Biological effects of polycyclic aromatic hydrocarbons (PAH) and their first metabolic products in in vivo exposed Atlantic cod ( Gadus morhua )
International audience The monitoring of the presence of polycyclic aromatic hydrocarbons (PAH) in the aquatic environment is a worldwide activity since some of these compounds are well-established carcinogens and mutagens. Contaminants in this class are in fact regarded as priority hazardous substa...
Published in: | Journal of Toxicology and Environmental Health, Part A |
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Language: | English |
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ftccsdartic:oai:HAL:hal-02471029v1 2023-05-15T15:27:22+02:00 Biological effects of polycyclic aromatic hydrocarbons (PAH) and their first metabolic products in in vivo exposed Atlantic cod ( Gadus morhua ) Pampanin, Daniela Le Goff, Jérémie Skogland, Karianne Marcucci, Cristian Øysæd, Kjell Birger Lorentzen, Marianne Jørgensen, Kåre Sydnes, Magne International Research Institute of Stavanger (IRIS) Aliments Bioprocédés Toxicologie Environnements (ABTE) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN) Normandie Université (NU) ADn'tox University of Stavanger 2016-08-02 https://hal-normandie-univ.archives-ouvertes.fr/hal-02471029 https://doi.org/10.1080/15287394.2016.1171993 en eng HAL CCSD Taylor & Francis info:eu-repo/semantics/altIdentifier/doi/10.1080/15287394.2016.1171993 info:eu-repo/semantics/altIdentifier/pmid/27484143 hal-02471029 https://hal-normandie-univ.archives-ouvertes.fr/hal-02471029 doi:10.1080/15287394.2016.1171993 PUBMED: 27484143 ISSN: 1528-7394 EISSN: 1087-2620 Journal of Toxicology and Environmental Health, Part A: Current Issues https://hal-normandie-univ.archives-ouvertes.fr/hal-02471029 Journal of Toxicology and Environmental Health, Part A: Current Issues, Taylor & Francis, 2016, 79 (13-15), pp.633-646. ⟨10.1080/15287394.2016.1171993⟩ [SDE]Environmental Sciences [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2016 ftccsdartic https://doi.org/10.1080/15287394.2016.1171993 2021-12-25T23:43:07Z International audience The monitoring of the presence of polycyclic aromatic hydrocarbons (PAH) in the aquatic environment is a worldwide activity since some of these compounds are well-established carcinogens and mutagens. Contaminants in this class are in fact regarded as priority hazardous substances for environmental pollution (Water Framework Directive 2000/60/EC). In this study, Atlantic cod (Gadus morhua) was selected to assess in vivo effects of two PAH and their first metabolic products, namely, the corresponding trans-dihydrodiols, using biological markers. Fish were exposed for 1 wk to a single PAH (naphthalene or chrysene) and its synthetic metabolites ((1R,2R)-1,2-dihydronaphthalene-1,2-diol and (1R,2R)-1,2-dihydrochrysene-1,2-diol) by intraperitoneal injection in a continuous seawater flow system. After exposure, PAH metabolism including PAH metabolites in bile and ethoxyresorufin O-deethylase (EROD) activity, oxidative stress glutathione S-transferases (GST) and catalase (CAT) activities, and genotoxicity such as DNA adducts were evaluated, as well as general health conditions including condition index (CI), hepatosomatic index (HSI), and gonadosomatic index (GSI). PAH metabolite values were low and not significantly different when measured with the fixed-wavelength fluorescence screening method, while the gas chromatography-mass spectroscopy (GC-MS) method showed an apparent dose response in fish exposed to naphthalene. DNA adduct levels ≥0.16 × 10(-8) relative adduct level (RAL) were detected. It should be noted that 0.16 × 10(-8) RAL is considered the maximal acceptable background level for this species. The other biomarkers activities of catalase, GST, and EROD did not display a particular compound- or dose-related response. The GSI values were significantly lower in some chrysene- and in both naphthalene- and naphthalene diol-exposed groups compared to control. Article in Journal/Newspaper atlantic cod Gadus morhua Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Journal of Toxicology and Environmental Health, Part A 79 13-15 633 646 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDE]Environmental Sciences [SDV]Life Sciences [q-bio] |
spellingShingle |
[SDE]Environmental Sciences [SDV]Life Sciences [q-bio] Pampanin, Daniela Le Goff, Jérémie Skogland, Karianne Marcucci, Cristian Øysæd, Kjell Birger Lorentzen, Marianne Jørgensen, Kåre Sydnes, Magne Biological effects of polycyclic aromatic hydrocarbons (PAH) and their first metabolic products in in vivo exposed Atlantic cod ( Gadus morhua ) |
topic_facet |
[SDE]Environmental Sciences [SDV]Life Sciences [q-bio] |
description |
International audience The monitoring of the presence of polycyclic aromatic hydrocarbons (PAH) in the aquatic environment is a worldwide activity since some of these compounds are well-established carcinogens and mutagens. Contaminants in this class are in fact regarded as priority hazardous substances for environmental pollution (Water Framework Directive 2000/60/EC). In this study, Atlantic cod (Gadus morhua) was selected to assess in vivo effects of two PAH and their first metabolic products, namely, the corresponding trans-dihydrodiols, using biological markers. Fish were exposed for 1 wk to a single PAH (naphthalene or chrysene) and its synthetic metabolites ((1R,2R)-1,2-dihydronaphthalene-1,2-diol and (1R,2R)-1,2-dihydrochrysene-1,2-diol) by intraperitoneal injection in a continuous seawater flow system. After exposure, PAH metabolism including PAH metabolites in bile and ethoxyresorufin O-deethylase (EROD) activity, oxidative stress glutathione S-transferases (GST) and catalase (CAT) activities, and genotoxicity such as DNA adducts were evaluated, as well as general health conditions including condition index (CI), hepatosomatic index (HSI), and gonadosomatic index (GSI). PAH metabolite values were low and not significantly different when measured with the fixed-wavelength fluorescence screening method, while the gas chromatography-mass spectroscopy (GC-MS) method showed an apparent dose response in fish exposed to naphthalene. DNA adduct levels ≥0.16 × 10(-8) relative adduct level (RAL) were detected. It should be noted that 0.16 × 10(-8) RAL is considered the maximal acceptable background level for this species. The other biomarkers activities of catalase, GST, and EROD did not display a particular compound- or dose-related response. The GSI values were significantly lower in some chrysene- and in both naphthalene- and naphthalene diol-exposed groups compared to control. |
author2 |
International Research Institute of Stavanger (IRIS) Aliments Bioprocédés Toxicologie Environnements (ABTE) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN) Normandie Université (NU) ADn'tox University of Stavanger |
format |
Article in Journal/Newspaper |
author |
Pampanin, Daniela Le Goff, Jérémie Skogland, Karianne Marcucci, Cristian Øysæd, Kjell Birger Lorentzen, Marianne Jørgensen, Kåre Sydnes, Magne |
author_facet |
Pampanin, Daniela Le Goff, Jérémie Skogland, Karianne Marcucci, Cristian Øysæd, Kjell Birger Lorentzen, Marianne Jørgensen, Kåre Sydnes, Magne |
author_sort |
Pampanin, Daniela |
title |
Biological effects of polycyclic aromatic hydrocarbons (PAH) and their first metabolic products in in vivo exposed Atlantic cod ( Gadus morhua ) |
title_short |
Biological effects of polycyclic aromatic hydrocarbons (PAH) and their first metabolic products in in vivo exposed Atlantic cod ( Gadus morhua ) |
title_full |
Biological effects of polycyclic aromatic hydrocarbons (PAH) and their first metabolic products in in vivo exposed Atlantic cod ( Gadus morhua ) |
title_fullStr |
Biological effects of polycyclic aromatic hydrocarbons (PAH) and their first metabolic products in in vivo exposed Atlantic cod ( Gadus morhua ) |
title_full_unstemmed |
Biological effects of polycyclic aromatic hydrocarbons (PAH) and their first metabolic products in in vivo exposed Atlantic cod ( Gadus morhua ) |
title_sort |
biological effects of polycyclic aromatic hydrocarbons (pah) and their first metabolic products in in vivo exposed atlantic cod ( gadus morhua ) |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-02471029 https://doi.org/10.1080/15287394.2016.1171993 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
ISSN: 1528-7394 EISSN: 1087-2620 Journal of Toxicology and Environmental Health, Part A: Current Issues https://hal-normandie-univ.archives-ouvertes.fr/hal-02471029 Journal of Toxicology and Environmental Health, Part A: Current Issues, Taylor & Francis, 2016, 79 (13-15), pp.633-646. ⟨10.1080/15287394.2016.1171993⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1080/15287394.2016.1171993 info:eu-repo/semantics/altIdentifier/pmid/27484143 hal-02471029 https://hal-normandie-univ.archives-ouvertes.fr/hal-02471029 doi:10.1080/15287394.2016.1171993 PUBMED: 27484143 |
op_doi |
https://doi.org/10.1080/15287394.2016.1171993 |
container_title |
Journal of Toxicology and Environmental Health, Part A |
container_volume |
79 |
container_issue |
13-15 |
container_start_page |
633 |
op_container_end_page |
646 |
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1766357801929539584 |