Lipase-catalyzed synthesis of canola phytosterols oleate esters as cholesterol lowering agents

• Published in: Enzyme and Microbial Technology
• Main Authors: Villeneuve, Pierre, Turon, F., Caro, Y., Escoffier, R., Barea, Bruno, Barouh, B., Lago, R., Piombo, Georges, Pina, Michel
• Other Authors: Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Bertin Technologies
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2005
• Subjects: ESTERIFICATION; STEROLS ESTER; PAPAYER; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; Rugosa; Antarc*; Antarctica
• Online Access: https://hal.inrae.fr/hal-02680463; https://doi.org/10.1016/j.enzmictec.2005.02.002

Corresponding author. E-mail address: [e-mail] International audience The feasibility of lipase-catalyzed esterification of canola phytosterols with oleic acid was established. The influence of various reactions parameters was evaluated. Among the tested lipases, namely plant lipases from Carica papaya, Ricinus communis (castor bean lipase) or microbial lipases from Rhizomucor miehei, Candida antarctica B or Candida rugosa, the latter appeared to be the most appropriate resulting in a overall production of steryl ester of 38.5% within 24 h in non-optimized conditions whereas esterification degree with other lipases did not exceed 15% in the same conditions. Selecting C. rugosa lipase for any further experiments, it was observed that the nature of the acyl donor was of paramount importance with direct esterification with fatty acids giving better results than transesterification with methyl esters. Similarly, the influence of substrates molar ratio was also checked and a 1:3 excess of fatty acids was shown to be the best. Finally, others parameters such as optimal enzyme load (5%) or temperature (35 ◦C) were determined to allow a final production of steryl esters close to 85% after 72 h.