Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae
WOS:000447511100005 International audience Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are charac...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , , , , , |
Other Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2018
|
Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-02626098 https://hal.archives-ouvertes.fr/hal-02626098/document https://hal.archives-ouvertes.fr/hal-02626098/file/Foulon_etal_SR_2018.pdf https://doi.org/10.1038/s41598-018-33720-4 |
id |
ftccsdartic:oai:HAL:hal-02626098v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
ACL adhesion pacific oyster in-situ foot histochemistry l-dopa ostrea-edulis pediveliger star asterias-rubens tube feet [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL adhesion pacific oyster in-situ foot histochemistry l-dopa ostrea-edulis pediveliger star asterias-rubens tube feet [SDE.BE]Environmental Sciences/Biodiversity and Ecology Foulon, Valentin Artigaud, Sébastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guérard, Fabienne Boudry, Pierre Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
topic_facet |
ACL adhesion pacific oyster in-situ foot histochemistry l-dopa ostrea-edulis pediveliger star asterias-rubens tube feet [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
WOS:000447511100005 International audience Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Interactions Cellules Organismes Environnement (ICORE) CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN) Normandie Université (NU) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de Brest (UBO) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010) |
format |
Article in Journal/Newspaper |
author |
Foulon, Valentin Artigaud, Sébastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guérard, Fabienne Boudry, Pierre |
author_facet |
Foulon, Valentin Artigaud, Sébastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guérard, Fabienne Boudry, Pierre |
author_sort |
Foulon, Valentin |
title |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_short |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_full |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_fullStr |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_full_unstemmed |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_sort |
proteinaceous secretion of bioadhesive produced during crawling and settlement of crassostrea gigas larvae |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.archives-ouvertes.fr/hal-02626098 https://hal.archives-ouvertes.fr/hal-02626098/document https://hal.archives-ouvertes.fr/hal-02626098/file/Foulon_etal_SR_2018.pdf https://doi.org/10.1038/s41598-018-33720-4 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports https://hal.archives-ouvertes.fr/hal-02626098 Scientific Reports, Nature Publishing Group, 2018, 8, pp.15298. ⟨10.1038/s41598-018-33720-4⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-33720-4 hal-02626098 https://hal.archives-ouvertes.fr/hal-02626098 https://hal.archives-ouvertes.fr/hal-02626098/document https://hal.archives-ouvertes.fr/hal-02626098/file/Foulon_etal_SR_2018.pdf doi:10.1038/s41598-018-33720-4 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1038/s41598-018-33720-4 |
container_title |
Scientific Reports |
container_volume |
8 |
container_issue |
1 |
_version_ |
1766393842764873728 |
spelling |
ftccsdartic:oai:HAL:hal-02626098v1 2023-05-15T15:58:07+02:00 Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae Foulon, Valentin Artigaud, Sébastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guérard, Fabienne Boudry, Pierre Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Interactions Cellules Organismes Environnement (ICORE) CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN) Normandie Université (NU) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de Brest (UBO) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010) 2018 https://hal.archives-ouvertes.fr/hal-02626098 https://hal.archives-ouvertes.fr/hal-02626098/document https://hal.archives-ouvertes.fr/hal-02626098/file/Foulon_etal_SR_2018.pdf https://doi.org/10.1038/s41598-018-33720-4 en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-33720-4 hal-02626098 https://hal.archives-ouvertes.fr/hal-02626098 https://hal.archives-ouvertes.fr/hal-02626098/document https://hal.archives-ouvertes.fr/hal-02626098/file/Foulon_etal_SR_2018.pdf doi:10.1038/s41598-018-33720-4 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports https://hal.archives-ouvertes.fr/hal-02626098 Scientific Reports, Nature Publishing Group, 2018, 8, pp.15298. ⟨10.1038/s41598-018-33720-4⟩ ACL adhesion pacific oyster in-situ foot histochemistry l-dopa ostrea-edulis pediveliger star asterias-rubens tube feet [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1038/s41598-018-33720-4 2021-12-19T01:17:22Z WOS:000447511100005 International audience Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Scientific Reports 8 1 |