Major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the Southern Ocean
International audience One of the first comparisons of a natural iron fertilized bloom with a high‐nutrient low‐chlorophyll (HNLC) site was undertaken during the Kerguelen ocean and plateau compared study (KEOPS) cruise. To understand better the bacteria–phytoplankton relationship in the context of...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2008
|
Subjects: | |
Online Access: | https://hal.inrae.fr/hal-02929344 https://doi.org/10.1111/j.1462-2920.2007.01497.x |
id |
ftccsdartic:oai:HAL:hal-02929344v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:hal-02929344v1 2023-05-15T18:25:10+02:00 Major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the Southern Ocean West, Nyree Obernosterer, Ingrid Zemb, Olivier Lebaron, Philippe Laboratoire d'Océanographie Microbienne (LOMIC) Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'océanographie biologique de Banyuls (LOBB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) 2008 https://hal.inrae.fr/hal-02929344 https://doi.org/10.1111/j.1462-2920.2007.01497.x en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1462-2920.2007.01497.x info:eu-repo/semantics/altIdentifier/pmid/18237307 hal-02929344 https://hal.inrae.fr/hal-02929344 doi:10.1111/j.1462-2920.2007.01497.x PUBMED: 18237307 WOS: 000252712300018 ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.inrae.fr/hal-02929344 Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2008, 10 (3), pp.738-756. ⟨10.1111/j.1462-2920.2007.01497.x⟩ phytoplankton Southern Ocean [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2008 ftccsdartic https://doi.org/10.1111/j.1462-2920.2007.01497.x 2021-11-21T00:38:50Z International audience One of the first comparisons of a natural iron fertilized bloom with a high‐nutrient low‐chlorophyll (HNLC) site was undertaken during the Kerguelen ocean and plateau compared study (KEOPS) cruise. To understand better the bacteria–phytoplankton relationship in the context of natural iron fertilization, bacterial diversity and activity was investigated in the bloom and in the adjacent HNLC region by 16S rDNA clone libraries and by single strand conformation polymorphism (SSCP) analysis. Both libraries were dominated by Alphaproteobacteria, Gammaproteobacteria and the Cytophaga‐Flavobacteria‐Bacteroides group. Cluster analysis at 99% sequence similarity yielded several microdiverse clusters and revealed striking differences between the two libraries. In the bloom, the dominant operational taxonomic units (OTUs) were the Roseobacter NAC11‐7 cluster, SAR92 and a Cytophaga‐Flavobacteria‐Bacteroides cluster related to the agg58 group, whereas in the HNLC region, SAR11, Roseobacter RCA and Polaribacter dominated. SSCP analysis of 16S rDNA and 16S rRNA revealed contrasting dynamics of three different Roseobacter OTUs. Roseobacter NAC11‐7 and NAC11‐6 had higher relative abundances and activities in the bloom compared with the HNLC site and NAC11‐6 was only detected at the decline of the bloom concomitant with a shift in phytoplankton composi tion. In contrast, Roseobacter RCA was relatively abundant and active both inside and outside of the bloom. These results suggest that the different OTUs within the Roseobacter group represent functional groups that each play an important role in the cycling of carbon. Article in Journal/Newspaper Southern Ocean Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Kerguelen Southern Ocean Environmental Microbiology 10 3 738 756 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
phytoplankton Southern Ocean [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
phytoplankton Southern Ocean [SDE.BE]Environmental Sciences/Biodiversity and Ecology West, Nyree Obernosterer, Ingrid Zemb, Olivier Lebaron, Philippe Major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the Southern Ocean |
topic_facet |
phytoplankton Southern Ocean [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience One of the first comparisons of a natural iron fertilized bloom with a high‐nutrient low‐chlorophyll (HNLC) site was undertaken during the Kerguelen ocean and plateau compared study (KEOPS) cruise. To understand better the bacteria–phytoplankton relationship in the context of natural iron fertilization, bacterial diversity and activity was investigated in the bloom and in the adjacent HNLC region by 16S rDNA clone libraries and by single strand conformation polymorphism (SSCP) analysis. Both libraries were dominated by Alphaproteobacteria, Gammaproteobacteria and the Cytophaga‐Flavobacteria‐Bacteroides group. Cluster analysis at 99% sequence similarity yielded several microdiverse clusters and revealed striking differences between the two libraries. In the bloom, the dominant operational taxonomic units (OTUs) were the Roseobacter NAC11‐7 cluster, SAR92 and a Cytophaga‐Flavobacteria‐Bacteroides cluster related to the agg58 group, whereas in the HNLC region, SAR11, Roseobacter RCA and Polaribacter dominated. SSCP analysis of 16S rDNA and 16S rRNA revealed contrasting dynamics of three different Roseobacter OTUs. Roseobacter NAC11‐7 and NAC11‐6 had higher relative abundances and activities in the bloom compared with the HNLC site and NAC11‐6 was only detected at the decline of the bloom concomitant with a shift in phytoplankton composi tion. In contrast, Roseobacter RCA was relatively abundant and active both inside and outside of the bloom. These results suggest that the different OTUs within the Roseobacter group represent functional groups that each play an important role in the cycling of carbon. |
author2 |
Laboratoire d'Océanographie Microbienne (LOMIC) Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'océanographie biologique de Banyuls (LOBB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
West, Nyree Obernosterer, Ingrid Zemb, Olivier Lebaron, Philippe |
author_facet |
West, Nyree Obernosterer, Ingrid Zemb, Olivier Lebaron, Philippe |
author_sort |
West, Nyree |
title |
Major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the Southern Ocean |
title_short |
Major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the Southern Ocean |
title_full |
Major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the Southern Ocean |
title_fullStr |
Major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the Southern Ocean |
title_full_unstemmed |
Major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the Southern Ocean |
title_sort |
major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the southern ocean |
publisher |
HAL CCSD |
publishDate |
2008 |
url |
https://hal.inrae.fr/hal-02929344 https://doi.org/10.1111/j.1462-2920.2007.01497.x |
geographic |
Kerguelen Southern Ocean |
geographic_facet |
Kerguelen Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.inrae.fr/hal-02929344 Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2008, 10 (3), pp.738-756. ⟨10.1111/j.1462-2920.2007.01497.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1462-2920.2007.01497.x info:eu-repo/semantics/altIdentifier/pmid/18237307 hal-02929344 https://hal.inrae.fr/hal-02929344 doi:10.1111/j.1462-2920.2007.01497.x PUBMED: 18237307 WOS: 000252712300018 |
op_doi |
https://doi.org/10.1111/j.1462-2920.2007.01497.x |
container_title |
Environmental Microbiology |
container_volume |
10 |
container_issue |
3 |
container_start_page |
738 |
op_container_end_page |
756 |
_version_ |
1766206407139393536 |