Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome
International audience Pacific Oyster Mortality Syndrome (POMS) affects Crassostrea gigas oysters worldwide and causes important economic losses. Disease dynamic was recently deciphered and revealed a multiple and progressive infection caused by the Ostreid herpesvirus OsHV-1 µVar, triggering an imm...
Published in: | Frontiers in Microbiology |
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Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2020
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Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-02551728 https://hal.archives-ouvertes.fr/hal-02551728/document https://hal.archives-ouvertes.fr/hal-02551728/file/Clerissi-2020-FrontMicrob-Microbiota.pdf https://doi.org/10.3389/fmicb.2020.00311 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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language |
English |
topic |
ACL metabarcoding bacteria fitness holobiont microbiome [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL metabarcoding bacteria fitness holobiont microbiome [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Clerissi, Camille De Lorgeril, Julien Petton, Bruno Lucasson, Aude Escoubas, Jean-Michel Guéguen, Yannick Degrémont, Lionel, Mitta, Guillaume Toulza, Eve Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome |
topic_facet |
ACL metabarcoding bacteria fitness holobiont microbiome [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Pacific Oyster Mortality Syndrome (POMS) affects Crassostrea gigas oysters worldwide and causes important economic losses. Disease dynamic was recently deciphered and revealed a multiple and progressive infection caused by the Ostreid herpesvirus OsHV-1 µVar, triggering an immunosuppression followed by microbiota destabilization and bacteraemia by opportunistic bacterial pathogens. However, it remains unknown if microbiota might participate to protect oysters against POMS, and if microbiota characteristics might be predictive of oyster mortalities. To tackle this issue, we transferred full-sib progenies of resistant and susceptible oyster families from hatchery to the field during a period in favor of POMS. After 5 days of transplantation, oysters from each family were either sampled for individual microbiota analyses using 16S rRNA gene-metabarcoding or transferred into facilities to record their survival using controlled condition. As expected, all oysters from susceptible families died, and all oysters from the resistant family survived. Quantification of OsHV-1 and bacteria showed that 5 days of transplantation were long enough to contaminate oysters by POMS, but not for entering the pathogenesis process. Thus, it was possible to compare microbiota characteristics between resistant and susceptible oysters families at the early steps of infection. Strikingly, we found that microbiota evenness and abundances of Cyanobacteria (Subsection III, family I), Mycoplasmataceae, Rhodobacteraceae, and Rhodospirillaceae were significantly different between resistant and susceptible oyster families. We concluded that these microbiota characteristics might predict oyster mortalities. |
author2 |
Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) Université Paris sciences et lettres (PSL) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Génétique et Pathologie des Mollusques Marins, 17390 La Tremblade, France. (LGPMM) Santé, Génétique et Microbiologie des Mollusques (IFREMER SG2M) Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-14-CE19-0023,DECIPHER,Déchiffrage des maladies multifactorielles: cas des mortalités de l'huître(2014) ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011) ANR-10-LABX-0004,CeMEB,Mediterranean Center for Environment and Biodiversity(2010) European Project: 678589,H2020,H2020-SFS-2015-2,VIVALDI(2016) |
format |
Article in Journal/Newspaper |
author |
Clerissi, Camille De Lorgeril, Julien Petton, Bruno Lucasson, Aude Escoubas, Jean-Michel Guéguen, Yannick Degrémont, Lionel, Mitta, Guillaume Toulza, Eve |
author_facet |
Clerissi, Camille De Lorgeril, Julien Petton, Bruno Lucasson, Aude Escoubas, Jean-Michel Guéguen, Yannick Degrémont, Lionel, Mitta, Guillaume Toulza, Eve |
author_sort |
Clerissi, Camille |
title |
Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome |
title_short |
Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome |
title_full |
Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome |
title_fullStr |
Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome |
title_full_unstemmed |
Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome |
title_sort |
microbiota composition and evenness predict survival rate of oysters confronted to pacific oyster mortality syndrome |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.archives-ouvertes.fr/hal-02551728 https://hal.archives-ouvertes.fr/hal-02551728/document https://hal.archives-ouvertes.fr/hal-02551728/file/Clerissi-2020-FrontMicrob-Microbiota.pdf https://doi.org/10.3389/fmicb.2020.00311 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal.archives-ouvertes.fr/hal-02551728 Frontiers in Microbiology, Frontiers Media, 2020, 11, pp.311. ⟨10.3389/fmicb.2020.00311⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.00311 info:eu-repo/grantAgreement//678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI hal-02551728 https://hal.archives-ouvertes.fr/hal-02551728 https://hal.archives-ouvertes.fr/hal-02551728/document https://hal.archives-ouvertes.fr/hal-02551728/file/Clerissi-2020-FrontMicrob-Microbiota.pdf doi:10.3389/fmicb.2020.00311 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3389/fmicb.2020.00311 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
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1766394641843748864 |
spelling |
ftccsdartic:oai:HAL:hal-02551728v1 2023-05-15T15:58:52+02:00 Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome Clerissi, Camille De Lorgeril, Julien Petton, Bruno Lucasson, Aude Escoubas, Jean-Michel Guéguen, Yannick Degrémont, Lionel, Mitta, Guillaume Toulza, Eve Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) Université Paris sciences et lettres (PSL) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Génétique et Pathologie des Mollusques Marins, 17390 La Tremblade, France. (LGPMM) Santé, Génétique et Microbiologie des Mollusques (IFREMER SG2M) Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-14-CE19-0023,DECIPHER,Déchiffrage des maladies multifactorielles: cas des mortalités de l'huître(2014) ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011) ANR-10-LABX-0004,CeMEB,Mediterranean Center for Environment and Biodiversity(2010) European Project: 678589,H2020,H2020-SFS-2015-2,VIVALDI(2016) 2020-02-27 https://hal.archives-ouvertes.fr/hal-02551728 https://hal.archives-ouvertes.fr/hal-02551728/document https://hal.archives-ouvertes.fr/hal-02551728/file/Clerissi-2020-FrontMicrob-Microbiota.pdf https://doi.org/10.3389/fmicb.2020.00311 en eng HAL CCSD Frontiers Media info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.00311 info:eu-repo/grantAgreement//678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI hal-02551728 https://hal.archives-ouvertes.fr/hal-02551728 https://hal.archives-ouvertes.fr/hal-02551728/document https://hal.archives-ouvertes.fr/hal-02551728/file/Clerissi-2020-FrontMicrob-Microbiota.pdf doi:10.3389/fmicb.2020.00311 info:eu-repo/semantics/OpenAccess ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal.archives-ouvertes.fr/hal-02551728 Frontiers in Microbiology, Frontiers Media, 2020, 11, pp.311. ⟨10.3389/fmicb.2020.00311⟩ ACL metabarcoding bacteria fitness holobiont microbiome [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2020 ftccsdartic https://doi.org/10.3389/fmicb.2020.00311 2021-12-19T01:22:44Z International audience Pacific Oyster Mortality Syndrome (POMS) affects Crassostrea gigas oysters worldwide and causes important economic losses. Disease dynamic was recently deciphered and revealed a multiple and progressive infection caused by the Ostreid herpesvirus OsHV-1 µVar, triggering an immunosuppression followed by microbiota destabilization and bacteraemia by opportunistic bacterial pathogens. However, it remains unknown if microbiota might participate to protect oysters against POMS, and if microbiota characteristics might be predictive of oyster mortalities. To tackle this issue, we transferred full-sib progenies of resistant and susceptible oyster families from hatchery to the field during a period in favor of POMS. After 5 days of transplantation, oysters from each family were either sampled for individual microbiota analyses using 16S rRNA gene-metabarcoding or transferred into facilities to record their survival using controlled condition. As expected, all oysters from susceptible families died, and all oysters from the resistant family survived. Quantification of OsHV-1 and bacteria showed that 5 days of transplantation were long enough to contaminate oysters by POMS, but not for entering the pathogenesis process. Thus, it was possible to compare microbiota characteristics between resistant and susceptible oysters families at the early steps of infection. Strikingly, we found that microbiota evenness and abundances of Cyanobacteria (Subsection III, family I), Mycoplasmataceae, Rhodobacteraceae, and Rhodospirillaceae were significantly different between resistant and susceptible oyster families. We concluded that these microbiota characteristics might predict oyster mortalities. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Frontiers in Microbiology 11 |