Diurnal expression of SAR11 proteorhodopsin and 16S rRNA genes in coastal North Atlantic waters
International audience Proteorhodopsins (PR) are phylogenetically widespread and highly expressed proton pumps in marine bacterial communities, including in the SAR11 clade, one of the most common clades in the oceans. The relationships between PR expression, light and cell activity remain un- clear...
Published in: | Aquatic Microbial Ecology |
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Online Access: | https://hal.sorbonne-universite.fr/hal-02906988 https://hal.sorbonne-universite.fr/hal-02906988/document https://hal.sorbonne-universite.fr/hal-02906988/file/a073p185.pdf https://doi.org/10.3354/ame01716 |
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ftccsdartic:oai:HAL:hal-02906988v1 2023-05-15T17:34:04+02:00 Diurnal expression of SAR11 proteorhodopsin and 16S rRNA genes in coastal North Atlantic waters Lami, Raphaël Kirchman, David L. Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM) PIERRE FABRE-EDF (EDF)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) School of Marine Science and Policy College of Earth, Ocean, and Environment Newark (CEOE) University of Delaware Newark -University of Delaware Newark 2014 https://hal.sorbonne-universite.fr/hal-02906988 https://hal.sorbonne-universite.fr/hal-02906988/document https://hal.sorbonne-universite.fr/hal-02906988/file/a073p185.pdf https://doi.org/10.3354/ame01716 en eng HAL CCSD Inter Research info:eu-repo/semantics/altIdentifier/doi/10.3354/ame01716 hal-02906988 https://hal.sorbonne-universite.fr/hal-02906988 https://hal.sorbonne-universite.fr/hal-02906988/document https://hal.sorbonne-universite.fr/hal-02906988/file/a073p185.pdf doi:10.3354/ame01716 info:eu-repo/semantics/OpenAccess ISSN: 0948-3055 Aquatic Microbial Ecology https://hal.sorbonne-universite.fr/hal-02906988 Aquatic Microbial Ecology, Inter Research, 2014, 73 (3), pp.185-194. ⟨10.3354/ame01716⟩ Marine bacteria SAR11 Proteorhodopsin Quantitative PCR [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2014 ftccsdartic https://doi.org/10.3354/ame01716 2021-12-05T01:09:32Z International audience Proteorhodopsins (PR) are phylogenetically widespread and highly expressed proton pumps in marine bacterial communities, including in the SAR11 clade, one of the most common clades in the oceans. The relationships between PR expression, light and cell activity remain un- clear, especially in natural environments. We examined these relationships during 3 diurnal stud- ies in spring, summer and fall in Delaware coastal waters. The abundance of genes and transcripts of SAR11 PR, SAR11 16S rRNA and total bacterial 16S rRNA were monitored using a quantitative PCR approach. We found that the expression of SAR11 PR was 2.5-fold higher during the day than at night. However, SAR11 16S rRNA levels remained constant during the day and night on all cruises, suggesting that the growth-related activity of SAR11 was not directly affected by sunlight. There was a tight correlation between expression of PR in SAR11 and photosynthetically active radiation, but not with other environmental parameters. Our data support the hypothesis that light affects PR expression by SAR11 populations, but the energy from PR appears to contribute relatively little to supporting bacterial growth-related activity in marine waters. Article in Journal/Newspaper North Atlantic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Aquatic Microbial Ecology 73 3 185 194 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Marine bacteria SAR11 Proteorhodopsin Quantitative PCR [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
Marine bacteria SAR11 Proteorhodopsin Quantitative PCR [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Lami, Raphaël Kirchman, David L. Diurnal expression of SAR11 proteorhodopsin and 16S rRNA genes in coastal North Atlantic waters |
topic_facet |
Marine bacteria SAR11 Proteorhodopsin Quantitative PCR [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience Proteorhodopsins (PR) are phylogenetically widespread and highly expressed proton pumps in marine bacterial communities, including in the SAR11 clade, one of the most common clades in the oceans. The relationships between PR expression, light and cell activity remain un- clear, especially in natural environments. We examined these relationships during 3 diurnal stud- ies in spring, summer and fall in Delaware coastal waters. The abundance of genes and transcripts of SAR11 PR, SAR11 16S rRNA and total bacterial 16S rRNA were monitored using a quantitative PCR approach. We found that the expression of SAR11 PR was 2.5-fold higher during the day than at night. However, SAR11 16S rRNA levels remained constant during the day and night on all cruises, suggesting that the growth-related activity of SAR11 was not directly affected by sunlight. There was a tight correlation between expression of PR in SAR11 and photosynthetically active radiation, but not with other environmental parameters. Our data support the hypothesis that light affects PR expression by SAR11 populations, but the energy from PR appears to contribute relatively little to supporting bacterial growth-related activity in marine waters. |
author2 |
Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM) PIERRE FABRE-EDF (EDF)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) School of Marine Science and Policy College of Earth, Ocean, and Environment Newark (CEOE) University of Delaware Newark -University of Delaware Newark |
format |
Article in Journal/Newspaper |
author |
Lami, Raphaël Kirchman, David L. |
author_facet |
Lami, Raphaël Kirchman, David L. |
author_sort |
Lami, Raphaël |
title |
Diurnal expression of SAR11 proteorhodopsin and 16S rRNA genes in coastal North Atlantic waters |
title_short |
Diurnal expression of SAR11 proteorhodopsin and 16S rRNA genes in coastal North Atlantic waters |
title_full |
Diurnal expression of SAR11 proteorhodopsin and 16S rRNA genes in coastal North Atlantic waters |
title_fullStr |
Diurnal expression of SAR11 proteorhodopsin and 16S rRNA genes in coastal North Atlantic waters |
title_full_unstemmed |
Diurnal expression of SAR11 proteorhodopsin and 16S rRNA genes in coastal North Atlantic waters |
title_sort |
diurnal expression of sar11 proteorhodopsin and 16s rrna genes in coastal north atlantic waters |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.sorbonne-universite.fr/hal-02906988 https://hal.sorbonne-universite.fr/hal-02906988/document https://hal.sorbonne-universite.fr/hal-02906988/file/a073p185.pdf https://doi.org/10.3354/ame01716 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 0948-3055 Aquatic Microbial Ecology https://hal.sorbonne-universite.fr/hal-02906988 Aquatic Microbial Ecology, Inter Research, 2014, 73 (3), pp.185-194. ⟨10.3354/ame01716⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3354/ame01716 hal-02906988 https://hal.sorbonne-universite.fr/hal-02906988 https://hal.sorbonne-universite.fr/hal-02906988/document https://hal.sorbonne-universite.fr/hal-02906988/file/a073p185.pdf doi:10.3354/ame01716 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3354/ame01716 |
container_title |
Aquatic Microbial Ecology |
container_volume |
73 |
container_issue |
3 |
container_start_page |
185 |
op_container_end_page |
194 |
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1766132784000139264 |