Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture
International audience Marine mammal Brucella strains with different genomic characteristics according to distribution of IS711 elements in their genomes were analysed for their intracellular behaviour in human THP-1 macrophage-like cells. Seven different groups of marine mammal strains were identif...
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ftccsdartic:oai:HAL:hal-02658617v1 2023-05-15T15:59:53+02:00 Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture Maquart, Marianne Zygmunt, Michel Cloeckaert, Axel Infectiologie Animale et Santé Publique (UR IASP) Institut National de la Recherche Agronomique (INRA) 2009 https://hal.inrae.fr/hal-02658617 https://doi.org/10.1016/j.micinf.2008.12.012 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2008.12.012 hal-02658617 https://hal.inrae.fr/hal-02658617 doi:10.1016/j.micinf.2008.12.012 PRODINRA: 144520 WOS: 000265402100006 ISSN: 1286-4579 Microbes and Infection https://hal.inrae.fr/hal-02658617 Microbes and Infection, Elsevier, 2009, 11 (3), pp.361-366. ⟨10.1016/j.micinf.2008.12.012⟩ HOODED SEAL MACROPHAGES SOUTHERN BLOT IS711 BRUCELLA MARINE MAMMAL INFECTION [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2009 ftccsdartic https://doi.org/10.1016/j.micinf.2008.12.012 2021-05-08T22:50:33Z International audience Marine mammal Brucella strains with different genomic characteristics according to distribution of IS711 elements in their genomes were analysed for their intracellular behaviour in human THP-1 macrophage-like cells. Seven different groups of marine mammal strains were identified including a human isolate from New Zealand presumably from marine origin. Entry and intracellular survival of strains representative of these groups in THP-1 human macrophage-like cells were analysed at several times of infection. Three patterns of infection were identified. The Brucella strain isolated from the human case from New Zealand, and two other groups of strains belonging to B. ceti or B. pinnipedialis were able to infect THP-1 macrophage cells to the same extent as the virulent strains B. suis 1330 or B. melitensis 16M. Three other groups of strains belonging to B. ceti or B. pinnipedialis were able to enter the cells as classical virulent strains but were eliminated after 48 h. The last group was composed only of strains isolated from hooded seals (Cystophora cristata) and was even unable to enter and infect THP-1 macrophage cells. Thus, several groups of marine mammal Brucella strains appear to be non-infectious for human macrophages. (C) 2009 Elsevier Masson SAS. All fights reserved. Article in Journal/Newspaper Cystophora cristata hooded seal Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) New Zealand Microbes and Infection 11 3 361 366 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
HOODED SEAL MACROPHAGES SOUTHERN BLOT IS711 BRUCELLA MARINE MAMMAL INFECTION [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
HOODED SEAL MACROPHAGES SOUTHERN BLOT IS711 BRUCELLA MARINE MAMMAL INFECTION [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Maquart, Marianne Zygmunt, Michel Cloeckaert, Axel Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture |
topic_facet |
HOODED SEAL MACROPHAGES SOUTHERN BLOT IS711 BRUCELLA MARINE MAMMAL INFECTION [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
International audience Marine mammal Brucella strains with different genomic characteristics according to distribution of IS711 elements in their genomes were analysed for their intracellular behaviour in human THP-1 macrophage-like cells. Seven different groups of marine mammal strains were identified including a human isolate from New Zealand presumably from marine origin. Entry and intracellular survival of strains representative of these groups in THP-1 human macrophage-like cells were analysed at several times of infection. Three patterns of infection were identified. The Brucella strain isolated from the human case from New Zealand, and two other groups of strains belonging to B. ceti or B. pinnipedialis were able to infect THP-1 macrophage cells to the same extent as the virulent strains B. suis 1330 or B. melitensis 16M. Three other groups of strains belonging to B. ceti or B. pinnipedialis were able to enter the cells as classical virulent strains but were eliminated after 48 h. The last group was composed only of strains isolated from hooded seals (Cystophora cristata) and was even unable to enter and infect THP-1 macrophage cells. Thus, several groups of marine mammal Brucella strains appear to be non-infectious for human macrophages. (C) 2009 Elsevier Masson SAS. All fights reserved. |
author2 |
Infectiologie Animale et Santé Publique (UR IASP) Institut National de la Recherche Agronomique (INRA) |
format |
Article in Journal/Newspaper |
author |
Maquart, Marianne Zygmunt, Michel Cloeckaert, Axel |
author_facet |
Maquart, Marianne Zygmunt, Michel Cloeckaert, Axel |
author_sort |
Maquart, Marianne |
title |
Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture |
title_short |
Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture |
title_full |
Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture |
title_fullStr |
Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture |
title_full_unstemmed |
Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture |
title_sort |
marine mammal brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.inrae.fr/hal-02658617 https://doi.org/10.1016/j.micinf.2008.12.012 |
geographic |
New Zealand |
geographic_facet |
New Zealand |
genre |
Cystophora cristata hooded seal |
genre_facet |
Cystophora cristata hooded seal |
op_source |
ISSN: 1286-4579 Microbes and Infection https://hal.inrae.fr/hal-02658617 Microbes and Infection, Elsevier, 2009, 11 (3), pp.361-366. ⟨10.1016/j.micinf.2008.12.012⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2008.12.012 hal-02658617 https://hal.inrae.fr/hal-02658617 doi:10.1016/j.micinf.2008.12.012 PRODINRA: 144520 WOS: 000265402100006 |
op_doi |
https://doi.org/10.1016/j.micinf.2008.12.012 |
container_title |
Microbes and Infection |
container_volume |
11 |
container_issue |
3 |
container_start_page |
361 |
op_container_end_page |
366 |
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1766395785751035904 |