The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets
International audience Big defensins, ancestors of -defensins, are composed of a -defensin-like C-terminal domain and a globular hydrophobic ancestral N-terminal domain. This unique structure is found in a limited number of phylogenetically distant species , including mollusks, ancestral chelicera...
Published in: | mBio |
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Main Authors: | , , , , , , , , , , , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2019
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Online Access: | https://hal.archives-ouvertes.fr/hal-02994659 https://hal.archives-ouvertes.fr/hal-02994659/document https://hal.archives-ouvertes.fr/hal-02994659/file/mBio-2019-Loth-e01821-19.full%20copie.pdf https://doi.org/10.1128/mBio.01821-19 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
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English |
topic |
[SDV]Life Sciences [q-bio] |
spellingShingle |
[SDV]Life Sciences [q-bio] Loth, Karine Vergnes, Agnès Barreto, Cairé Voisin, Sébastien Meudal, Herve Da Silva, Jennifer Bressan, Albert Belmadi, Nawal Bachère, Evelyne Aucagne, Vincent Cazevielle, Chantal Marchandin, Hélène Rosa, Rafael Diego Bulet, Philippe Touqui, Lhousseine DELMAS, Agnès, Destoumieux-Garzon, Delphine The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
International audience Big defensins, ancestors of -defensins, are composed of a -defensin-like C-terminal domain and a globular hydrophobic ancestral N-terminal domain. This unique structure is found in a limited number of phylogenetically distant species , including mollusks, ancestral chelicerates, and early-branching cephalochor-dates, mostly living in marine environments. One puzzling evolutionary issue concerns the advantage for these species of having maintained a hydrophobic domain lost during evolution toward -defensins. Using native ligation chemistry, we produced the oyster Crassostrea gigas BigDef1 (Cg-BigDef1) and its separate domains. Cg-BigDef1 showed salt-stable and broad-range bactericidal activity, including against multidrug-resistant human clinical isolates of Staphylococcus aureus. We found that the ancestral N-terminal domain confers salt-stable antimicrobial activity to the -defensin-like domain, which is otherwise inactive. Moreover, upon contact with bacteria, the N-terminal domain drives Cg-BigDef1 assembly into nanonets that entrap and kill bacteria. We speculate that the hydrophobic N-terminal domain of big defensins has been retained in marine phyla to confer salt-stable interactions with bacterial membranes in environments where electrostatic interactions are impaired. Those remarkable properties open the way to future drug developments when physiological salt concentrations inhibit the antimicrobial activity of vertebrate -defensins. IMPORTANCE -Defensins are host defense peptides controlling infections in species ranging from humans to invertebrates. However, the antimicrobial activity of most human -defensins is impaired at physiological salt concentrations. We explored the properties of big defensins, the -defensin ancestors, which have been conserved in a number of marine organisms, mainly mollusks. By focusing on a big defensin from oyster (Cg-BigDef1), we showed that the N-terminal domain lost during evolution toward -defensins confers bactericidal activity to ... |
author2 |
Centre de biophysique moléculaire (CBM) Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Orléans (UO) Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB) Centre Hospitalier Universitaire Grenoble (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA) |
format |
Article in Journal/Newspaper |
author |
Loth, Karine Vergnes, Agnès Barreto, Cairé Voisin, Sébastien Meudal, Herve Da Silva, Jennifer Bressan, Albert Belmadi, Nawal Bachère, Evelyne Aucagne, Vincent Cazevielle, Chantal Marchandin, Hélène Rosa, Rafael Diego Bulet, Philippe Touqui, Lhousseine DELMAS, Agnès, Destoumieux-Garzon, Delphine |
author_facet |
Loth, Karine Vergnes, Agnès Barreto, Cairé Voisin, Sébastien Meudal, Herve Da Silva, Jennifer Bressan, Albert Belmadi, Nawal Bachère, Evelyne Aucagne, Vincent Cazevielle, Chantal Marchandin, Hélène Rosa, Rafael Diego Bulet, Philippe Touqui, Lhousseine DELMAS, Agnès, Destoumieux-Garzon, Delphine |
author_sort |
Loth, Karine |
title |
The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets |
title_short |
The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets |
title_full |
The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets |
title_fullStr |
The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets |
title_full_unstemmed |
The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets |
title_sort |
ancestral n-terminal domain of big defensins drives bacterially triggered assembly into antimicrobial nanonets |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.archives-ouvertes.fr/hal-02994659 https://hal.archives-ouvertes.fr/hal-02994659/document https://hal.archives-ouvertes.fr/hal-02994659/file/mBio-2019-Loth-e01821-19.full%20copie.pdf https://doi.org/10.1128/mBio.01821-19 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 2161-2129 EISSN: 2150-7511 mBio https://hal.archives-ouvertes.fr/hal-02994659 mBio, American Society for Microbiology, 2019, 10 (5), ⟨10.1128/mBio.01821-19⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1128/mBio.01821-19 hal-02994659 https://hal.archives-ouvertes.fr/hal-02994659 https://hal.archives-ouvertes.fr/hal-02994659/document https://hal.archives-ouvertes.fr/hal-02994659/file/mBio-2019-Loth-e01821-19.full%20copie.pdf doi:10.1128/mBio.01821-19 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1128/mBio.01821-19 |
container_title |
mBio |
container_volume |
10 |
container_issue |
5 |
_version_ |
1766394718543937536 |
spelling |
ftccsdartic:oai:HAL:hal-02994659v1 2023-05-15T15:58:56+02:00 The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets Loth, Karine Vergnes, Agnès Barreto, Cairé Voisin, Sébastien Meudal, Herve Da Silva, Jennifer Bressan, Albert Belmadi, Nawal Bachère, Evelyne Aucagne, Vincent Cazevielle, Chantal Marchandin, Hélène Rosa, Rafael Diego Bulet, Philippe Touqui, Lhousseine DELMAS, Agnès, Destoumieux-Garzon, Delphine Centre de biophysique moléculaire (CBM) Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Orléans (UO) Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB) Centre Hospitalier Universitaire Grenoble (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA) 2019-10-29 https://hal.archives-ouvertes.fr/hal-02994659 https://hal.archives-ouvertes.fr/hal-02994659/document https://hal.archives-ouvertes.fr/hal-02994659/file/mBio-2019-Loth-e01821-19.full%20copie.pdf https://doi.org/10.1128/mBio.01821-19 en eng HAL CCSD American Society for Microbiology info:eu-repo/semantics/altIdentifier/doi/10.1128/mBio.01821-19 hal-02994659 https://hal.archives-ouvertes.fr/hal-02994659 https://hal.archives-ouvertes.fr/hal-02994659/document https://hal.archives-ouvertes.fr/hal-02994659/file/mBio-2019-Loth-e01821-19.full%20copie.pdf doi:10.1128/mBio.01821-19 info:eu-repo/semantics/OpenAccess ISSN: 2161-2129 EISSN: 2150-7511 mBio https://hal.archives-ouvertes.fr/hal-02994659 mBio, American Society for Microbiology, 2019, 10 (5), ⟨10.1128/mBio.01821-19⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2019 ftccsdartic https://doi.org/10.1128/mBio.01821-19 2021-11-28T00:04:57Z International audience Big defensins, ancestors of -defensins, are composed of a -defensin-like C-terminal domain and a globular hydrophobic ancestral N-terminal domain. This unique structure is found in a limited number of phylogenetically distant species , including mollusks, ancestral chelicerates, and early-branching cephalochor-dates, mostly living in marine environments. One puzzling evolutionary issue concerns the advantage for these species of having maintained a hydrophobic domain lost during evolution toward -defensins. Using native ligation chemistry, we produced the oyster Crassostrea gigas BigDef1 (Cg-BigDef1) and its separate domains. Cg-BigDef1 showed salt-stable and broad-range bactericidal activity, including against multidrug-resistant human clinical isolates of Staphylococcus aureus. We found that the ancestral N-terminal domain confers salt-stable antimicrobial activity to the -defensin-like domain, which is otherwise inactive. Moreover, upon contact with bacteria, the N-terminal domain drives Cg-BigDef1 assembly into nanonets that entrap and kill bacteria. We speculate that the hydrophobic N-terminal domain of big defensins has been retained in marine phyla to confer salt-stable interactions with bacterial membranes in environments where electrostatic interactions are impaired. Those remarkable properties open the way to future drug developments when physiological salt concentrations inhibit the antimicrobial activity of vertebrate -defensins. IMPORTANCE -Defensins are host defense peptides controlling infections in species ranging from humans to invertebrates. However, the antimicrobial activity of most human -defensins is impaired at physiological salt concentrations. We explored the properties of big defensins, the -defensin ancestors, which have been conserved in a number of marine organisms, mainly mollusks. By focusing on a big defensin from oyster (Cg-BigDef1), we showed that the N-terminal domain lost during evolution toward -defensins confers bactericidal activity to ... Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) mBio 10 5 |