A novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids
International audience A cDNA encoding for a novel rainbow trout SHBG was identified and characterized. Phylogenetic analysis showed that this novel SHBG, named SHBGb, was a highly divergent paralog of the classical SHBG (SHBGa) form previously known in vertebrates including zebrafish, seabass, and...
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Online Access: | https://hal.inrae.fr/hal-02668537 https://doi.org/10.1210/en.2007-1652 |
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ftccsdartic:oai:HAL:hal-02668537v1 2023-05-15T15:32:42+02:00 A novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids Bobe, Julien Mahé, Sophie Nguyen, Thuy Thao Vi Rime, Hélène Vizziano, Denise Fostier, Alexis Guiguen, Yann Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) 2008 https://hal.inrae.fr/hal-02668537 https://doi.org/10.1210/en.2007-1652 en eng HAL CCSD Endocrine Society info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2007-1652 hal-02668537 https://hal.inrae.fr/hal-02668537 doi:10.1210/en.2007-1652 PRODINRA: 23543 WOS: 000256053100035 ISSN: 0013-7227 Endocrinology https://hal.inrae.fr/hal-02668537 Endocrinology, Endocrine Society, 2008, 149 (6), pp.2980-2989. ⟨10.1210/en.2007-1652⟩ http://press.endocrine.org/journal/endo salmonid steroid-binding protein genomics [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism info:eu-repo/semantics/article Journal articles 2008 ftccsdartic https://doi.org/10.1210/en.2007-1652 2021-08-21T22:43:45Z International audience A cDNA encoding for a novel rainbow trout SHBG was identified and characterized. Phylogenetic analysis showed that this novel SHBG, named SHBGb, was a highly divergent paralog of the classical SHBG (SHBGa) form previously known in vertebrates including zebrafish, seabass, and rainbow trout. Using all available sequences, no SHBGb-like sequence could be identified in any fish species besides Atlantic salmon. Rainbow trout SHBGa and SHBGb share only 26% sequence identity at the amino acid level and exhibit totally distinct tissue distribution, thus demonstrating a functional shift of SHBGb. Indeed, shbga mRNA was predominantly expressed in liver and spleen but could not be detected in the ovary, whereas shbgb had a predominant ovarian expression but could not be detected in liver. Despite its high divergence, rainbow trout SHBGb expressed in COS-7 cells could bind estradiol and testosterone with high affinity and specificity. Both rainbow trout shbgb mRNA and proteins were localized to the granulosa cells of vitellogenic ovarian follicles, whereas SHBGb immunoreactivity was also found in theca cells. Finally, shbgb ovarian mRNA expression exhibited a significant drop between late vitellogenesis and oocyte maturation at a time when ovarian aromatase (cyp19a) gene expression and estradiol circulating levels exhibited a dramatic decrease. Together, these observations show that SHBGb is a functional and highly divergent SHBG paralog probably arising from a salmonid-specific duplication of the shbg gene. Article in Journal/Newspaper Atlantic salmon Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Endocrinology 149 6 2980 2989 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
salmonid steroid-binding protein genomics [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism |
spellingShingle |
salmonid steroid-binding protein genomics [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Bobe, Julien Mahé, Sophie Nguyen, Thuy Thao Vi Rime, Hélène Vizziano, Denise Fostier, Alexis Guiguen, Yann A novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids |
topic_facet |
salmonid steroid-binding protein genomics [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism |
description |
International audience A cDNA encoding for a novel rainbow trout SHBG was identified and characterized. Phylogenetic analysis showed that this novel SHBG, named SHBGb, was a highly divergent paralog of the classical SHBG (SHBGa) form previously known in vertebrates including zebrafish, seabass, and rainbow trout. Using all available sequences, no SHBGb-like sequence could be identified in any fish species besides Atlantic salmon. Rainbow trout SHBGa and SHBGb share only 26% sequence identity at the amino acid level and exhibit totally distinct tissue distribution, thus demonstrating a functional shift of SHBGb. Indeed, shbga mRNA was predominantly expressed in liver and spleen but could not be detected in the ovary, whereas shbgb had a predominant ovarian expression but could not be detected in liver. Despite its high divergence, rainbow trout SHBGb expressed in COS-7 cells could bind estradiol and testosterone with high affinity and specificity. Both rainbow trout shbgb mRNA and proteins were localized to the granulosa cells of vitellogenic ovarian follicles, whereas SHBGb immunoreactivity was also found in theca cells. Finally, shbgb ovarian mRNA expression exhibited a significant drop between late vitellogenesis and oocyte maturation at a time when ovarian aromatase (cyp19a) gene expression and estradiol circulating levels exhibited a dramatic decrease. Together, these observations show that SHBGb is a functional and highly divergent SHBG paralog probably arising from a salmonid-specific duplication of the shbg gene. |
author2 |
Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) |
format |
Article in Journal/Newspaper |
author |
Bobe, Julien Mahé, Sophie Nguyen, Thuy Thao Vi Rime, Hélène Vizziano, Denise Fostier, Alexis Guiguen, Yann |
author_facet |
Bobe, Julien Mahé, Sophie Nguyen, Thuy Thao Vi Rime, Hélène Vizziano, Denise Fostier, Alexis Guiguen, Yann |
author_sort |
Bobe, Julien |
title |
A novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids |
title_short |
A novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids |
title_full |
A novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids |
title_fullStr |
A novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids |
title_full_unstemmed |
A novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids |
title_sort |
novel, functional, and highly divergent sex hormone-binding globulin that may participate in the local control of ovarian functions in salmonids |
publisher |
HAL CCSD |
publishDate |
2008 |
url |
https://hal.inrae.fr/hal-02668537 https://doi.org/10.1210/en.2007-1652 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
ISSN: 0013-7227 Endocrinology https://hal.inrae.fr/hal-02668537 Endocrinology, Endocrine Society, 2008, 149 (6), pp.2980-2989. ⟨10.1210/en.2007-1652⟩ http://press.endocrine.org/journal/endo |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2007-1652 hal-02668537 https://hal.inrae.fr/hal-02668537 doi:10.1210/en.2007-1652 PRODINRA: 23543 WOS: 000256053100035 |
op_doi |
https://doi.org/10.1210/en.2007-1652 |
container_title |
Endocrinology |
container_volume |
149 |
container_issue |
6 |
container_start_page |
2980 |
op_container_end_page |
2989 |
_version_ |
1766363197338550272 |