Distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the G11 Nyegga pockmark, Norwegian Sea
Pockmarks are seabed geological structures sustaining methane seepage in cold seeps. Based on RNA-derived sequences the active fraction of the archaeal community was analysed in sediments associated with the G11 pockmark, in the Nyegga region of the Norwegian Sea. The anaerobic methanotrophic Archae...
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Online Access: | https://hal.archives-ouvertes.fr/hal-02372889 https://doi.org/10.1007/s10482-011-9620-z |
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ftccsdartic:oai:HAL:hal-02372889v1 2023-05-15T17:47:02+02:00 Distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the G11 Nyegga pockmark, Norwegian Sea Lazar, Cassandre Sara Dinasquet, Julie L'Haridon, Stéphane Pignet, Patricia Toffin, Laurent Department of Marine Sciences University of North Carolina Chapel Hill (UNC) University of North Carolina System (UNC)-University of North Carolina System (UNC) Laboratoire de microbiologie des environnements extrêmophiles (LM2E) Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2011 https://hal.archives-ouvertes.fr/hal-02372889 https://doi.org/10.1007/s10482-011-9620-z en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10482-011-9620-z hal-02372889 https://hal.archives-ouvertes.fr/hal-02372889 doi:10.1007/s10482-011-9620-z ISSN: 0003-6072 EISSN: 1572-9699 Antonie van Leeuwenhoek https://hal.archives-ouvertes.fr/hal-02372889 Antonie van Leeuwenhoek, Springer Verlag, 2011, 100 (4), pp.639-653. ⟨10.1007/s10482-011-9620-z⟩ Pockmark Cold seeps Archaea ANME SRB [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2011 ftccsdartic https://doi.org/10.1007/s10482-011-9620-z 2021-10-17T00:18:51Z Pockmarks are seabed geological structures sustaining methane seepage in cold seeps. Based on RNA-derived sequences the active fraction of the archaeal community was analysed in sediments associated with the G11 pockmark, in the Nyegga region of the Norwegian Sea. The anaerobic methanotrophic Archaea (ANME) and sulfate-reducing bacteria (SRB) communities were studied as well. The vertical distribution of the archaeal community assessed by PCR-DGGE highlighted the presence of ANME-2 in surface sediments, and ANME-1 in deeper sediments. Enrichments of methanogens showed the presence of hydrogenotrophic methanogens of the Methanogenium genus in surface sediment layers as well. The active fraction of the archaeal community was uniquely composed of ANME-2 in the shallow sulfate-rich sediments. Functional methyl coenzyme M reductase gene libraries showed that sequences affiliated with the ANME-1 and ANME-3 groups appeared in the deeper sediments but ANME-2 dominated both surface and deeper layers. Finally, dissimilatory sulfite reductase gene libraries revealed a high SRB diversity (i.e. Desulfobacteraceae, Desulfobulbaceae, Syntrophobacteraceae and Firmicutes) in the shallow sulfate-rich sediments. The SRB diversity was much lower in the deeper section. Overall, these results show that the microbial community in sediments associated with a pockmark harbour classical cold seep ANME and SRB communities. Article in Journal/Newspaper Norwegian Sea Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Norwegian Sea Nyegga ENVELOPE(9.443,9.443,62.612,62.612) Antonie van Leeuwenhoek 100 4 639 653 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Pockmark Cold seeps Archaea ANME SRB [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
Pockmark Cold seeps Archaea ANME SRB [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Lazar, Cassandre Sara Dinasquet, Julie L'Haridon, Stéphane Pignet, Patricia Toffin, Laurent Distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the G11 Nyegga pockmark, Norwegian Sea |
topic_facet |
Pockmark Cold seeps Archaea ANME SRB [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
Pockmarks are seabed geological structures sustaining methane seepage in cold seeps. Based on RNA-derived sequences the active fraction of the archaeal community was analysed in sediments associated with the G11 pockmark, in the Nyegga region of the Norwegian Sea. The anaerobic methanotrophic Archaea (ANME) and sulfate-reducing bacteria (SRB) communities were studied as well. The vertical distribution of the archaeal community assessed by PCR-DGGE highlighted the presence of ANME-2 in surface sediments, and ANME-1 in deeper sediments. Enrichments of methanogens showed the presence of hydrogenotrophic methanogens of the Methanogenium genus in surface sediment layers as well. The active fraction of the archaeal community was uniquely composed of ANME-2 in the shallow sulfate-rich sediments. Functional methyl coenzyme M reductase gene libraries showed that sequences affiliated with the ANME-1 and ANME-3 groups appeared in the deeper sediments but ANME-2 dominated both surface and deeper layers. Finally, dissimilatory sulfite reductase gene libraries revealed a high SRB diversity (i.e. Desulfobacteraceae, Desulfobulbaceae, Syntrophobacteraceae and Firmicutes) in the shallow sulfate-rich sediments. The SRB diversity was much lower in the deeper section. Overall, these results show that the microbial community in sediments associated with a pockmark harbour classical cold seep ANME and SRB communities. |
author2 |
Department of Marine Sciences University of North Carolina Chapel Hill (UNC) University of North Carolina System (UNC)-University of North Carolina System (UNC) Laboratoire de microbiologie des environnements extrêmophiles (LM2E) Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Lazar, Cassandre Sara Dinasquet, Julie L'Haridon, Stéphane Pignet, Patricia Toffin, Laurent |
author_facet |
Lazar, Cassandre Sara Dinasquet, Julie L'Haridon, Stéphane Pignet, Patricia Toffin, Laurent |
author_sort |
Lazar, Cassandre Sara |
title |
Distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the G11 Nyegga pockmark, Norwegian Sea |
title_short |
Distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the G11 Nyegga pockmark, Norwegian Sea |
title_full |
Distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the G11 Nyegga pockmark, Norwegian Sea |
title_fullStr |
Distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the G11 Nyegga pockmark, Norwegian Sea |
title_full_unstemmed |
Distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the G11 Nyegga pockmark, Norwegian Sea |
title_sort |
distribution of anaerobic methane-oxidizing and sulfate-reducing communities in the g11 nyegga pockmark, norwegian sea |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.archives-ouvertes.fr/hal-02372889 https://doi.org/10.1007/s10482-011-9620-z |
long_lat |
ENVELOPE(9.443,9.443,62.612,62.612) |
geographic |
Norwegian Sea Nyegga |
geographic_facet |
Norwegian Sea Nyegga |
genre |
Norwegian Sea |
genre_facet |
Norwegian Sea |
op_source |
ISSN: 0003-6072 EISSN: 1572-9699 Antonie van Leeuwenhoek https://hal.archives-ouvertes.fr/hal-02372889 Antonie van Leeuwenhoek, Springer Verlag, 2011, 100 (4), pp.639-653. ⟨10.1007/s10482-011-9620-z⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10482-011-9620-z hal-02372889 https://hal.archives-ouvertes.fr/hal-02372889 doi:10.1007/s10482-011-9620-z |
op_doi |
https://doi.org/10.1007/s10482-011-9620-z |
container_title |
Antonie van Leeuwenhoek |
container_volume |
100 |
container_issue |
4 |
container_start_page |
639 |
op_container_end_page |
653 |
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1766151281529847808 |