Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia)
International audience Samples of water from the hot springs of Uzon Caldera with temperatures from 68 to 87 C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polym...
Published in: | Applied and Environmental Microbiology |
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Online Access: | https://hal.inrae.fr/hal-02662645 https://doi.org/10.1128/AEM.00607-08 |
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ftccsdartic:oai:HAL:hal-02662645v1 2023-09-26T15:19:38+02:00 Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) Kublanov, Ilya Perevalova, Anna Slobodkina, Galina Lebedinsky, Aleksander Bidzhieva, Salima Kolganova, Tatyana Kaliberda, Elena Rumsh, Lev Haertlé, Thomas Bonch-Osmolovskaya, Elizaveta Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) Institut National de la Recherche Agronomique (INRA) Winogradsky Institute of Microbiology Moscow Russian Academy of Sciences Moscow (RAS) Bioengineering Center Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry 2009 https://hal.inrae.fr/hal-02662645 https://doi.org/10.1128/AEM.00607-08 en eng HAL CCSD American Society for Microbiology info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.00607-08 hal-02662645 https://hal.inrae.fr/hal-02662645 doi:10.1128/AEM.00607-08 PRODINRA: 31473 PUBMEDCENTRAL: PMC2612224 WOS: 000262084800037 ISSN: 0099-2240 EISSN: 1098-5336 Applied and Environmental Microbiology https://hal.inrae.fr/hal-02662645 Applied and Environmental Microbiology, 2009, 75 (1), pp.286-291. ⟨10.1128/AEM.00607-08⟩ THERMOSTABILITE THERMOPHILIC MICROORGANISM THERMOPHILIC BACTERIA HYDROLASE BIODIVERSITY HEAT RESISTANCE ENZYMATIC HYDROLYSIS ENZYMATIC ACTIVITY PHYLOGENY DICTYOGLOMUS THERMOTOGALES MICROORGANISME THERMOPHILE BACTERIE THERMOPHILE TOLERANCE A LA CHALEUR BIODIVERSITE PHYLOGENIE ACTIVIVITE ENZYMATIQUE HYDROLYSE ENZYMATIQUE CALDICELLULOSIRUPTOR [SDV.EE]Life Sciences [q-bio]/Ecology environment info:eu-repo/semantics/article Journal articles 2009 ftccsdartic https://doi.org/10.1128/AEM.00607-08 2023-08-26T22:45:24Z International audience Samples of water from the hot springs of Uzon Caldera with temperatures from 68 to 87 C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial hot springs. Article in Journal/Newspaper Kamchatka Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Applied and Environmental Microbiology 75 1 286 291 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
THERMOSTABILITE THERMOPHILIC MICROORGANISM THERMOPHILIC BACTERIA HYDROLASE BIODIVERSITY HEAT RESISTANCE ENZYMATIC HYDROLYSIS ENZYMATIC ACTIVITY PHYLOGENY DICTYOGLOMUS THERMOTOGALES MICROORGANISME THERMOPHILE BACTERIE THERMOPHILE TOLERANCE A LA CHALEUR BIODIVERSITE PHYLOGENIE ACTIVIVITE ENZYMATIQUE HYDROLYSE ENZYMATIQUE CALDICELLULOSIRUPTOR [SDV.EE]Life Sciences [q-bio]/Ecology environment |
spellingShingle |
THERMOSTABILITE THERMOPHILIC MICROORGANISM THERMOPHILIC BACTERIA HYDROLASE BIODIVERSITY HEAT RESISTANCE ENZYMATIC HYDROLYSIS ENZYMATIC ACTIVITY PHYLOGENY DICTYOGLOMUS THERMOTOGALES MICROORGANISME THERMOPHILE BACTERIE THERMOPHILE TOLERANCE A LA CHALEUR BIODIVERSITE PHYLOGENIE ACTIVIVITE ENZYMATIQUE HYDROLYSE ENZYMATIQUE CALDICELLULOSIRUPTOR [SDV.EE]Life Sciences [q-bio]/Ecology environment Kublanov, Ilya Perevalova, Anna Slobodkina, Galina Lebedinsky, Aleksander Bidzhieva, Salima Kolganova, Tatyana Kaliberda, Elena Rumsh, Lev Haertlé, Thomas Bonch-Osmolovskaya, Elizaveta Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) |
topic_facet |
THERMOSTABILITE THERMOPHILIC MICROORGANISM THERMOPHILIC BACTERIA HYDROLASE BIODIVERSITY HEAT RESISTANCE ENZYMATIC HYDROLYSIS ENZYMATIC ACTIVITY PHYLOGENY DICTYOGLOMUS THERMOTOGALES MICROORGANISME THERMOPHILE BACTERIE THERMOPHILE TOLERANCE A LA CHALEUR BIODIVERSITE PHYLOGENIE ACTIVIVITE ENZYMATIQUE HYDROLYSE ENZYMATIQUE CALDICELLULOSIRUPTOR [SDV.EE]Life Sciences [q-bio]/Ecology environment |
description |
International audience Samples of water from the hot springs of Uzon Caldera with temperatures from 68 to 87 C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial hot springs. |
author2 |
Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) Institut National de la Recherche Agronomique (INRA) Winogradsky Institute of Microbiology Moscow Russian Academy of Sciences Moscow (RAS) Bioengineering Center Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry |
format |
Article in Journal/Newspaper |
author |
Kublanov, Ilya Perevalova, Anna Slobodkina, Galina Lebedinsky, Aleksander Bidzhieva, Salima Kolganova, Tatyana Kaliberda, Elena Rumsh, Lev Haertlé, Thomas Bonch-Osmolovskaya, Elizaveta |
author_facet |
Kublanov, Ilya Perevalova, Anna Slobodkina, Galina Lebedinsky, Aleksander Bidzhieva, Salima Kolganova, Tatyana Kaliberda, Elena Rumsh, Lev Haertlé, Thomas Bonch-Osmolovskaya, Elizaveta |
author_sort |
Kublanov, Ilya |
title |
Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) |
title_short |
Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) |
title_full |
Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) |
title_fullStr |
Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) |
title_full_unstemmed |
Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia) |
title_sort |
biodiversity of thermophilic prokaryotes with hydrolytic activities in hot springs of uzon caldera, kamchatka (russia) |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.inrae.fr/hal-02662645 https://doi.org/10.1128/AEM.00607-08 |
genre |
Kamchatka |
genre_facet |
Kamchatka |
op_source |
ISSN: 0099-2240 EISSN: 1098-5336 Applied and Environmental Microbiology https://hal.inrae.fr/hal-02662645 Applied and Environmental Microbiology, 2009, 75 (1), pp.286-291. ⟨10.1128/AEM.00607-08⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.00607-08 hal-02662645 https://hal.inrae.fr/hal-02662645 doi:10.1128/AEM.00607-08 PRODINRA: 31473 PUBMEDCENTRAL: PMC2612224 WOS: 000262084800037 |
op_doi |
https://doi.org/10.1128/AEM.00607-08 |
container_title |
Applied and Environmental Microbiology |
container_volume |
75 |
container_issue |
1 |
container_start_page |
286 |
op_container_end_page |
291 |
_version_ |
1778143027818135552 |