Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout
International audience The decapeptide gonadotropin-releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in...
Published in: | Journal of Comparative Neurology |
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Online Access: | https://hal.inrae.fr/hal-02715916 https://hal.inrae.fr/hal-02715916/document https://hal.inrae.fr/hal-02715916/file/Bailhache_1994_JCN_1.pdf https://doi.org/10.1002/cne.903470310 |
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ftccsdartic:oai:HAL:hal-02715916v1 2023-05-15T15:29:45+02:00 Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout Bailhache, T. Arazam, A. Klungland, H. Alestrom, P. Breton, Bernard Jego, P. Biologie cellulaire et reproduction Centre National de la Recherche Scientifique (CNRS) Agricultural University of Norway Station de physiologie des poissons Institut National de la Recherche Agronomique (INRA) 1994 https://hal.inrae.fr/hal-02715916 https://hal.inrae.fr/hal-02715916/document https://hal.inrae.fr/hal-02715916/file/Bailhache_1994_JCN_1.pdf https://doi.org/10.1002/cne.903470310 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1002/cne.903470310 info:eu-repo/semantics/altIdentifier/pmid/7822493 hal-02715916 https://hal.inrae.fr/hal-02715916 https://hal.inrae.fr/hal-02715916/document https://hal.inrae.fr/hal-02715916/file/Bailhache_1994_JCN_1.pdf doi:10.1002/cne.903470310 PRODINRA: 110804 PUBMED: 7822493 WOS: A1994PE22800009 info:eu-repo/semantics/OpenAccess ISSN: 0021-9967 EISSN: 1096-9861 Journal of Comparative Neurology https://hal.inrae.fr/hal-02715916 Journal of Comparative Neurology, 1994, 347, pp.444-454. ⟨10.1002/cne.903470310⟩ salmon gonadotropin-releasing hormone in situ hybridization immunocytochemistry [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 1994 ftccsdartic https://doi.org/10.1002/cne.903470310 2023-03-26T13:44:13Z International audience The decapeptide gonadotropin-releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in data concerning the distribution of sGnRH perikarya led us to investigate this problem in two species, the Atlantic salmon and the rainbow trout, with in situ hybridization of sGnRH messenger, a highly specific molecular tool. By Northern blot analysis, the rainbow trout sGnRH messenger appears to be about 500 bases in length, which is close to those isolated from Atlantic salmon or masu salmon and characterized previously. In situ hybridization with riboprobes generated with Atlantic salmon sGnRH cDNA demonstrated that sGnRH perikarya are restricted to the ventral part of olfactory bulbs, telencephalon, and preoptic area. They are distributed on a nearly continuous line extending from the olfactory bulbs to the preoptic area in both salmonid species studied. Despite the presence of GnRH-like immunoreactivity in the preoptic magnocellular nucleus (NPOm) and in the tegmentum of the midbrain (MT), the sGnRH mRNA is not present in these two structures. Stained cells in NPOm could be target cells for GnRH and immunoreactive neurons in MT are likely to be chicken GnRH-II containing cells. Our study not only gives a precise distribution of the sGnRH system in two salmonids, Atlantic salmon and rainbow trout, but also clarifies the ambiguous data published up to now in rainbow trout Article in Journal/Newspaper Atlantic salmon Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Journal of Comparative Neurology 347 3 444 454 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
salmon gonadotropin-releasing hormone in situ hybridization immunocytochemistry [SDV]Life Sciences [q-bio] |
spellingShingle |
salmon gonadotropin-releasing hormone in situ hybridization immunocytochemistry [SDV]Life Sciences [q-bio] Bailhache, T. Arazam, A. Klungland, H. Alestrom, P. Breton, Bernard Jego, P. Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
topic_facet |
salmon gonadotropin-releasing hormone in situ hybridization immunocytochemistry [SDV]Life Sciences [q-bio] |
description |
International audience The decapeptide gonadotropin-releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in data concerning the distribution of sGnRH perikarya led us to investigate this problem in two species, the Atlantic salmon and the rainbow trout, with in situ hybridization of sGnRH messenger, a highly specific molecular tool. By Northern blot analysis, the rainbow trout sGnRH messenger appears to be about 500 bases in length, which is close to those isolated from Atlantic salmon or masu salmon and characterized previously. In situ hybridization with riboprobes generated with Atlantic salmon sGnRH cDNA demonstrated that sGnRH perikarya are restricted to the ventral part of olfactory bulbs, telencephalon, and preoptic area. They are distributed on a nearly continuous line extending from the olfactory bulbs to the preoptic area in both salmonid species studied. Despite the presence of GnRH-like immunoreactivity in the preoptic magnocellular nucleus (NPOm) and in the tegmentum of the midbrain (MT), the sGnRH mRNA is not present in these two structures. Stained cells in NPOm could be target cells for GnRH and immunoreactive neurons in MT are likely to be chicken GnRH-II containing cells. Our study not only gives a precise distribution of the sGnRH system in two salmonids, Atlantic salmon and rainbow trout, but also clarifies the ambiguous data published up to now in rainbow trout |
author2 |
Biologie cellulaire et reproduction Centre National de la Recherche Scientifique (CNRS) Agricultural University of Norway Station de physiologie des poissons Institut National de la Recherche Agronomique (INRA) |
format |
Article in Journal/Newspaper |
author |
Bailhache, T. Arazam, A. Klungland, H. Alestrom, P. Breton, Bernard Jego, P. |
author_facet |
Bailhache, T. Arazam, A. Klungland, H. Alestrom, P. Breton, Bernard Jego, P. |
author_sort |
Bailhache, T. |
title |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_short |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_full |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_fullStr |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_full_unstemmed |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_sort |
localization of salmon gonadotropin releasing hormone mrna and peptide in brain of atlantic salmon and rainbow trout |
publisher |
HAL CCSD |
publishDate |
1994 |
url |
https://hal.inrae.fr/hal-02715916 https://hal.inrae.fr/hal-02715916/document https://hal.inrae.fr/hal-02715916/file/Bailhache_1994_JCN_1.pdf https://doi.org/10.1002/cne.903470310 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
ISSN: 0021-9967 EISSN: 1096-9861 Journal of Comparative Neurology https://hal.inrae.fr/hal-02715916 Journal of Comparative Neurology, 1994, 347, pp.444-454. ⟨10.1002/cne.903470310⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/cne.903470310 info:eu-repo/semantics/altIdentifier/pmid/7822493 hal-02715916 https://hal.inrae.fr/hal-02715916 https://hal.inrae.fr/hal-02715916/document https://hal.inrae.fr/hal-02715916/file/Bailhache_1994_JCN_1.pdf doi:10.1002/cne.903470310 PRODINRA: 110804 PUBMED: 7822493 WOS: A1994PE22800009 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1002/cne.903470310 |
container_title |
Journal of Comparative Neurology |
container_volume |
347 |
container_issue |
3 |
container_start_page |
444 |
op_container_end_page |
454 |
_version_ |
1766360186402897920 |