Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas
International audience This study investigated the effects on the physiology of Pacific oyster, Crassostrea gigas, of a mixture of pesticides containing 0.8 μg L-1 alachlor, 0.6 μg L-1 metolachlor, 0.7 μg L-1 atrazine, 0.6 μg L-1 terbuthylazine, 0.5 μg L-1 diuron, 0.6 μg L-1 fosetyl aluminum, 0.05 μ...
Published in: | Environmental Toxicology |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2013
|
Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-02865179 https://doi.org/10.1002/tox.20764 |
id |
ftccsdartic:oai:HAL:hal-02865179v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:hal-02865179v1 2023-05-15T15:58:20+02:00 Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas Geret, F. Burgeot, T. Haure, J. Gagnaire, B. Renault, T. Communal, P.Y. Samain, J.F. Géographie de l'environnement (GEODE) Université Toulouse - Jean Jaurès (UT2J)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Radioécologie et d'Ecotoxicologie (DEI/SECRE/LRE) Institut de Radioprotection et de Sûreté Nucléaire (IRSN) University of Angers Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2013-12-01 https://hal.archives-ouvertes.fr/hal-02865179 https://doi.org/10.1002/tox.20764 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1002/tox.20764 hal-02865179 https://hal.archives-ouvertes.fr/hal-02865179 doi:10.1002/tox.20764 ISSN: 1520-4081 Environmental Toxicology https://hal.archives-ouvertes.fr/hal-02865179 Environmental Toxicology, 2013, 28 (12), pp.689-699. ⟨10.1002/tox.20764⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.1002/tox.20764 2021-04-03T22:33:08Z International audience This study investigated the effects on the physiology of Pacific oyster, Crassostrea gigas, of a mixture of pesticides containing 0.8 μg L-1 alachlor, 0.6 μg L-1 metolachlor, 0.7 μg L-1 atrazine, 0.6 μg L-1 terbuthylazine, 0.5 μg L-1 diuron, 0.6 μg L-1 fosetyl aluminum, 0.05 μg L-1 carbaryl, and 0.7 μg L-1 glyphosate for a total concentration of 4.55 μg L-1. The total nominal concentration of pesticides mixture corresponds to the pesticide concentrations in the shellfish culture area of the Marennes-Oleron basin. Two varieties of C. gigas were selected on the foreshore, based on their characteristics in terms of resistance to summer mortality, to assess the effects of the pesticide mixture after 7 days of exposure under controlled conditions. The early effects of the mixture were assessed using enzyme biomarkers of nitrogen metabolism (GS, glutamine synthetase), detoxification metabolism (GST, glutathione S-transferase), and oxidative stress (CAT, catalase). Sublethal effects on hemocyte parameters (phagocytosis and esterase activity) and DNA damages (DNA adducts) were also measured. Changes in metabolic activities were characterized by increases in GS, GST, and CAT levels on the first day of exposure for the "resistant" oysters and after 3-7 days of exposure for the "susceptible" oysters. The formation of DNA adducts was detected after 7 days of exposure. The percentage of hemocyte esterase-positive cells was reduced in the resistant oysters, as was the hemocyte phagocytic capacity in both oyster varieties after 7 days of exposure to the pesticide mixture. This study highlights the need to consider the low doses and the mixture of pesticides to evaluate the effects of these molecules on organisms. © 2011 Wiley Periodicals, Inc. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Environmental Toxicology 28 12 689 699 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
spellingShingle |
[SDV]Life Sciences [q-bio] Geret, F. Burgeot, T. Haure, J. Gagnaire, B. Renault, T. Communal, P.Y. Samain, J.F. Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
International audience This study investigated the effects on the physiology of Pacific oyster, Crassostrea gigas, of a mixture of pesticides containing 0.8 μg L-1 alachlor, 0.6 μg L-1 metolachlor, 0.7 μg L-1 atrazine, 0.6 μg L-1 terbuthylazine, 0.5 μg L-1 diuron, 0.6 μg L-1 fosetyl aluminum, 0.05 μg L-1 carbaryl, and 0.7 μg L-1 glyphosate for a total concentration of 4.55 μg L-1. The total nominal concentration of pesticides mixture corresponds to the pesticide concentrations in the shellfish culture area of the Marennes-Oleron basin. Two varieties of C. gigas were selected on the foreshore, based on their characteristics in terms of resistance to summer mortality, to assess the effects of the pesticide mixture after 7 days of exposure under controlled conditions. The early effects of the mixture were assessed using enzyme biomarkers of nitrogen metabolism (GS, glutamine synthetase), detoxification metabolism (GST, glutathione S-transferase), and oxidative stress (CAT, catalase). Sublethal effects on hemocyte parameters (phagocytosis and esterase activity) and DNA damages (DNA adducts) were also measured. Changes in metabolic activities were characterized by increases in GS, GST, and CAT levels on the first day of exposure for the "resistant" oysters and after 3-7 days of exposure for the "susceptible" oysters. The formation of DNA adducts was detected after 7 days of exposure. The percentage of hemocyte esterase-positive cells was reduced in the resistant oysters, as was the hemocyte phagocytic capacity in both oyster varieties after 7 days of exposure to the pesticide mixture. This study highlights the need to consider the low doses and the mixture of pesticides to evaluate the effects of these molecules on organisms. © 2011 Wiley Periodicals, Inc. |
author2 |
Géographie de l'environnement (GEODE) Université Toulouse - Jean Jaurès (UT2J)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Radioécologie et d'Ecotoxicologie (DEI/SECRE/LRE) Institut de Radioprotection et de Sûreté Nucléaire (IRSN) University of Angers Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Geret, F. Burgeot, T. Haure, J. Gagnaire, B. Renault, T. Communal, P.Y. Samain, J.F. |
author_facet |
Geret, F. Burgeot, T. Haure, J. Gagnaire, B. Renault, T. Communal, P.Y. Samain, J.F. |
author_sort |
Geret, F. |
title |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_short |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_full |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_fullStr |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_sort |
effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.archives-ouvertes.fr/hal-02865179 https://doi.org/10.1002/tox.20764 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1520-4081 Environmental Toxicology https://hal.archives-ouvertes.fr/hal-02865179 Environmental Toxicology, 2013, 28 (12), pp.689-699. ⟨10.1002/tox.20764⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/tox.20764 hal-02865179 https://hal.archives-ouvertes.fr/hal-02865179 doi:10.1002/tox.20764 |
op_doi |
https://doi.org/10.1002/tox.20764 |
container_title |
Environmental Toxicology |
container_volume |
28 |
container_issue |
12 |
container_start_page |
689 |
op_container_end_page |
699 |
_version_ |
1766394073414893568 |