ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas)
WOS:000408034700015 International audience Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Cra...
Published in: | Aquaculture |
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2017
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Online Access: | https://hal.archives-ouvertes.fr/hal-02571123 https://hal.archives-ouvertes.fr/hal-02571123/document https://hal.archives-ouvertes.fr/hal-02571123/file/Boulais_etal_Aquaculture_2016.pdf https://doi.org/10.1016/j.aquaculture.2017.05.035 |
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ftccsdartic:oai:HAL:hal-02571123v1 2023-05-15T15:58:16+02:00 ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas) Boulais, Myrina Soudant, Philippe Le Goïc, Nelly Quéré, Claudie Boudry, Pierre Suquet, Marc Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2017 https://hal.archives-ouvertes.fr/hal-02571123 https://hal.archives-ouvertes.fr/hal-02571123/document https://hal.archives-ouvertes.fr/hal-02571123/file/Boulais_etal_Aquaculture_2016.pdf https://doi.org/10.1016/j.aquaculture.2017.05.035 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2017.05.035 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02571123 https://hal.archives-ouvertes.fr/hal-02571123 https://hal.archives-ouvertes.fr/hal-02571123/document https://hal.archives-ouvertes.fr/hal-02571123/file/Boulais_etal_Aquaculture_2016.pdf doi:10.1016/j.aquaculture.2017.05.035 info:eu-repo/semantics/OpenAccess ISSN: 0044-8486 Aquaculture https://hal.archives-ouvertes.fr/hal-02571123 Aquaculture, Elsevier, 2017, 479, pp.114-119. ⟨10.1016/j.aquaculture.2017.05.035⟩ ATP content spermatozoa ACL ROS production Viability Motility quality [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology info:eu-repo/semantics/article Journal articles 2017 ftccsdartic https://doi.org/10.1016/j.aquaculture.2017.05.035 2021-12-19T01:20:39Z WOS:000408034700015 International audience Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability of fertilizing ability demonstrated using simple and multiple regressions. Our results showed that ATP content depends on both MMP and viability of spermatozoa. Using multiple regressions, 61% of the variability of the trochophore-larval yield was explained by a model combining the ATP content and the percentage of dying spermatozoa (P\textless0.001). Our results reveal that capacity of spermatozoa to maintain a high level of ATP via OXPHOS partly explains the inter-individual variability of fertilization success in the Pacific oyster. Sperm ATP content and viability assays will provide valuable tools for assessing sperm quality of this species in aquaculture production, cryopreservation, and bioassays. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Aquaculture 479 114 119 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
ATP content spermatozoa ACL ROS production Viability Motility quality [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology |
spellingShingle |
ATP content spermatozoa ACL ROS production Viability Motility quality [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology Boulais, Myrina Soudant, Philippe Le Goïc, Nelly Quéré, Claudie Boudry, Pierre Suquet, Marc ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas) |
topic_facet |
ATP content spermatozoa ACL ROS production Viability Motility quality [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology |
description |
WOS:000408034700015 International audience Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability of fertilizing ability demonstrated using simple and multiple regressions. Our results showed that ATP content depends on both MMP and viability of spermatozoa. Using multiple regressions, 61% of the variability of the trochophore-larval yield was explained by a model combining the ATP content and the percentage of dying spermatozoa (P\textless0.001). Our results reveal that capacity of spermatozoa to maintain a high level of ATP via OXPHOS partly explains the inter-individual variability of fertilization success in the Pacific oyster. Sperm ATP content and viability assays will provide valuable tools for assessing sperm quality of this species in aquaculture production, cryopreservation, and bioassays. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Soudant, Philippe Le Goïc, Nelly Quéré, Claudie Boudry, Pierre Suquet, Marc |
author_facet |
Boulais, Myrina Soudant, Philippe Le Goïc, Nelly Quéré, Claudie Boudry, Pierre Suquet, Marc |
author_sort |
Boulais, Myrina |
title |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas) |
title_short |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas) |
title_full |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas) |
title_fullStr |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas) |
title_full_unstemmed |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas) |
title_sort |
atp content and viability of spermatozoa drive variability of fertilization success in the pacific oyster (crassostrea gigas) |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.archives-ouvertes.fr/hal-02571123 https://hal.archives-ouvertes.fr/hal-02571123/document https://hal.archives-ouvertes.fr/hal-02571123/file/Boulais_etal_Aquaculture_2016.pdf https://doi.org/10.1016/j.aquaculture.2017.05.035 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0044-8486 Aquaculture https://hal.archives-ouvertes.fr/hal-02571123 Aquaculture, Elsevier, 2017, 479, pp.114-119. ⟨10.1016/j.aquaculture.2017.05.035⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2017.05.035 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02571123 https://hal.archives-ouvertes.fr/hal-02571123 https://hal.archives-ouvertes.fr/hal-02571123/document https://hal.archives-ouvertes.fr/hal-02571123/file/Boulais_etal_Aquaculture_2016.pdf doi:10.1016/j.aquaculture.2017.05.035 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2017.05.035 |
container_title |
Aquaculture |
container_volume |
479 |
container_start_page |
114 |
op_container_end_page |
119 |
_version_ |
1766393993525985280 |