Cytochrome P450 1A1 expression in cetacean skin biopsies from the Indian Ocean

• Published in: Marine Pollution Bulletin
• Main Authors: Jauniaux, Thierry, Farnir, Frédéric, Fontaine, Michaël, Kiszka, Jérémy, Sarlet, Michael, Coignoul, Freddy
• Other Authors: Université de Liège, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Paris-Sud - Paris 11 (UP11), Ecologie Systématique et Evolution (ESE), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11), Université de La Rochelle (ULR)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2011
• Subjects: Cytochrome P450; Biopsy; Skin; Immunohistochemistry; Mayotte; PORPOISES PHOCOENA-PHOCOENA; BOTTLE-NOSED-DOLPHIN; TURSIOPS-TRUNCATUS; INTEGUMENT; MARINE MAMMALS; CONTAMINANTS; POLLUTANTS; INDUCTION; EXPOSURE; [SDV]Life Sciences [q-bio]; Indian; Megaptera novaeangliae; Phocoena phocoena
• Online Access: https://hal.inrae.fr/hal-02652593; https://doi.org/10.1016/j.marpolbul.2011.04.005

International audience The study describes cytochrome P450 1A1 (CYPA1) expression in the skin of different cetacean species (Megaptera novaeangliae, n = 15; Stenella attenuata, n = 7 and Stenella longirostris, n = 24) from the Mozambique Channel island of Mayotte. Immunohistochemical examination was performed with a monoclonal antibody against scup cytochrome CYPA1. The sex was determined using a molecular approach consisting in the genotyping sex-specific genes. CYPA1 was detected at the junction between epidermis and blubber on dolphins only, mostly in the endothelial cells. Similar observation was obtained in the dermis of one M. novaeangliae. Immunohistochemical slides were scored to evaluate the expression of the CYPA1 and a higher expression was observed in S. longirostris, suggesting a higher exposure to pollutants for this species. The difference of expression between sexes was not significant. (C) 2011 Elsevier Ltd. All rights reserved.