde Collins, Catherine M. Collet, Bertrand Rimstad, Espen Dahle, Maria Krudtaa Boysen, Preben |
author_facet |
Teige, Lena Hammerlund Aksnes, Ida Røsæg, Magnus Vikan Jensen, Ingvill Jørgensen, Jorunn B. Sindre, Hilde Collins, Catherine M. Collet, Bertrand Rimstad, Espen Dahle, Maria Krudtaa Boysen, Preben |
author_sort |
Teige, Lena Hammerlund |
title |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_short |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_full |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_fullStr |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_full_unstemmed |
Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
title_sort |
detection of specific atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.archives-ouvertes.fr/hal-03032285 https://hal.archives-ouvertes.fr/hal-03032285/document https://hal.archives-ouvertes.fr/hal-03032285/file/2020_Teige_Fish%20Shellfish%20Immunol.pdf https://doi.org/10.1016/j.fsi.2020.07.055 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Norway Sav’ |
geographic_facet |
Norway Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.archives-ouvertes.fr/hal-03032285 Fish and Shellfish Immunology, Elsevier, 2020, 106, pp.374-383. ⟨10.1016/j.fsi.2020.07.055⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/32738513 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2020.07.055 info:eu-repo/grantAgreement/EC/FP7/311993/EU/Targeted disease prophylaxis in European fish farming/TARGETFISH hal-03032285 https://hal.archives-ouvertes.fr/hal-03032285 https://hal.archives-ouvertes.fr/hal-03032285/document https://hal.archives-ouvertes.fr/hal-03032285/file/2020_Teige_Fish%20Shellfish%20Immunol.pdf PUBMED: 32738513 doi:10.1016/j.fsi.2020.07.055 WOS: 000579494400037 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2020.07.055 |
container_title |
Fish & Shellfish Immunology |
container_volume |
106 |
container_start_page |
374 |
op_container_end_page |
383 |
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1766361416563949568 |