Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling
International audience Harmful Algal Blooms are worldwide occurrences that can cause poisoning in human seafood consumers as well as mortality and sublethal effets in wildlife, propagating economic losses. One of the most widespread toxigenic microalgal taxa is the dinoflagellate Genus Alexandrium,...
Published in: | Aquatic Toxicology |
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Main Authors: | , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2018
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Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-02324597 https://doi.org/10.1016/j.aquatox.2018.03.030 |
id |
ftccsdartic:oai:HAL:hal-02324597v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Elastic-net regression Paralytic shellfish toxins ACL Ions channels Transcriptomic [SDE]Environmental Sciences [SDV]Life Sciences [q-bio] [SDV.EE]Life Sciences [q-bio]/Ecology environment [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Elastic-net regression Paralytic shellfish toxins ACL Ions channels Transcriptomic [SDE]Environmental Sciences [SDV]Life Sciences [q-bio] [SDV.EE]Life Sciences [q-bio]/Ecology environment [SDE.BE]Environmental Sciences/Biodiversity and Ecology Mat, Audrey Klopp, Christophe, Payton, Laura Jeziorski, Céline Chalopin, Morgane Amzil, Zouher Tran, Damien Wikfors, Gary Hegaret, Helene Soudant, Philippe Huvet, Arnaud Fabioux, Caroline Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
topic_facet |
Elastic-net regression Paralytic shellfish toxins ACL Ions channels Transcriptomic [SDE]Environmental Sciences [SDV]Life Sciences [q-bio] [SDV.EE]Life Sciences [q-bio]/Ecology environment [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Harmful Algal Blooms are worldwide occurrences that can cause poisoning in human seafood consumers as well as mortality and sublethal effets in wildlife, propagating economic losses. One of the most widespread toxigenic microalgal taxa is the dinoflagellate Genus Alexandrium, that includes species producing neurotoxins referred to as PST (Paralytic Shellfish Toxins). Blooms cause shellfish harvest restrictions to protect human consumers from accumulated toxins. Large inter-individual variability in toxin load within an exposed bivalve population complicates monitoring of shellfish toxicity for ecology and human health regulation. To decipher the physiological pathways involved in the bivalve response to PST, we explored the whole transcriptome of the digestive gland of the Pacific oyster Crassostrea gigas fed experimentally with a toxic Alexandrium minutum culture. The largest differences in transcript abundance were between oysters with contrasting toxin loads (1098 transcripts), rather than between exposed and non-exposed oysters (16 transcripts), emphasizing the importance of toxin load in oyster response to toxic dinoflagellates. Additionally, penalized regressions, innovative in this field, modeled accurately toxin load based upon only 70 transcripts. Transcriptomic differences between oysters with contrasting PST burdens revealed a limited suite of metabolic pathways affected, including ion channels, neuromuscular communication, and digestion, all of which are interconnected and linked to sodium and calcium exchanges. Carbohydrate metabolism, unconsidered previously in studies of harmful algal effects on shellfish, was also highlighted, suggesting energy challenge in oysters with high toxin loads. Associations between toxin load, genotype, and mRNA levels were revealed that open new doors for genetic studies identifying genetically-based low toxin accumulation. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Plateforme Bio-Informatique - Génotoul Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe) Plateforme Génome & Transcriptome (GET) Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL) Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT) Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL) Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) NOAA National Marine Fisheries Service (NMFS) National Oceanic and Atmospheric Administration (NOAA) ANR ACCUTOX 13-CESA-0019 ANR-13-CESA-0019,ACCUTOX,De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire pour l'homme dans son contexte sociétal(2013) |
format |
Article in Journal/Newspaper |
author |
Mat, Audrey Klopp, Christophe, Payton, Laura Jeziorski, Céline Chalopin, Morgane Amzil, Zouher Tran, Damien Wikfors, Gary Hegaret, Helene Soudant, Philippe Huvet, Arnaud Fabioux, Caroline |
author_facet |
Mat, Audrey Klopp, Christophe, Payton, Laura Jeziorski, Céline Chalopin, Morgane Amzil, Zouher Tran, Damien Wikfors, Gary Hegaret, Helene Soudant, Philippe Huvet, Arnaud Fabioux, Caroline |
author_sort |
Mat, Audrey |
title |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_short |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_full |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_fullStr |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_full_unstemmed |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_sort |
oyster transcriptome response to alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.archives-ouvertes.fr/hal-02324597 https://doi.org/10.1016/j.aquatox.2018.03.030 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.archives-ouvertes.fr/hal-02324597 Aquatic Toxicology, Elsevier, 2018, 199, pp.127-137. ⟨10.1016/j.aquatox.2018.03.030⟩ https://www.sciencedirect.com/journal/aquatic-toxicology |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/29621672 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2018.03.030 hal-02324597 https://hal.archives-ouvertes.fr/hal-02324597 PRODINRA: 447867 PUBMED: 29621672 WOS: 000433271600012 doi:10.1016/j.aquatox.2018.03.030 |
op_rights |
http://creativecommons.org/licenses/by-sa/ |
op_rightsnorm |
CC-BY-SA |
op_doi |
https://doi.org/10.1016/j.aquatox.2018.03.030 |
container_title |
Aquatic Toxicology |
container_volume |
199 |
container_start_page |
127 |
op_container_end_page |
137 |
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1766394841962381312 |
spelling |
ftccsdartic:oai:HAL:hal-02324597v1 2023-05-15T15:59:04+02:00 Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling Mat, Audrey Klopp, Christophe, Payton, Laura Jeziorski, Céline Chalopin, Morgane Amzil, Zouher Tran, Damien Wikfors, Gary Hegaret, Helene Soudant, Philippe Huvet, Arnaud Fabioux, Caroline Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Plateforme Bio-Informatique - Génotoul Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe) Plateforme Génome & Transcriptome (GET) Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL) Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT) Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL) Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) NOAA National Marine Fisheries Service (NMFS) National Oceanic and Atmospheric Administration (NOAA) ANR ACCUTOX 13-CESA-0019 ANR-13-CESA-0019,ACCUTOX,De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire pour l'homme dans son contexte sociétal(2013) 2018-06 https://hal.archives-ouvertes.fr/hal-02324597 https://doi.org/10.1016/j.aquatox.2018.03.030 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/pmid/29621672 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2018.03.030 hal-02324597 https://hal.archives-ouvertes.fr/hal-02324597 PRODINRA: 447867 PUBMED: 29621672 WOS: 000433271600012 doi:10.1016/j.aquatox.2018.03.030 http://creativecommons.org/licenses/by-sa/ CC-BY-SA ISSN: 0166-445X Aquatic Toxicology https://hal.archives-ouvertes.fr/hal-02324597 Aquatic Toxicology, Elsevier, 2018, 199, pp.127-137. ⟨10.1016/j.aquatox.2018.03.030⟩ https://www.sciencedirect.com/journal/aquatic-toxicology Elastic-net regression Paralytic shellfish toxins ACL Ions channels Transcriptomic [SDE]Environmental Sciences [SDV]Life Sciences [q-bio] [SDV.EE]Life Sciences [q-bio]/Ecology environment [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1016/j.aquatox.2018.03.030 2021-12-19T01:45:14Z International audience Harmful Algal Blooms are worldwide occurrences that can cause poisoning in human seafood consumers as well as mortality and sublethal effets in wildlife, propagating economic losses. One of the most widespread toxigenic microalgal taxa is the dinoflagellate Genus Alexandrium, that includes species producing neurotoxins referred to as PST (Paralytic Shellfish Toxins). Blooms cause shellfish harvest restrictions to protect human consumers from accumulated toxins. Large inter-individual variability in toxin load within an exposed bivalve population complicates monitoring of shellfish toxicity for ecology and human health regulation. To decipher the physiological pathways involved in the bivalve response to PST, we explored the whole transcriptome of the digestive gland of the Pacific oyster Crassostrea gigas fed experimentally with a toxic Alexandrium minutum culture. The largest differences in transcript abundance were between oysters with contrasting toxin loads (1098 transcripts), rather than between exposed and non-exposed oysters (16 transcripts), emphasizing the importance of toxin load in oyster response to toxic dinoflagellates. Additionally, penalized regressions, innovative in this field, modeled accurately toxin load based upon only 70 transcripts. Transcriptomic differences between oysters with contrasting PST burdens revealed a limited suite of metabolic pathways affected, including ion channels, neuromuscular communication, and digestion, all of which are interconnected and linked to sodium and calcium exchanges. Carbohydrate metabolism, unconsidered previously in studies of harmful algal effects on shellfish, was also highlighted, suggesting energy challenge in oysters with high toxin loads. Associations between toxin load, genotype, and mRNA levels were revealed that open new doors for genetic studies identifying genetically-based low toxin accumulation. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Aquatic Toxicology 199 127 137 |