Microbial succession of nitrate-reducting bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps
International audience Changes in community structure and activity of the dissimilatory nitrate-reducing community were investigated across a glacier foreland in the Central Alps to gain insight into the successional pattern of this functional group and the driving environmental factors. Bulk soil a...
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Online Access: | https://hal.inrae.fr/hal-02666566 https://doi.org/10.1111/j.1462-2920.2006.01051.x |
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ftccsdartic:oai:HAL:hal-02666566v1 2023-05-15T18:01:39+02:00 Microbial succession of nitrate-reducting bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps Deiglmayr, K. Philippot, Laurent, Tscherko, D. Kandeler, Ellen University of Hohenheim Microbiologie Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB) 2006 https://hal.inrae.fr/hal-02666566 https://doi.org/10.1111/j.1462-2920.2006.01051.x en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1462-2920.2006.01051.x hal-02666566 https://hal.inrae.fr/hal-02666566 doi:10.1111/j.1462-2920.2006.01051.x PRODINRA: 30906 WOS: 000239510600011 ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.inrae.fr/hal-02666566 Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2006, 8 (9), pp.1600 - 1612. ⟨10.1111/j.1462-2920.2006.01051.x⟩ Poa alpina community structure molecular anlysis diversity ecosystem glacier soil pâturin des alpes [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2006 ftccsdartic https://doi.org/10.1111/j.1462-2920.2006.01051.x 2021-04-17T22:40:30Z International audience Changes in community structure and activity of the dissimilatory nitrate-reducing community were investigated across a glacier foreland in the Central Alps to gain insight into the successional pattern of this functional group and the driving environmental factors. Bulk soil and rhizosphere soil of Poa alpina was sampled in five replicates in August during the flowering stage and in September after the first snowfalls along a gradient from 25 to 129 years after deglaciation and at a reference site outside the glacier foreland (> 2000 years deglaciated). In a laboratory-based assay, nitrate reductase activity was determined colorimetrically after 24 h of anaerobic incubation. In selected rhizosphere soil samples, the community structure of nitrate-reducing microorganisms was analysed by restriction fragment length polymorphism (RFLP) analysis using degenerate primers for the narG gene encoding the active site of the membrane-bound nitrate reductase. Clone libraries of the early (25 years) and late (129 years) succession were constructed and representative clones sequenced. The activity of the nitrate-reducing community increased significantly with age mainly due to higher carbon and nitrate availability in the late succession. The community structure, however, only showed a small shift over the 100 years of soil formation with pH explaining a major part (19%) of the observed variance. Clone library analysis of the early and late succession pointed to a trend of declining diversity with progressing age. Presumably, the pressure of competition on the nitrate reducers was relatively low in the early successional stage due to minor densities of microorganisms compared with the late stage; hence, a higher diversity could persist in this sparse environment. These results suggest that the nitrate reductase activity is regulated by environmental factors other than those shaping the genetic structure of the nitrate-reducing community. Article in Journal/Newspaper Poa alpina Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Environmental Microbiology 8 9 1600 1612 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Poa alpina community structure molecular anlysis diversity ecosystem glacier soil pâturin des alpes [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
Poa alpina community structure molecular anlysis diversity ecosystem glacier soil pâturin des alpes [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Deiglmayr, K. Philippot, Laurent, Tscherko, D. Kandeler, Ellen Microbial succession of nitrate-reducting bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps |
topic_facet |
Poa alpina community structure molecular anlysis diversity ecosystem glacier soil pâturin des alpes [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
International audience Changes in community structure and activity of the dissimilatory nitrate-reducing community were investigated across a glacier foreland in the Central Alps to gain insight into the successional pattern of this functional group and the driving environmental factors. Bulk soil and rhizosphere soil of Poa alpina was sampled in five replicates in August during the flowering stage and in September after the first snowfalls along a gradient from 25 to 129 years after deglaciation and at a reference site outside the glacier foreland (> 2000 years deglaciated). In a laboratory-based assay, nitrate reductase activity was determined colorimetrically after 24 h of anaerobic incubation. In selected rhizosphere soil samples, the community structure of nitrate-reducing microorganisms was analysed by restriction fragment length polymorphism (RFLP) analysis using degenerate primers for the narG gene encoding the active site of the membrane-bound nitrate reductase. Clone libraries of the early (25 years) and late (129 years) succession were constructed and representative clones sequenced. The activity of the nitrate-reducing community increased significantly with age mainly due to higher carbon and nitrate availability in the late succession. The community structure, however, only showed a small shift over the 100 years of soil formation with pH explaining a major part (19%) of the observed variance. Clone library analysis of the early and late succession pointed to a trend of declining diversity with progressing age. Presumably, the pressure of competition on the nitrate reducers was relatively low in the early successional stage due to minor densities of microorganisms compared with the late stage; hence, a higher diversity could persist in this sparse environment. These results suggest that the nitrate reductase activity is regulated by environmental factors other than those shaping the genetic structure of the nitrate-reducing community. |
author2 |
University of Hohenheim Microbiologie Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB) |
format |
Article in Journal/Newspaper |
author |
Deiglmayr, K. Philippot, Laurent, Tscherko, D. Kandeler, Ellen |
author_facet |
Deiglmayr, K. Philippot, Laurent, Tscherko, D. Kandeler, Ellen |
author_sort |
Deiglmayr, K. |
title |
Microbial succession of nitrate-reducting bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps |
title_short |
Microbial succession of nitrate-reducting bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps |
title_full |
Microbial succession of nitrate-reducting bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps |
title_fullStr |
Microbial succession of nitrate-reducting bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps |
title_full_unstemmed |
Microbial succession of nitrate-reducting bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps |
title_sort |
microbial succession of nitrate-reducting bacteria in the rhizosphere of poa alpina across a glacier foreland in the central alps |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.inrae.fr/hal-02666566 https://doi.org/10.1111/j.1462-2920.2006.01051.x |
genre |
Poa alpina |
genre_facet |
Poa alpina |
op_source |
ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.inrae.fr/hal-02666566 Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2006, 8 (9), pp.1600 - 1612. ⟨10.1111/j.1462-2920.2006.01051.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1462-2920.2006.01051.x hal-02666566 https://hal.inrae.fr/hal-02666566 doi:10.1111/j.1462-2920.2006.01051.x PRODINRA: 30906 WOS: 000239510600011 |
op_doi |
https://doi.org/10.1111/j.1462-2920.2006.01051.x |
container_title |
Environmental Microbiology |
container_volume |
8 |
container_issue |
9 |
container_start_page |
1600 |
op_container_end_page |
1612 |
_version_ |
1766171153504665600 |