Differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in European eel (Anguilla anguilla)
International audience In captivity, oogenesis and ovarian follicle maturation in European eel can be induced experimentally using hormonal therapy. The follicle's ability to respond effectively to the induction of maturation and ovulation, resulting in viable eggs, depends on the oocyte stage...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Online Access: | https://hal.archives-ouvertes.fr/hal-02407095 https://doi.org/10.1016/j.cbpa.2018.03.010 |
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ftccsdartic:oai:HAL:hal-02407095v1 2023-05-15T13:28:08+02:00 Differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in European eel (Anguilla anguilla) da Silva, Filipa F.G. Tveiten, Helge Maugars, Gersende Lafont, Anne-Gaëlle Dufour, Sylvie Støttrup, Josianne Kjørsvik, Elin Tomkiewicz, Jonna DTU Aqua, National Institute of Aquatic Resources Technical University of Denmark Lyngby (DTU) Norwegian Institute of Food,Fisheries and Aquaculture Research (NOFIMA) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Biologie des organismes marins et écosystèmes (BOME) Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN) Norwegian University of Science and Technology Trondheim (NTNU) Norwegian University of Science and Technology (NTNU) 2018-07 https://hal.archives-ouvertes.fr/hal-02407095 https://doi.org/10.1016/j.cbpa.2018.03.010 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2018.03.010 hal-02407095 https://hal.archives-ouvertes.fr/hal-02407095 doi:10.1016/j.cbpa.2018.03.010 ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal.archives-ouvertes.fr/hal-02407095 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2018, 221, pp.44-54. ⟨10.1016/j.cbpa.2018.03.010⟩ Estrogen receptors Gonadotropin receptors Lipid droplet Follicular maturation Egg quality [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1016/j.cbpa.2018.03.010 2021-12-05T01:41:07Z International audience In captivity, oogenesis and ovarian follicle maturation in European eel can be induced experimentally using hormonal therapy. The follicle's ability to respond effectively to the induction of maturation and ovulation, resulting in viable eggs, depends on the oocyte stage at the time of induction. We hypothesized that variation in the expression of key hormone receptors in the ovary and size of oocyte lipid droplets are associated with changes in oocyte stage. Thus, we induced ovarian follicle maturation using a priming dose of fish pituitary extract followed by the administration of a 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) injection. Females were then strip-spawned, the eggs were fertilized in vitro, incubated and larval survival was recorded at 3 days post hatch (dph). The expression of gonadotropin receptors (fshr, lhcgr1 and lhcgr2) and estrogen receptors (esr1, esr2a, esr2b, gpera and gperb) was quantified and the size of oocyte lipid droplets measured. Larval survival at 3 dph was used to differentiate high- and low-quality egg batches. Results showed significantly higher abundance of lhcgr1 and esr2a at priming for high-quality egg batches whereas fshr and gperb transcripts were significantly higher at DHP injection for low-quality egg batches. Therefore, high levels of lhcgr1 and esr2a may be important for attaining follicular maturational competence, while high fshr and gperb mRNA levels may indicate inadequate maturational competence. Furthermore, lipid droplet size at DHP and in ovulated eggs was significantly smaller in high-quality egg batches than in low-quality, which indicates that droplet size may be a useful marker of follicular maturational stage. Article in Journal/Newspaper Anguilla anguilla Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 221 44 54 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Estrogen receptors Gonadotropin receptors Lipid droplet Follicular maturation Egg quality [SDV]Life Sciences [q-bio] |
spellingShingle |
Estrogen receptors Gonadotropin receptors Lipid droplet Follicular maturation Egg quality [SDV]Life Sciences [q-bio] da Silva, Filipa F.G. Tveiten, Helge Maugars, Gersende Lafont, Anne-Gaëlle Dufour, Sylvie Støttrup, Josianne Kjørsvik, Elin Tomkiewicz, Jonna Differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in European eel (Anguilla anguilla) |
topic_facet |
Estrogen receptors Gonadotropin receptors Lipid droplet Follicular maturation Egg quality [SDV]Life Sciences [q-bio] |
description |
International audience In captivity, oogenesis and ovarian follicle maturation in European eel can be induced experimentally using hormonal therapy. The follicle's ability to respond effectively to the induction of maturation and ovulation, resulting in viable eggs, depends on the oocyte stage at the time of induction. We hypothesized that variation in the expression of key hormone receptors in the ovary and size of oocyte lipid droplets are associated with changes in oocyte stage. Thus, we induced ovarian follicle maturation using a priming dose of fish pituitary extract followed by the administration of a 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) injection. Females were then strip-spawned, the eggs were fertilized in vitro, incubated and larval survival was recorded at 3 days post hatch (dph). The expression of gonadotropin receptors (fshr, lhcgr1 and lhcgr2) and estrogen receptors (esr1, esr2a, esr2b, gpera and gperb) was quantified and the size of oocyte lipid droplets measured. Larval survival at 3 dph was used to differentiate high- and low-quality egg batches. Results showed significantly higher abundance of lhcgr1 and esr2a at priming for high-quality egg batches whereas fshr and gperb transcripts were significantly higher at DHP injection for low-quality egg batches. Therefore, high levels of lhcgr1 and esr2a may be important for attaining follicular maturational competence, while high fshr and gperb mRNA levels may indicate inadequate maturational competence. Furthermore, lipid droplet size at DHP and in ovulated eggs was significantly smaller in high-quality egg batches than in low-quality, which indicates that droplet size may be a useful marker of follicular maturational stage. |
author2 |
DTU Aqua, National Institute of Aquatic Resources Technical University of Denmark Lyngby (DTU) Norwegian Institute of Food,Fisheries and Aquaculture Research (NOFIMA) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Biologie des organismes marins et écosystèmes (BOME) Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN) Norwegian University of Science and Technology Trondheim (NTNU) Norwegian University of Science and Technology (NTNU) |
format |
Article in Journal/Newspaper |
author |
da Silva, Filipa F.G. Tveiten, Helge Maugars, Gersende Lafont, Anne-Gaëlle Dufour, Sylvie Støttrup, Josianne Kjørsvik, Elin Tomkiewicz, Jonna |
author_facet |
da Silva, Filipa F.G. Tveiten, Helge Maugars, Gersende Lafont, Anne-Gaëlle Dufour, Sylvie Støttrup, Josianne Kjørsvik, Elin Tomkiewicz, Jonna |
author_sort |
da Silva, Filipa F.G. |
title |
Differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in European eel (Anguilla anguilla) |
title_short |
Differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in European eel (Anguilla anguilla) |
title_full |
Differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in European eel (Anguilla anguilla) |
title_fullStr |
Differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in European eel (Anguilla anguilla) |
title_full_unstemmed |
Differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in European eel (Anguilla anguilla) |
title_sort |
differential expression of gonadotropin and estrogen receptors and oocyte cytology during follicular maturation associated with egg viability in european eel (anguilla anguilla) |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.archives-ouvertes.fr/hal-02407095 https://doi.org/10.1016/j.cbpa.2018.03.010 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal.archives-ouvertes.fr/hal-02407095 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2018, 221, pp.44-54. ⟨10.1016/j.cbpa.2018.03.010⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2018.03.010 hal-02407095 https://hal.archives-ouvertes.fr/hal-02407095 doi:10.1016/j.cbpa.2018.03.010 |
op_doi |
https://doi.org/10.1016/j.cbpa.2018.03.010 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
221 |
container_start_page |
44 |
op_container_end_page |
54 |
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1766402409082388480 |