Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection
International audience Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 mu Var) and to determine which metabolic pathways might be affected during infection. Animals wer...
Published in: | Journal of Proteomics |
---|---|
Main Authors: | , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2014
|
Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-02548704 https://doi.org/10.1016/j.jprot.2014.06.030 |
id |
ftccsdartic:oai:HAL:hal-02548704v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:hal-02548704v1 2023-05-15T15:57:52+02:00 Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quéré, Claudie Madec, Stéphanie Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) ANR-12-AGRO-0001,GIGASSAT,Adaptation des écosystèmes ostréicoles au changement global(2012) 2014 https://hal.archives-ouvertes.fr/hal-02548704 https://doi.org/10.1016/j.jprot.2014.06.030 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2014.06.030 hal-02548704 https://hal.archives-ouvertes.fr/hal-02548704 doi:10.1016/j.jprot.2014.06.030 ISSN: 1874-3919 EISSN: 1876-7737 Journal of Proteomics https://hal.archives-ouvertes.fr/hal-02548704 Journal of Proteomics, Elsevier, 2014, 109, pp.176--187. ⟨10.1016/j.jprot.2014.06.030⟩ Warburg ACL Crassostrea gigas Metabolism Marine bivalves Herpesvirus [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2014 ftccsdartic https://doi.org/10.1016/j.jprot.2014.06.030 2021-12-19T01:22:59Z International audience Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 mu Var) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters exhibited an increased glycolysis and VDAC accumulation, which reflect a “Warburg effect” as initially reported in cancer cells and more recently in shrimp infected with virus. The results presented here should be useful for identifying potential biomarkers of disease resistance and developing antiviral measures. (C) 2014 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Journal of Proteomics 109 176 187 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Warburg ACL Crassostrea gigas Metabolism Marine bivalves Herpesvirus [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Warburg ACL Crassostrea gigas Metabolism Marine bivalves Herpesvirus [SDE.BE]Environmental Sciences/Biodiversity and Ecology Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quéré, Claudie Madec, Stéphanie Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
topic_facet |
Warburg ACL Crassostrea gigas Metabolism Marine bivalves Herpesvirus [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 mu Var) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters exhibited an increased glycolysis and VDAC accumulation, which reflect a “Warburg effect” as initially reported in cancer cells and more recently in shrimp infected with virus. The results presented here should be useful for identifying potential biomarkers of disease resistance and developing antiviral measures. (C) 2014 Elsevier B.V. All rights reserved. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) ANR-12-AGRO-0001,GIGASSAT,Adaptation des écosystèmes ostréicoles au changement global(2012) |
format |
Article in Journal/Newspaper |
author |
Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quéré, Claudie Madec, Stéphanie |
author_facet |
Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quéré, Claudie Madec, Stéphanie |
author_sort |
Corporeau, Charlotte |
title |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_short |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_full |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_fullStr |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_full_unstemmed |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_sort |
proteomic signatures of the oyster metabolic response to herpesvirus oshv-1 μvar infection |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.archives-ouvertes.fr/hal-02548704 https://doi.org/10.1016/j.jprot.2014.06.030 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1874-3919 EISSN: 1876-7737 Journal of Proteomics https://hal.archives-ouvertes.fr/hal-02548704 Journal of Proteomics, Elsevier, 2014, 109, pp.176--187. ⟨10.1016/j.jprot.2014.06.030⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2014.06.030 hal-02548704 https://hal.archives-ouvertes.fr/hal-02548704 doi:10.1016/j.jprot.2014.06.030 |
op_doi |
https://doi.org/10.1016/j.jprot.2014.06.030 |
container_title |
Journal of Proteomics |
container_volume |
109 |
container_start_page |
176 |
op_container_end_page |
187 |
_version_ |
1766393569762869248 |