In vitro intestinal absorption of amino acid mixtures extracted from codfish ( Gadus morhua L. ) salting wastewater

• Published in: International Journal of Food Science & Technology
• Main Authors: Ferraro, Vincenza, Ferreira Jorge, Ruben, Cruz, Isabel B., Antunes, Filipa, Sarmento, Bruno, Castro, Paula M. L., Pintado, Manuela E.
• Other Authors: Universidade Católica Portuguesa, Universidade do Porto = University of Porto, Instituto Superior de Ciencias da Saude Norte, Partenaires INRAE, EU FP-6 Marie Curie Actions, National Founds from FCT - Fundacao para a Ciencia e Tecnologia PEst-OE/EQB/LA0016/2011
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2014
• Subjects: amino acids; Caco-2 cell; codfish; small intestine; absorption; [SDV.IDA]Life Sciences [q-bio]/Food engineering; Gadus morhua
• Online Access: https://hal.inrae.fr/hal-02630973; https://doi.org/10.1111/ijfs.12269

International audience This study reports on the intestinal permeability of salt-containing mixtures of amino acids extracted from codfish salting wastewater. Permeability was evaluated in vitro using the Caco-2 cell line model; cell integrity during exposure to mixtures of amino acids was estimated by measuring the transepithelial electrical resistance (TEER). The effect of salt (NaCl) on the permeability and on the intestinal cell's integrity was also examined. Permeation rate (i.e. transport) was 95% for all amino acids except for creatine, for which it was 6%. Values for apparent permeability coefficients, P-app>10(-5)cms(-1), for mixture with isotonic concentration of NaCl suggest that amino acids are very likely to be absorbed in humans. Mixture with a hypertonic level of NaCl exerts a cytotoxic effect in intestinal cells resulting in a loss of epithelium integrity. Results show that isotonic mixture of amino acids extracted from codfish salting wastewater could be used in food, feed, cosmetic and pharmaceutical formulations. These applications could contribute to the fish industry sustainability.