In vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the GFP marker gene
International audience A caprine arthritis encephalitis virus (CAEV), carrying the green fluorescent protein (GFP) into the tat region was recently reported [.Mselli-Lakhal, L., Guiguen, F., Greenland, T., Mornex, J.F., Chebloune, Y., 2006. Gene transfer system derived from the caprine arthritis-enc...
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Online Access: | https://hal.inrae.fr/hal-02659358 https://doi.org/10.1016/j.jviromet.2007.01.035 |
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ftccsdartic:oai:HAL:hal-02659358v1 2023-05-15T16:29:38+02:00 In vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the GFP marker gene Mselli-Lakhal, Laila Guiguen, François Greenland, Timothy Mornex, Jean-François Chebloune, Yahia Rétrovirus et Pathologie Comparée (RPC) Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL) 2007 https://hal.inrae.fr/hal-02659358 https://doi.org/10.1016/j.jviromet.2007.01.035 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2007.01.035 hal-02659358 https://hal.inrae.fr/hal-02659358 doi:10.1016/j.jviromet.2007.01.035 PRODINRA: 143887 WOS: 000247297300002 ISSN: 0166-0934 Journal of Virological Methods https://hal.inrae.fr/hal-02659358 Journal of Virological Methods, Elsevier, 2007, 143 (1), pp.11-15. ⟨10.1016/j.jviromet.2007.01.035⟩ CAEV GFP TROPISM RELATION VIRUS-VECTEUR [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology info:eu-repo/semantics/article Journal articles 2007 ftccsdartic https://doi.org/10.1016/j.jviromet.2007.01.035 2021-11-21T00:47:37Z International audience A caprine arthritis encephalitis virus (CAEV), carrying the green fluorescent protein (GFP) into the tat region was recently reported [.Mselli-Lakhal, L., Guiguen, F., Greenland, T., Mornex, J.F., Chebloune, Y., 2006. Gene transfer system derived from the caprine arthritis-encephalitis lentivirus. J. Virol. Meth. 136, 177-184]. This construct, called pK2EGFPH replicated to titres up to 10(5) IU/ml on infection of caprine cells, and could be concentrated to 106 IU/ml by ultracentrifugation. In the present study, the pK2EGFPH construct was characterized better and used in cross-species infection studies. The pK2EGFPH virus could transduce GFP protein expression both to goat synovial membrane cells and to an immortalized goat milk epithelial cell line. The pK2EGFPH infected cells were demonstrated to express both GFP protein and CAEV viral proteins, as demonstrated by radioimmunoprecipitation and multinucleated cell formation. However GFP expression could not be maintained over passages. This vector was used to investigate cross-species infectious potential of CAEV. The bovine cell lines MDBK and GBK were found to be sensitive to infection while the human cell lines Hela, A431 and THP-1 were not. The pK2EGFPH vector should prove useful in studies of CAEV tropism both in vitro and in vivo. Article in Journal/Newspaper Greenland Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Greenland Journal of Virological Methods 143 1 11 15 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
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English |
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CAEV GFP TROPISM RELATION VIRUS-VECTEUR [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
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CAEV GFP TROPISM RELATION VIRUS-VECTEUR [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Mselli-Lakhal, Laila Guiguen, François Greenland, Timothy Mornex, Jean-François Chebloune, Yahia In vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the GFP marker gene |
topic_facet |
CAEV GFP TROPISM RELATION VIRUS-VECTEUR [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
description |
International audience A caprine arthritis encephalitis virus (CAEV), carrying the green fluorescent protein (GFP) into the tat region was recently reported [.Mselli-Lakhal, L., Guiguen, F., Greenland, T., Mornex, J.F., Chebloune, Y., 2006. Gene transfer system derived from the caprine arthritis-encephalitis lentivirus. J. Virol. Meth. 136, 177-184]. This construct, called pK2EGFPH replicated to titres up to 10(5) IU/ml on infection of caprine cells, and could be concentrated to 106 IU/ml by ultracentrifugation. In the present study, the pK2EGFPH construct was characterized better and used in cross-species infection studies. The pK2EGFPH virus could transduce GFP protein expression both to goat synovial membrane cells and to an immortalized goat milk epithelial cell line. The pK2EGFPH infected cells were demonstrated to express both GFP protein and CAEV viral proteins, as demonstrated by radioimmunoprecipitation and multinucleated cell formation. However GFP expression could not be maintained over passages. This vector was used to investigate cross-species infectious potential of CAEV. The bovine cell lines MDBK and GBK were found to be sensitive to infection while the human cell lines Hela, A431 and THP-1 were not. The pK2EGFPH vector should prove useful in studies of CAEV tropism both in vitro and in vivo. |
author2 |
Rétrovirus et Pathologie Comparée (RPC) Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL) |
format |
Article in Journal/Newspaper |
author |
Mselli-Lakhal, Laila Guiguen, François Greenland, Timothy Mornex, Jean-François Chebloune, Yahia |
author_facet |
Mselli-Lakhal, Laila Guiguen, François Greenland, Timothy Mornex, Jean-François Chebloune, Yahia |
author_sort |
Mselli-Lakhal, Laila |
title |
In vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the GFP marker gene |
title_short |
In vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the GFP marker gene |
title_full |
In vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the GFP marker gene |
title_fullStr |
In vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the GFP marker gene |
title_full_unstemmed |
In vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the GFP marker gene |
title_sort |
in vitro cross-species infections using a caprine arthritis encephalitis lentivirus carrying the gfp marker gene |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.inrae.fr/hal-02659358 https://doi.org/10.1016/j.jviromet.2007.01.035 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
ISSN: 0166-0934 Journal of Virological Methods https://hal.inrae.fr/hal-02659358 Journal of Virological Methods, Elsevier, 2007, 143 (1), pp.11-15. ⟨10.1016/j.jviromet.2007.01.035⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2007.01.035 hal-02659358 https://hal.inrae.fr/hal-02659358 doi:10.1016/j.jviromet.2007.01.035 PRODINRA: 143887 WOS: 000247297300002 |
op_doi |
https://doi.org/10.1016/j.jviromet.2007.01.035 |
container_title |
Journal of Virological Methods |
container_volume |
143 |
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1 |
container_start_page |
11 |
op_container_end_page |
15 |
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1766019337971302400 |