Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas)
WOS:000560848100001 Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membran...
Published in: | Nanotoxicology |
---|---|
Main Authors: | , , , , , , , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2020
|
Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-03015209 https://doi.org/10.1080/17435390.2020.1808104 |
id |
ftccsdartic:oai:HAL:hal-03015209v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
ACL oyster flow-cytometry toxicity Nanoplastics paracentrotus-lividus pacific oyster reproductive success spermatozoa sperm oxidative stress fertilization embryo-larval development charged polystyrene nanoparticles motility [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL oyster flow-cytometry toxicity Nanoplastics paracentrotus-lividus pacific oyster reproductive success spermatozoa sperm oxidative stress fertilization embryo-larval development charged polystyrene nanoparticles motility [SDE.BE]Environmental Sciences/Biodiversity and Ecology Tallec, K. Paul-Pont, Ika Boulais, M. Le Goïc, Nelly Gonzalez-Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, C. Cassone, A.-L. Lambert, Christophe Soudant, Philippe Huvet, Arnaud Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas) |
topic_facet |
ACL oyster flow-cytometry toxicity Nanoplastics paracentrotus-lividus pacific oyster reproductive success spermatozoa sperm oxidative stress fertilization embryo-larval development charged polystyrene nanoparticles motility [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
WOS:000560848100001 Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 mu g mL(-1)) of 50-nm polystyrene beads with amine (50-NH(2)beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH(2)beads at 10 mu g mL(-1)induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (-79%) and in the velocity (-62%) compared to control spermatozoa, with an overall drop in embryogenesis success (-59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 mu g mL(-1)and caused a decrease in the percentage of motile spermatozoa (-66%) and in the velocity (-38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-15-CE34-0006,Nanoplastics,Microplastiques, nanoplastiques dans l'environnement marin: caractérisation, impacts et évaluation des risques sanitaires.(2015) |
format |
Article in Journal/Newspaper |
author |
Tallec, K. Paul-Pont, Ika Boulais, M. Le Goïc, Nelly Gonzalez-Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, C. Cassone, A.-L. Lambert, Christophe Soudant, Philippe Huvet, Arnaud |
author_facet |
Tallec, K. Paul-Pont, Ika Boulais, M. Le Goïc, Nelly Gonzalez-Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, C. Cassone, A.-L. Lambert, Christophe Soudant, Philippe Huvet, Arnaud |
author_sort |
Tallec, K. |
title |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas) |
title_short |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas) |
title_full |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas) |
title_fullStr |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas) |
title_full_unstemmed |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas) |
title_sort |
nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (crassostrea gigas) |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.archives-ouvertes.fr/hal-03015209 https://doi.org/10.1080/17435390.2020.1808104 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1743-5390 EISSN: 1743-5404 Nanotoxicology https://hal.archives-ouvertes.fr/hal-03015209 Nanotoxicology, Taylor & Francis, 2020, 14 (8), pp.1039-1057. ⟨10.1080/17435390.2020.1808104⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1080/17435390.2020.1808104 hal-03015209 https://hal.archives-ouvertes.fr/hal-03015209 doi:10.1080/17435390.2020.1808104 |
op_doi |
https://doi.org/10.1080/17435390.2020.1808104 |
container_title |
Nanotoxicology |
container_volume |
14 |
container_issue |
8 |
container_start_page |
1039 |
op_container_end_page |
1057 |
_version_ |
1766394735714369536 |
spelling |
ftccsdartic:oai:HAL:hal-03015209v1 2023-05-15T15:58:57+02:00 Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas) Tallec, K. Paul-Pont, Ika Boulais, M. Le Goïc, Nelly Gonzalez-Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, C. Cassone, A.-L. Lambert, Christophe Soudant, Philippe Huvet, Arnaud Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-15-CE34-0006,Nanoplastics,Microplastiques, nanoplastiques dans l'environnement marin: caractérisation, impacts et évaluation des risques sanitaires.(2015) 2020-09-13 https://hal.archives-ouvertes.fr/hal-03015209 https://doi.org/10.1080/17435390.2020.1808104 en eng HAL CCSD Taylor & Francis info:eu-repo/semantics/altIdentifier/doi/10.1080/17435390.2020.1808104 hal-03015209 https://hal.archives-ouvertes.fr/hal-03015209 doi:10.1080/17435390.2020.1808104 ISSN: 1743-5390 EISSN: 1743-5404 Nanotoxicology https://hal.archives-ouvertes.fr/hal-03015209 Nanotoxicology, Taylor & Francis, 2020, 14 (8), pp.1039-1057. ⟨10.1080/17435390.2020.1808104⟩ ACL oyster flow-cytometry toxicity Nanoplastics paracentrotus-lividus pacific oyster reproductive success spermatozoa sperm oxidative stress fertilization embryo-larval development charged polystyrene nanoparticles motility [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2020 ftccsdartic https://doi.org/10.1080/17435390.2020.1808104 2021-12-19T00:45:26Z WOS:000560848100001 Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 mu g mL(-1)) of 50-nm polystyrene beads with amine (50-NH(2)beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH(2)beads at 10 mu g mL(-1)induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (-79%) and in the velocity (-62%) compared to control spermatozoa, with an overall drop in embryogenesis success (-59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 mu g mL(-1)and caused a decrease in the percentage of motile spermatozoa (-66%) and in the velocity (-38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Nanotoxicology 14 8 1039 1057 |