Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers
International audience In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of...
Published in: | Letters in Applied Microbiology |
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Online Access: | https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522223 https://doi.org/10.1111/lam.12143 |
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ftccsdartic:oai:HAL:hal-02522223v1 2023-05-15T13:44:26+02:00 Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers Geiger, M. Guitton, Y. Vansteelandt, Marieke Kerzaon, I. Blanchet, E. Pont, T., Frisvad, J.c. Hess, P. Pouchus, Y.F. Grovel, O. Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) 2013 https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522223 https://doi.org/10.1111/lam.12143 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/lam.12143 hal-02522223 https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522223 doi:10.1111/lam.12143 ISSN: 0266-8254 EISSN: 1472-765X Letters in Applied Microbiology https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522223 Letters in Applied Microbiology, Wiley, 2013, 57 (5), pp.385-392. ⟨10.1111/lam.12143⟩ http://onlinelibrary.wiley.com/doi/10.1111/lam.12143/abstract Shellfish Cytotoxicity Marine fungi Penicillium dereplication mass spectrometry [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.1111/lam.12143 https://doi.org/10.1111/lam.12143/abstract 2021-05-01T22:45:07Z International audience In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with other constituent identical to YES medium. When shellfish-derived strains of fungi were grown on MES medium, extracts were found to be more cytotoxic than on the YES medium for some of the strains. HPLC-UV/DAD-MS/MS dereplication of extracts from Penicillium marinum and P. restrictum strains grown on MES medium showed the enhancement of the production of some cytotoxic compounds. The mycotoxin patulin was detected in some P. antarcticum extracts, and its presence seemed to be related to their cytotoxicity. Thus, the enhancement of the toxicity of extracts obtained from shellfish-derived Penicillium strains grown on a host-derived medium, and the production of metabolites such as patulin suggests that a survey of mycotoxins in edible shellfish should be considered. Significance and Impact of the Study Penicillium strains isolated from bivalve molluscs produce extracts exhibiting a higher cytotoxicity than extracts from Penicillium strains isolated from the surrounding marine environment. The use of a mussel-based medium for cultures of some shellfish-derived strains enhances the cytotoxicity of extracts when compared with classical media. The production of cytotoxic compounds and of the mycotoxin patulin on such a host-derived medium ... Article in Journal/Newspaper Antarc* Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Letters in Applied Microbiology 57 5 385 392 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Shellfish Cytotoxicity Marine fungi Penicillium dereplication mass spectrometry [SDV]Life Sciences [q-bio] |
spellingShingle |
Shellfish Cytotoxicity Marine fungi Penicillium dereplication mass spectrometry [SDV]Life Sciences [q-bio] Geiger, M. Guitton, Y. Vansteelandt, Marieke Kerzaon, I. Blanchet, E. Pont, T., Frisvad, J.c. Hess, P. Pouchus, Y.F. Grovel, O. Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
topic_facet |
Shellfish Cytotoxicity Marine fungi Penicillium dereplication mass spectrometry [SDV]Life Sciences [q-bio] |
description |
International audience In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with other constituent identical to YES medium. When shellfish-derived strains of fungi were grown on MES medium, extracts were found to be more cytotoxic than on the YES medium for some of the strains. HPLC-UV/DAD-MS/MS dereplication of extracts from Penicillium marinum and P. restrictum strains grown on MES medium showed the enhancement of the production of some cytotoxic compounds. The mycotoxin patulin was detected in some P. antarcticum extracts, and its presence seemed to be related to their cytotoxicity. Thus, the enhancement of the toxicity of extracts obtained from shellfish-derived Penicillium strains grown on a host-derived medium, and the production of metabolites such as patulin suggests that a survey of mycotoxins in edible shellfish should be considered. Significance and Impact of the Study Penicillium strains isolated from bivalve molluscs produce extracts exhibiting a higher cytotoxicity than extracts from Penicillium strains isolated from the surrounding marine environment. The use of a mussel-based medium for cultures of some shellfish-derived strains enhances the cytotoxicity of extracts when compared with classical media. The production of cytotoxic compounds and of the mycotoxin patulin on such a host-derived medium ... |
author2 |
Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) |
format |
Article in Journal/Newspaper |
author |
Geiger, M. Guitton, Y. Vansteelandt, Marieke Kerzaon, I. Blanchet, E. Pont, T., Frisvad, J.c. Hess, P. Pouchus, Y.F. Grovel, O. |
author_facet |
Geiger, M. Guitton, Y. Vansteelandt, Marieke Kerzaon, I. Blanchet, E. Pont, T., Frisvad, J.c. Hess, P. Pouchus, Y.F. Grovel, O. |
author_sort |
Geiger, M. |
title |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_short |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_full |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_fullStr |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_full_unstemmed |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_sort |
cytotoxicity and mycotoxin production of shellfish-derived penicillium spp., a risk for shellfish consumers |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522223 https://doi.org/10.1111/lam.12143 |
genre |
Antarc* |
genre_facet |
Antarc* |
op_source |
ISSN: 0266-8254 EISSN: 1472-765X Letters in Applied Microbiology https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522223 Letters in Applied Microbiology, Wiley, 2013, 57 (5), pp.385-392. ⟨10.1111/lam.12143⟩ http://onlinelibrary.wiley.com/doi/10.1111/lam.12143/abstract |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/lam.12143 hal-02522223 https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522223 doi:10.1111/lam.12143 |
op_doi |
https://doi.org/10.1111/lam.12143 https://doi.org/10.1111/lam.12143/abstract |
container_title |
Letters in Applied Microbiology |
container_volume |
57 |
container_issue |
5 |
container_start_page |
385 |
op_container_end_page |
392 |
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1766201660055486464 |