Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture

International audience Marine mammal Brucella strains with different genomic characteristics according to distribution of IS711 elements in their genomes were analysed for their intracellular behaviour in human THP-1 macrophage-like cells. Seven different groups of marine mammal strains were identif...

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Bibliographic Details
Published in:Microbes and Infection
Main Authors: Maquart, Marianne, Zygmunt, Michel, Cloeckaert, Axel
Other Authors: Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2009
Subjects:
HOODED SEAL
MACROPHAGES
SOUTHERN BLOT
IS711
BRUCELLA
MARINE MAMMAL
INFECTION
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
New Zealand
Cystophora cristata
hooded seal
Online Access:https://hal.inrae.fr/hal-02658617
https://doi.org/10.1016/j.micinf.2008.12.012
Description
Summary:International audience Marine mammal Brucella strains with different genomic characteristics according to distribution of IS711 elements in their genomes were analysed for their intracellular behaviour in human THP-1 macrophage-like cells. Seven different groups of marine mammal strains were identified including a human isolate from New Zealand presumably from marine origin. Entry and intracellular survival of strains representative of these groups in THP-1 human macrophage-like cells were analysed at several times of infection. Three patterns of infection were identified. The Brucella strain isolated from the human case from New Zealand, and two other groups of strains belonging to B. ceti or B. pinnipedialis were able to infect THP-1 macrophage cells to the same extent as the virulent strains B. suis 1330 or B. melitensis 16M. Three other groups of strains belonging to B. ceti or B. pinnipedialis were able to enter the cells as classical virulent strains but were eliminated after 48 h. The last group was composed only of strains isolated from hooded seals (Cystophora cristata) and was even unable to enter and infect THP-1 macrophage cells. Thus, several groups of marine mammal Brucella strains appear to be non-infectious for human macrophages. (C) 2009 Elsevier Masson SAS. All fights reserved.

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