Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale
WOS:000531794600001 Ostreid herpesvirus-1 (OsHV-1) is known to associate with particles in seawater, leading to infection and disease in the Pacific oyster Crassostrea gigas. The estuarine environment is highly complex and changeable, and this needs to be considered when collecting environmental sam...
Published in: | Diseases of Aquatic Organisms |
---|---|
Main Authors: | , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2020
|
Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-02987364 https://doi.org/10.3354/dao03445 |
id |
ftccsdartic:oai:HAL:hal-02987364v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:hal-02987364v1 2023-05-15T15:57:50+02:00 Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale Liu, Olivia Paul-Pont, Ika Rubio, Ana Dhand, Navneet Whittington, Richard J. The University of Sydney Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Hornsby shire council 2020 https://hal.archives-ouvertes.fr/hal-02987364 https://doi.org/10.3354/dao03445 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.3354/dao03445 hal-02987364 https://hal.archives-ouvertes.fr/hal-02987364 doi:10.3354/dao03445 Diseases of Aquatic Organisms https://hal.archives-ouvertes.fr/hal-02987364 Diseases of Aquatic Organisms, 2020, 138, pp.1--15. ⟨10.3354/dao03445⟩ ACL Crassostrea gigas Seawater Disease dna oyster crassostrea-gigas mortality river estuary panorama CNRS virus husbandry practices ostreid-herpesvirus-1 oshv-1 pacific oysters Plankton microvariant Detection new-south-wales Ostreid herpesvirus-1 [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2020 ftccsdartic https://doi.org/10.3354/dao03445 2021-12-19T00:49:22Z WOS:000531794600001 Ostreid herpesvirus-1 (OsHV-1) is known to associate with particles in seawater, leading to infection and disease in the Pacific oyster Crassostrea gigas. The estuarine environment is highly complex and changeable, and this needs to be considered when collecting environmental samples for pathogen detection. The aims of this study were to (1) compare different aspects of collecting natural seawater and plankton samples for detection of OsHV-1 DNA and (2) determine whether detection of OsHV-1 DNA in such environmental samples has merit for disease risk prediction. The results of one experiment suggest that sampling on the outgoing tide may improve the detection of OsHV-1 DNA in seawater and plankton tow samples (odds ratio 2.71). This statistical comparison was not possible in 2 other experiments. The method (plankton tow or beta bottle) and depth of collection (range: 250-1250 mm) had no effect on the likelihood of detection of OsHV-1. OsHV-1 DNA was found at low concentrations in plankton tow and seawater samples, and only when outbreaks of mortality associated with OsHV-1 were observed in nearby experimental or farmed populations of C. gigas. This suggests that single point in time environmental samples of seawater or plankton are not sufficient to rule out the presence of OsHV-1 in an estuary. The association of OsHV-1 with particles in seawater needs to be better understood in order to determine whether more selective and sensitive methods can be devised to detect it, before environmental samples can be reliably used in disease risk prediction. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Diseases of Aquatic Organisms 138 1 15 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
ACL Crassostrea gigas Seawater Disease dna oyster crassostrea-gigas mortality river estuary panorama CNRS virus husbandry practices ostreid-herpesvirus-1 oshv-1 pacific oysters Plankton microvariant Detection new-south-wales Ostreid herpesvirus-1 [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL Crassostrea gigas Seawater Disease dna oyster crassostrea-gigas mortality river estuary panorama CNRS virus husbandry practices ostreid-herpesvirus-1 oshv-1 pacific oysters Plankton microvariant Detection new-south-wales Ostreid herpesvirus-1 [SDE.BE]Environmental Sciences/Biodiversity and Ecology Liu, Olivia Paul-Pont, Ika Rubio, Ana Dhand, Navneet Whittington, Richard J. Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale |
topic_facet |
ACL Crassostrea gigas Seawater Disease dna oyster crassostrea-gigas mortality river estuary panorama CNRS virus husbandry practices ostreid-herpesvirus-1 oshv-1 pacific oysters Plankton microvariant Detection new-south-wales Ostreid herpesvirus-1 [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
WOS:000531794600001 Ostreid herpesvirus-1 (OsHV-1) is known to associate with particles in seawater, leading to infection and disease in the Pacific oyster Crassostrea gigas. The estuarine environment is highly complex and changeable, and this needs to be considered when collecting environmental samples for pathogen detection. The aims of this study were to (1) compare different aspects of collecting natural seawater and plankton samples for detection of OsHV-1 DNA and (2) determine whether detection of OsHV-1 DNA in such environmental samples has merit for disease risk prediction. The results of one experiment suggest that sampling on the outgoing tide may improve the detection of OsHV-1 DNA in seawater and plankton tow samples (odds ratio 2.71). This statistical comparison was not possible in 2 other experiments. The method (plankton tow or beta bottle) and depth of collection (range: 250-1250 mm) had no effect on the likelihood of detection of OsHV-1. OsHV-1 DNA was found at low concentrations in plankton tow and seawater samples, and only when outbreaks of mortality associated with OsHV-1 were observed in nearby experimental or farmed populations of C. gigas. This suggests that single point in time environmental samples of seawater or plankton are not sufficient to rule out the presence of OsHV-1 in an estuary. The association of OsHV-1 with particles in seawater needs to be better understood in order to determine whether more selective and sensitive methods can be devised to detect it, before environmental samples can be reliably used in disease risk prediction. |
author2 |
The University of Sydney Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Hornsby shire council |
format |
Article in Journal/Newspaper |
author |
Liu, Olivia Paul-Pont, Ika Rubio, Ana Dhand, Navneet Whittington, Richard J. |
author_facet |
Liu, Olivia Paul-Pont, Ika Rubio, Ana Dhand, Navneet Whittington, Richard J. |
author_sort |
Liu, Olivia |
title |
Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale |
title_short |
Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale |
title_full |
Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale |
title_fullStr |
Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale |
title_full_unstemmed |
Detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale |
title_sort |
detection of ostreid herpesvirus-1 in plankton and seawater samples at an estuary scale |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.archives-ouvertes.fr/hal-02987364 https://doi.org/10.3354/dao03445 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Diseases of Aquatic Organisms https://hal.archives-ouvertes.fr/hal-02987364 Diseases of Aquatic Organisms, 2020, 138, pp.1--15. ⟨10.3354/dao03445⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3354/dao03445 hal-02987364 https://hal.archives-ouvertes.fr/hal-02987364 doi:10.3354/dao03445 |
op_doi |
https://doi.org/10.3354/dao03445 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
138 |
container_start_page |
1 |
op_container_end_page |
15 |
_version_ |
1766393545871065088 |