Quantitative HPLC determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm
International audience Quantification of outputs of antibacterial agents from fish farms is necessary to assess their environmental impact. In effluents, antibacterial agents are dissolved or associated with mineral or organic materials, the heaviest of which quickly settle at the exit of the fish f...
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ftccsdartic:oai:HAL:hal-02653176v1 2023-05-15T18:41:10+02:00 Quantitative HPLC determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm Pouliquen, Herve Le Bris, Hervé Thorin, Chantal Larhantec-Verdier, Michaëlle Morvan, Marie Line Chimiothérapie Aquacole et Environnement (CAE) Ecole Nationale Vétérinaire de Nantes-Institut National de la Recherche Agronomique (INRA) 2006 https://hal.inrae.fr/hal-02653176 en eng HAL CCSD Akadémiai Kiadó hal-02653176 https://hal.inrae.fr/hal-02653176 PRODINRA: 145838 WOS: ISI:000244171500011 ISSN: 1233-2356 Acta Chromatographica https://hal.inrae.fr/hal-02653176 Acta Chromatographica, Akadémiai Kiadó, 2006, pp.173-187 ANTIBACTERIAL AGENTS MARINE-SEDIMENTS ANTIBIOTICS PERFORMANCE AQUATIC ENVIRONMENT RESIDUES [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2006 ftccsdartic 2021-06-19T22:51:16Z International audience Quantification of outputs of antibacterial agents from fish farms is necessary to assess their environmental impact. In effluents, antibacterial agents are dissolved or associated with mineral or organic materials, the heaviest of which quickly settle at the exit of the fish farm. Oxolinic acid (OA) is one of the quinolones the most frequently used in turbot farms. For the first time, procedures are described for determination of dissolved, suspended, and settled forms of OA by reversed-phase high-performance liquid chromatography. Chromatography was performed on a 5 mu m C-18 cartridge, with a mixture of acetonitrile and aqueous orthophosphoric acid solution as mobile phase, and fluorescence detection. OA was easily and completely isolated by solid-liquid extraction. The method is selective, accurate, and precise; limits of quantitation were 0.005 mu g mL(-1), 0.025 mu g per filter, and 0.025 mu g g(-1), respectively for dissolved, suspended, and settled forms of OA. These methods enabled initial assessment of the amounts of dissolved, suspended, and settled forms of OA (72, 23, and 2%, respectively) of the antibacterial agent administered. Article in Journal/Newspaper Turbot Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
ANTIBACTERIAL AGENTS MARINE-SEDIMENTS ANTIBIOTICS PERFORMANCE AQUATIC ENVIRONMENT RESIDUES [SDV]Life Sciences [q-bio] |
spellingShingle |
ANTIBACTERIAL AGENTS MARINE-SEDIMENTS ANTIBIOTICS PERFORMANCE AQUATIC ENVIRONMENT RESIDUES [SDV]Life Sciences [q-bio] Pouliquen, Herve Le Bris, Hervé Thorin, Chantal Larhantec-Verdier, Michaëlle Morvan, Marie Line Quantitative HPLC determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm |
topic_facet |
ANTIBACTERIAL AGENTS MARINE-SEDIMENTS ANTIBIOTICS PERFORMANCE AQUATIC ENVIRONMENT RESIDUES [SDV]Life Sciences [q-bio] |
description |
International audience Quantification of outputs of antibacterial agents from fish farms is necessary to assess their environmental impact. In effluents, antibacterial agents are dissolved or associated with mineral or organic materials, the heaviest of which quickly settle at the exit of the fish farm. Oxolinic acid (OA) is one of the quinolones the most frequently used in turbot farms. For the first time, procedures are described for determination of dissolved, suspended, and settled forms of OA by reversed-phase high-performance liquid chromatography. Chromatography was performed on a 5 mu m C-18 cartridge, with a mixture of acetonitrile and aqueous orthophosphoric acid solution as mobile phase, and fluorescence detection. OA was easily and completely isolated by solid-liquid extraction. The method is selective, accurate, and precise; limits of quantitation were 0.005 mu g mL(-1), 0.025 mu g per filter, and 0.025 mu g g(-1), respectively for dissolved, suspended, and settled forms of OA. These methods enabled initial assessment of the amounts of dissolved, suspended, and settled forms of OA (72, 23, and 2%, respectively) of the antibacterial agent administered. |
author2 |
Chimiothérapie Aquacole et Environnement (CAE) Ecole Nationale Vétérinaire de Nantes-Institut National de la Recherche Agronomique (INRA) |
format |
Article in Journal/Newspaper |
author |
Pouliquen, Herve Le Bris, Hervé Thorin, Chantal Larhantec-Verdier, Michaëlle Morvan, Marie Line |
author_facet |
Pouliquen, Herve Le Bris, Hervé Thorin, Chantal Larhantec-Verdier, Michaëlle Morvan, Marie Line |
author_sort |
Pouliquen, Herve |
title |
Quantitative HPLC determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm |
title_short |
Quantitative HPLC determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm |
title_full |
Quantitative HPLC determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm |
title_fullStr |
Quantitative HPLC determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm |
title_full_unstemmed |
Quantitative HPLC determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm |
title_sort |
quantitative hplc determination and preliminary assessment of dissolved, suspended, and settled oxolinic acid from turbot farm |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.inrae.fr/hal-02653176 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
ISSN: 1233-2356 Acta Chromatographica https://hal.inrae.fr/hal-02653176 Acta Chromatographica, Akadémiai Kiadó, 2006, pp.173-187 |
op_relation |
hal-02653176 https://hal.inrae.fr/hal-02653176 PRODINRA: 145838 WOS: ISI:000244171500011 |
_version_ |
1766230656382140416 |