Siberian sturgeon, Acipenser baeri, spermatozoa : effects of dilution, pH, osmotic pressure, sodium and potassium ions on mobility
International audience The influence of the sperm dilution rate, pH, osmotic pressure and monovalent ion (K(+) Na(+) CI-) concentration on sperm mobility was studied on the Siberian sturgeon, Acipenser baeri. Average values of pH, osmotic pressure, K(+) and Na(+) concentration in the seminal plasma...
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1989
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ftccsdartic:oai:HAL:hal-02578091v1 2023-05-15T18:19:30+02:00 Siberian sturgeon, Acipenser baeri, spermatozoa : effects of dilution, pH, osmotic pressure, sodium and potassium ions on mobility Esturgeon de Sibérie, Acipenser Baeri, spermatozoïdes: effets de la dilution, du pH, de la pression osmotique, des ions sodium et potassium sur leur mobilité Gallis, J.L. Fedrigo, E. Jatteau, Philippe Bonpunt, E. BILLARD, R. Centre National de la Recherche Scientifique (CNRS) Université de Bordeaux (UB) Aquaculture et pêche (UR APBX) Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF) Bordeaux, France 1989-10-03 https://hal.inrae.fr/hal-02578091 en eng HAL CCSD Cemagref Editions hal-02578091 https://hal.inrae.fr/hal-02578091 IRSTEA: PUB00005570 Acipenser : actes du premier colloque international sur l'esturgeon, Bordeaux https://hal.inrae.fr/hal-02578091 Acipenser : actes du premier colloque international sur l'esturgeon, Bordeaux, Oct 1989, Bordeaux, France. pp.143-151 [SDE]Environmental Sciences info:eu-repo/semantics/conferenceObject Conference papers 1989 ftccsdartic 2021-02-27T23:55:41Z International audience The influence of the sperm dilution rate, pH, osmotic pressure and monovalent ion (K(+) Na(+) CI-) concentration on sperm mobility was studied on the Siberian sturgeon, Acipenser baeri. Average values of pH, osmotic pressure, K(+) and Na(+) concentration in the seminal plasma were respectively pH 8.1 + or - 0.8, 38 + ou - 3 mOsm/Kg, 2.5 + or - 0.3 and 28 + 7 mM. Titration curve showed a strong buffer capacity of seminal plasma due mainly to presence of phosphate. The best dilution rate in deionized water for observation of motility were about 1/100. Motility occurred in saline solutions of pH 8.2 and osmotic pressure lower than 50 mOsm/Kg. Total inhibition occurred in media of acidic pH and high osmotic pressures (100 mOsm/Kg and more but in this later case, reinitiation of motility was possible after further dilution in deionized water). Potassium at 0.1 mM was strongly, but reversibly inhibiting sperm mobility. Sodium and chloride ions in the range of seminal plasma conditions (20mM) had no effect on the intensity of sperm motility. It was concluded that in physiological conditions, K(+) concentration but not osmolality seemed to be the principal cause of sperm immotility. L'influence du taux de dilution, du pH, de la pression osmotique et de la concentrations des ions monovalents ( K(+) Na(+) CI(-)) sur la motilité des spermatozoïdes est étudiée chez l'esturgeon sibérien, Acipenser baeri. Les valeurs moyennes respectives du pH, de la pression osmotique, des concentrations de K(+) et Na(+) dans le liquide séminal sont de pH 8.1 + ou - 0.8 , 38 + ou - 3 mOsm/Kg, 2.5 + ou - 0.3 et 28 + ou - 7 mM. Le liquide séminal est en outre caractérisé par une courbe de titration correspondant à une forte capacité tampon dû à la présence de phosphate. La meilleure dilution dans l'eau déionisée afin d'observer la motilité est d'environ 1/1000. Les spermatozoïdes sont motiles pour des solutions salines à pH physiologique (8.2) et pour des pressions osmotiques inférieures à 50 MOsm/Kg. ... Conference Object Siberian sturgeon Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Gallis, J.L. Fedrigo, E. Jatteau, Philippe Bonpunt, E. BILLARD, R. Siberian sturgeon, Acipenser baeri, spermatozoa : effects of dilution, pH, osmotic pressure, sodium and potassium ions on mobility |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience The influence of the sperm dilution rate, pH, osmotic pressure and monovalent ion (K(+) Na(+) CI-) concentration on sperm mobility was studied on the Siberian sturgeon, Acipenser baeri. Average values of pH, osmotic pressure, K(+) and Na(+) concentration in the seminal plasma were respectively pH 8.1 + or - 0.8, 38 + ou - 3 mOsm/Kg, 2.5 + or - 0.3 and 28 + 7 mM. Titration curve showed a strong buffer capacity of seminal plasma due mainly to presence of phosphate. The best dilution rate in deionized water for observation of motility were about 1/100. Motility occurred in saline solutions of pH 8.2 and osmotic pressure lower than 50 mOsm/Kg. Total inhibition occurred in media of acidic pH and high osmotic pressures (100 mOsm/Kg and more but in this later case, reinitiation of motility was possible after further dilution in deionized water). Potassium at 0.1 mM was strongly, but reversibly inhibiting sperm mobility. Sodium and chloride ions in the range of seminal plasma conditions (20mM) had no effect on the intensity of sperm motility. It was concluded that in physiological conditions, K(+) concentration but not osmolality seemed to be the principal cause of sperm immotility. L'influence du taux de dilution, du pH, de la pression osmotique et de la concentrations des ions monovalents ( K(+) Na(+) CI(-)) sur la motilité des spermatozoïdes est étudiée chez l'esturgeon sibérien, Acipenser baeri. Les valeurs moyennes respectives du pH, de la pression osmotique, des concentrations de K(+) et Na(+) dans le liquide séminal sont de pH 8.1 + ou - 0.8 , 38 + ou - 3 mOsm/Kg, 2.5 + ou - 0.3 et 28 + ou - 7 mM. Le liquide séminal est en outre caractérisé par une courbe de titration correspondant à une forte capacité tampon dû à la présence de phosphate. La meilleure dilution dans l'eau déionisée afin d'observer la motilité est d'environ 1/1000. Les spermatozoïdes sont motiles pour des solutions salines à pH physiologique (8.2) et pour des pressions osmotiques inférieures à 50 MOsm/Kg. ... |
author2 |
Centre National de la Recherche Scientifique (CNRS) Université de Bordeaux (UB) Aquaculture et pêche (UR APBX) Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF) |
format |
Conference Object |
author |
Gallis, J.L. Fedrigo, E. Jatteau, Philippe Bonpunt, E. BILLARD, R. |
author_facet |
Gallis, J.L. Fedrigo, E. Jatteau, Philippe Bonpunt, E. BILLARD, R. |
author_sort |
Gallis, J.L. |
title |
Siberian sturgeon, Acipenser baeri, spermatozoa : effects of dilution, pH, osmotic pressure, sodium and potassium ions on mobility |
title_short |
Siberian sturgeon, Acipenser baeri, spermatozoa : effects of dilution, pH, osmotic pressure, sodium and potassium ions on mobility |
title_full |
Siberian sturgeon, Acipenser baeri, spermatozoa : effects of dilution, pH, osmotic pressure, sodium and potassium ions on mobility |
title_fullStr |
Siberian sturgeon, Acipenser baeri, spermatozoa : effects of dilution, pH, osmotic pressure, sodium and potassium ions on mobility |
title_full_unstemmed |
Siberian sturgeon, Acipenser baeri, spermatozoa : effects of dilution, pH, osmotic pressure, sodium and potassium ions on mobility |
title_sort |
siberian sturgeon, acipenser baeri, spermatozoa : effects of dilution, ph, osmotic pressure, sodium and potassium ions on mobility |
publisher |
HAL CCSD |
publishDate |
1989 |
url |
https://hal.inrae.fr/hal-02578091 |
op_coverage |
Bordeaux, France |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_source |
Acipenser : actes du premier colloque international sur l'esturgeon, Bordeaux https://hal.inrae.fr/hal-02578091 Acipenser : actes du premier colloque international sur l'esturgeon, Bordeaux, Oct 1989, Bordeaux, France. pp.143-151 |
op_relation |
hal-02578091 https://hal.inrae.fr/hal-02578091 IRSTEA: PUB00005570 |
_version_ |
1766196598232055808 |