Molecular epidemiology of Flavobacterium psychrophilum from Swiss fish farms
International audience Bacterial cold water disease (BCWD) and rainbow trout fry syndrome (RTFS) caused by Flavobacterium psychrophilum are 2 of the major diseases causing high fish mortality in salmonid fish farms. The molecular epidemiology of F. psychrophilum is still largely unknown. Multilocus...
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ftccsdartic:oai:HAL:hal-02651608v1 2023-05-15T18:09:58+02:00 Molecular epidemiology of Flavobacterium psychrophilum from Swiss fish farms Strepparava, Nicole Nicolas, Pierre, Wahli, Thomas Segner, Helmut Petrini, Orlando Cantonal Institute of Microbiology Unité Mathématique Informatique et Génome (MIG) Institut National de la Recherche Agronomique (INRA) University of Bern EU EMIDA ERA-NET project PATHOFISH ANR 2010-EMID-006-01 Swiss Federal Veterinary Office (FVO) 2013 https://hal.inrae.fr/hal-02651608 https://doi.org/10.3354/dao02609 en eng HAL CCSD Inter Research info:eu-repo/semantics/altIdentifier/doi/10.3354/dao02609 hal-02651608 https://hal.inrae.fr/hal-02651608 doi:10.3354/dao02609 PRODINRA: 213833 WOS: 000323911300004 ISSN: 0177-5103 Diseases of Aquatic Organisms https://hal.inrae.fr/hal-02651608 Diseases of Aquatic Organisms, Inter Research, 2013, 105 (3), pp.203 - 210. ⟨10.3354/dao02609⟩ F. psychrophilum BCWD RTFS Myxobacteria Pathology Clonal complex Sequence complex Allele type BACTERIAL COLDWATER DISEASE AYU PLECOGLOSSUS-ALTIVELIS CYTOPHAGA-PSYCHROPHILA SEQUENCE DIVERSITY EBURST MLST Flavobacteriosis SALMON SALMO-SALAR POPULATION-STRUCTURE [MATH]Mathematics [math] [INFO]Computer Science [cs] [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.3354/dao02609 2021-09-11T23:40:03Z International audience Bacterial cold water disease (BCWD) and rainbow trout fry syndrome (RTFS) caused by Flavobacterium psychrophilum are 2 of the major diseases causing high fish mortality in salmonid fish farms. The molecular epidemiology of F. psychrophilum is still largely unknown. Multilocus sequence typing (MLST) has been previously used for this pathogen and underscored a correlation between clonal complexes and host fish species. Here we used MLST to study the relationships among 112 F. psychrophilum isolates from rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta fario and S. t. lacustris in Swiss fish farms between 1993 and 2012. The isolates belonged to 27 different sequence types (STs). Most of the Swiss outbreaks were associated with strains belonging to clonal complexes CC-ST2 and CC-ST90, found in both rainbow trout and brown trout and represented by several STs. Eight ST singletons could not be connected to any known clonal complex. Already reported from other parts of Europe and North America, CC-ST2 was the most frequent clonal complex observed, and it caused the majority of outbreaks in Switzerland, with CC-ST90 being the second most important type. In the tightly interconnected Swiss fish farms, no association between clonal complex and host fish was detected, but a temporal evolution of the frequency of some STs was observed. The occurrence of sporadic STs suggests high F. psychrophilum diversity and may reflect the presence of different sequence types in the environment. Article in Journal/Newspaper Salmo salar Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Diseases of Aquatic Organisms 105 3 203 210 |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
F. psychrophilum BCWD RTFS Myxobacteria Pathology Clonal complex Sequence complex Allele type BACTERIAL COLDWATER DISEASE AYU PLECOGLOSSUS-ALTIVELIS CYTOPHAGA-PSYCHROPHILA SEQUENCE DIVERSITY EBURST MLST Flavobacteriosis SALMON SALMO-SALAR POPULATION-STRUCTURE [MATH]Mathematics [math] [INFO]Computer Science [cs] [SDV]Life Sciences [q-bio] |
spellingShingle |
F. psychrophilum BCWD RTFS Myxobacteria Pathology Clonal complex Sequence complex Allele type BACTERIAL COLDWATER DISEASE AYU PLECOGLOSSUS-ALTIVELIS CYTOPHAGA-PSYCHROPHILA SEQUENCE DIVERSITY EBURST MLST Flavobacteriosis SALMON SALMO-SALAR POPULATION-STRUCTURE [MATH]Mathematics [math] [INFO]Computer Science [cs] [SDV]Life Sciences [q-bio] Strepparava, Nicole Nicolas, Pierre, Wahli, Thomas Segner, Helmut Petrini, Orlando Molecular epidemiology of Flavobacterium psychrophilum from Swiss fish farms |
topic_facet |
F. psychrophilum BCWD RTFS Myxobacteria Pathology Clonal complex Sequence complex Allele type BACTERIAL COLDWATER DISEASE AYU PLECOGLOSSUS-ALTIVELIS CYTOPHAGA-PSYCHROPHILA SEQUENCE DIVERSITY EBURST MLST Flavobacteriosis SALMON SALMO-SALAR POPULATION-STRUCTURE [MATH]Mathematics [math] [INFO]Computer Science [cs] [SDV]Life Sciences [q-bio] |
description |
International audience Bacterial cold water disease (BCWD) and rainbow trout fry syndrome (RTFS) caused by Flavobacterium psychrophilum are 2 of the major diseases causing high fish mortality in salmonid fish farms. The molecular epidemiology of F. psychrophilum is still largely unknown. Multilocus sequence typing (MLST) has been previously used for this pathogen and underscored a correlation between clonal complexes and host fish species. Here we used MLST to study the relationships among 112 F. psychrophilum isolates from rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta fario and S. t. lacustris in Swiss fish farms between 1993 and 2012. The isolates belonged to 27 different sequence types (STs). Most of the Swiss outbreaks were associated with strains belonging to clonal complexes CC-ST2 and CC-ST90, found in both rainbow trout and brown trout and represented by several STs. Eight ST singletons could not be connected to any known clonal complex. Already reported from other parts of Europe and North America, CC-ST2 was the most frequent clonal complex observed, and it caused the majority of outbreaks in Switzerland, with CC-ST90 being the second most important type. In the tightly interconnected Swiss fish farms, no association between clonal complex and host fish was detected, but a temporal evolution of the frequency of some STs was observed. The occurrence of sporadic STs suggests high F. psychrophilum diversity and may reflect the presence of different sequence types in the environment. |
author2 |
Cantonal Institute of Microbiology Unité Mathématique Informatique et Génome (MIG) Institut National de la Recherche Agronomique (INRA) University of Bern EU EMIDA ERA-NET project PATHOFISH ANR 2010-EMID-006-01 Swiss Federal Veterinary Office (FVO) |
format |
Article in Journal/Newspaper |
author |
Strepparava, Nicole Nicolas, Pierre, Wahli, Thomas Segner, Helmut Petrini, Orlando |
author_facet |
Strepparava, Nicole Nicolas, Pierre, Wahli, Thomas Segner, Helmut Petrini, Orlando |
author_sort |
Strepparava, Nicole |
title |
Molecular epidemiology of Flavobacterium psychrophilum from Swiss fish farms |
title_short |
Molecular epidemiology of Flavobacterium psychrophilum from Swiss fish farms |
title_full |
Molecular epidemiology of Flavobacterium psychrophilum from Swiss fish farms |
title_fullStr |
Molecular epidemiology of Flavobacterium psychrophilum from Swiss fish farms |
title_full_unstemmed |
Molecular epidemiology of Flavobacterium psychrophilum from Swiss fish farms |
title_sort |
molecular epidemiology of flavobacterium psychrophilum from swiss fish farms |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.inrae.fr/hal-02651608 https://doi.org/10.3354/dao02609 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
ISSN: 0177-5103 Diseases of Aquatic Organisms https://hal.inrae.fr/hal-02651608 Diseases of Aquatic Organisms, Inter Research, 2013, 105 (3), pp.203 - 210. ⟨10.3354/dao02609⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3354/dao02609 hal-02651608 https://hal.inrae.fr/hal-02651608 doi:10.3354/dao02609 PRODINRA: 213833 WOS: 000323911300004 |
op_doi |
https://doi.org/10.3354/dao02609 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
105 |
container_issue |
3 |
container_start_page |
203 |
op_container_end_page |
210 |
_version_ |
1766182674648530944 |