Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs
International audience Application of sperm cryopreservation by the fish farming industry is still limited, despite great interest in optimising genetic resource conservation; limiting risks associated with horizontal disease transmission; increasing genetic progress; and accelerating genetic select...
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ftccsdartic:oai:HAL:hal-02757877v1 2023-05-15T18:41:12+02:00 Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs Haffray, Pierrick Labbé, Catherine IMV Technologies, - Maisse, Gérard Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF) Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) IMV Technologies Saint-Malo, France 2007-06-03 https://hal.inrae.fr/hal-02757877 en eng HAL CCSD MNHN Museum National d'Histoire Naturelle hal-02757877 https://hal.inrae.fr/hal-02757877 PRODINRA: 30032 WOS: 000267276400054 Cybium 8. International Symposium on Reproductive Physiology of Fish https://hal.inrae.fr/hal-02757877 8. International Symposium on Reproductive Physiology of Fish, Jun 2007, Saint-Malo, France http://sfi.mnhn.fr/cybium/numeros/2008/322%20suppl%20ISRPF/sommaire322Suppl.html IMV fish sperm cryopreservation gene bank [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] info:eu-repo/semantics/conferenceObject Conference papers 2007 ftccsdartic 2021-08-07T22:55:12Z International audience Application of sperm cryopreservation by the fish farming industry is still limited, despite great interest in optimising genetic resource conservation; limiting risks associated with horizontal disease transmission; increasing genetic progress; and accelerating genetic selection from prime specimens, as in other livestock production. Several factors are listed. With the aim of developing coherent broodstock selection programmes, SYSAAF (Union of the French poultry and aquaculture broodstock industry), INRA and IMV-Technologies, standardised semen cryopreservation procedures between 1994 and 2000. The first step was to develop multispecies (salmonids, turbot, sea bream, sea bass, gold fish, tilapia, European catfish) extenders to collect, induce maturation and conserve (> 7 days) fresh sperm or testicular spermatozoa (StorFish (TM)); to wash ova from faeces and blood (OvaFish (TM)); to freeze sperm (Cryofish (TM)); and to improve fertilisation success (ActiFish (TM)). The second step was to evaluate the practical limits of the technology and to test several potential improvements (GnRH stimulation, anaesthetic, frequency of collection, cryopreservation devices, optimal egg/sperm volumes, etc.). The third step was to standardise sperm collection procedures, from the efferent duct or testicular spermatozoa; to ensure absolute traceability by straw printing; to fill and seal a large number of straws by means of an automated filling and sealing machine (MRS1); and to freeze sperm in liquid vapour in adapted container. The entire procedure, using optimal extenders and standardised procedures, was transferred to the industry. From 2000 to 2006, 50,080 0.5 ml straws from 1554 males, originating from 26 populations of rainbow trout, sea bream, turbot and sea bass, were frozen by breeding companies. The development of a collective gene bank is in progress, with the aim of securing private gene banks (breeding companies, research organisations and Cryobanque Nationale). Conference Object Turbot Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Malo ENVELOPE(7.500,7.500,62.689,62.689) |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
IMV fish sperm cryopreservation gene bank [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] |
spellingShingle |
IMV fish sperm cryopreservation gene bank [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] Haffray, Pierrick Labbé, Catherine IMV Technologies, - Maisse, Gérard Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs |
topic_facet |
IMV fish sperm cryopreservation gene bank [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] |
description |
International audience Application of sperm cryopreservation by the fish farming industry is still limited, despite great interest in optimising genetic resource conservation; limiting risks associated with horizontal disease transmission; increasing genetic progress; and accelerating genetic selection from prime specimens, as in other livestock production. Several factors are listed. With the aim of developing coherent broodstock selection programmes, SYSAAF (Union of the French poultry and aquaculture broodstock industry), INRA and IMV-Technologies, standardised semen cryopreservation procedures between 1994 and 2000. The first step was to develop multispecies (salmonids, turbot, sea bream, sea bass, gold fish, tilapia, European catfish) extenders to collect, induce maturation and conserve (> 7 days) fresh sperm or testicular spermatozoa (StorFish (TM)); to wash ova from faeces and blood (OvaFish (TM)); to freeze sperm (Cryofish (TM)); and to improve fertilisation success (ActiFish (TM)). The second step was to evaluate the practical limits of the technology and to test several potential improvements (GnRH stimulation, anaesthetic, frequency of collection, cryopreservation devices, optimal egg/sperm volumes, etc.). The third step was to standardise sperm collection procedures, from the efferent duct or testicular spermatozoa; to ensure absolute traceability by straw printing; to fill and seal a large number of straws by means of an automated filling and sealing machine (MRS1); and to freeze sperm in liquid vapour in adapted container. The entire procedure, using optimal extenders and standardised procedures, was transferred to the industry. From 2000 to 2006, 50,080 0.5 ml straws from 1554 males, originating from 26 populations of rainbow trout, sea bream, turbot and sea bass, were frozen by breeding companies. The development of a collective gene bank is in progress, with the aim of securing private gene banks (breeding companies, research organisations and Cryobanque Nationale). |
author2 |
Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF) Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) IMV Technologies |
format |
Conference Object |
author |
Haffray, Pierrick Labbé, Catherine IMV Technologies, - Maisse, Gérard |
author_facet |
Haffray, Pierrick Labbé, Catherine IMV Technologies, - Maisse, Gérard |
author_sort |
Haffray, Pierrick |
title |
Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs |
title_short |
Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs |
title_full |
Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs |
title_fullStr |
Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs |
title_full_unstemmed |
Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs |
title_sort |
fish sperm cryopreservation in france: from laboratory studies to application in selective breeding programs |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.inrae.fr/hal-02757877 |
op_coverage |
Saint-Malo, France |
long_lat |
ENVELOPE(7.500,7.500,62.689,62.689) |
geographic |
Malo |
geographic_facet |
Malo |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Cybium 8. International Symposium on Reproductive Physiology of Fish https://hal.inrae.fr/hal-02757877 8. International Symposium on Reproductive Physiology of Fish, Jun 2007, Saint-Malo, France http://sfi.mnhn.fr/cybium/numeros/2008/322%20suppl%20ISRPF/sommaire322Suppl.html |
op_relation |
hal-02757877 https://hal.inrae.fr/hal-02757877 PRODINRA: 30032 WOS: 000267276400054 |
_version_ |
1766230691448619008 |