Chaga (Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B)
International audience Background: Inonotus obliquus, also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, t...
Published in: | Integrative Cancer Therapies |
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Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2018
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Online Access: | https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/document https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/file/articleChaga_paru.pdf https://doi.org/10.1177/1534735418757912 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Inonotus obliquus cytotoxicity traditional medicine quantification cancer chromatography betulin betulinic acid inotodiol [CHIM.THER]Chemical Sciences/Medicinal Chemistry [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology |
spellingShingle |
Inonotus obliquus cytotoxicity traditional medicine quantification cancer chromatography betulin betulinic acid inotodiol [CHIM.THER]Chemical Sciences/Medicinal Chemistry [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Géry, Antoine Dubreule, Christelle Andre, Veronique Rioult, Jean-Philippe Bouchart, Valérie Heutte, Natacha Eldin de Pécoulas, Philippe Krivomaz, Tetyana Garon, David Chaga (Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
topic_facet |
Inonotus obliquus cytotoxicity traditional medicine quantification cancer chromatography betulin betulinic acid inotodiol [CHIM.THER]Chemical Sciences/Medicinal Chemistry [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology |
description |
International audience Background: Inonotus obliquus, also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betulinic acid, and inotodiol in the Chaga recently discovered in forests located in Normandy (France), and to compare their concentrations with Ukrainian and Canadian Chaga. This study also explores the cytotoxicity of the French Chaga against cancer-derived cells and transformed cells.Methods: A quantification method by HPLC-MS-MS (high-performance liquid chromatography–tandem mass spectrometry) of betulin, betulinic acid, and inotodiol was developed to study the French Chaga and compare the concentration of these metabolites with extracts provided from Chaga growing in Canada and Ukraine. This method was also used to identify and quantify those 3 compounds in other traditional preparations of Chaga (aqueous extract, infusion, and decoction). Among these preparations, the aqueous extract that contains betulin, betulinic acid, and inotodiol was chosen to evaluate and compare its cytotoxic activity toward human lung adenocarcinoma cells (A549 line) and human bronchial epithelial cells (BEAS-2B line).Results: French Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. Moreover, the results highlighted a cytotoxic activity of the Chaga’s aqueous extract after 48 and 72 hours of exposure with a higher effect on cancer-derived cells A549 than on normal transformed cells BEAS-2B (P = 0.025 after 48 hours of exposure and P = 0.004 after 72 hours of exposure). |
author2 |
Centre Régional de Lutte contre le Cancer François Baclesse Caen (UNICANCER/CRLC) UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU) LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Université de Rouen Normandie (UNIROUEN) Normandie Université (NU) Kyiv National University of Architecture and Construction Kiev, Ukraine (KNUAC) Aliments Bioprocédés Toxicologie Environnements (ABTE) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN) |
format |
Article in Journal/Newspaper |
author |
Géry, Antoine Dubreule, Christelle Andre, Veronique Rioult, Jean-Philippe Bouchart, Valérie Heutte, Natacha Eldin de Pécoulas, Philippe Krivomaz, Tetyana Garon, David |
author_facet |
Géry, Antoine Dubreule, Christelle Andre, Veronique Rioult, Jean-Philippe Bouchart, Valérie Heutte, Natacha Eldin de Pécoulas, Philippe Krivomaz, Tetyana Garon, David |
author_sort |
Géry, Antoine |
title |
Chaga (Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_short |
Chaga (Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_full |
Chaga (Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_fullStr |
Chaga (Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_full_unstemmed |
Chaga (Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_sort |
chaga (inonotus obliquus), a future potential medicinal fungus in oncology? a chemical study and a comparison of the cytotoxicity against human lung adenocarcinoma cells (a549) and human bronchial epithelial cells (beas-2b) |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/document https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/file/articleChaga_paru.pdf https://doi.org/10.1177/1534735418757912 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
khanty |
genre_facet |
khanty |
op_source |
ISSN: 1534-7354 Integrative Cancer Therapies https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898 Integrative Cancer Therapies, SAGE Publications, 2018, 17 (3), pp.832-843. ⟨10.1177/1534735418757912⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1177/1534735418757912 hal-02296898 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/document https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/file/articleChaga_paru.pdf doi:10.1177/1534735418757912 |
op_rights |
http://creativecommons.org/licenses/by-nc/ info:eu-repo/semantics/OpenAccess |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1177/1534735418757912 |
container_title |
Integrative Cancer Therapies |
container_volume |
17 |
container_issue |
3 |
container_start_page |
832 |
op_container_end_page |
843 |
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ftccsdartic:oai:HAL:hal-02296898v1 2023-05-15T17:02:58+02:00 Chaga (Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) Géry, Antoine Dubreule, Christelle Andre, Veronique Rioult, Jean-Philippe Bouchart, Valérie Heutte, Natacha Eldin de Pécoulas, Philippe Krivomaz, Tetyana Garon, David Centre Régional de Lutte contre le Cancer François Baclesse Caen (UNICANCER/CRLC) UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU) LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Université de Rouen Normandie (UNIROUEN) Normandie Université (NU) Kyiv National University of Architecture and Construction Kiev, Ukraine (KNUAC) Aliments Bioprocédés Toxicologie Environnements (ABTE) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN) 2018 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/document https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/file/articleChaga_paru.pdf https://doi.org/10.1177/1534735418757912 en eng HAL CCSD SAGE Publications info:eu-repo/semantics/altIdentifier/doi/10.1177/1534735418757912 hal-02296898 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/document https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898/file/articleChaga_paru.pdf doi:10.1177/1534735418757912 http://creativecommons.org/licenses/by-nc/ info:eu-repo/semantics/OpenAccess CC-BY-NC ISSN: 1534-7354 Integrative Cancer Therapies https://hal-normandie-univ.archives-ouvertes.fr/hal-02296898 Integrative Cancer Therapies, SAGE Publications, 2018, 17 (3), pp.832-843. ⟨10.1177/1534735418757912⟩ Inonotus obliquus cytotoxicity traditional medicine quantification cancer chromatography betulin betulinic acid inotodiol [CHIM.THER]Chemical Sciences/Medicinal Chemistry [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1177/1534735418757912 2021-11-07T01:39:18Z International audience Background: Inonotus obliquus, also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betulinic acid, and inotodiol in the Chaga recently discovered in forests located in Normandy (France), and to compare their concentrations with Ukrainian and Canadian Chaga. This study also explores the cytotoxicity of the French Chaga against cancer-derived cells and transformed cells.Methods: A quantification method by HPLC-MS-MS (high-performance liquid chromatography–tandem mass spectrometry) of betulin, betulinic acid, and inotodiol was developed to study the French Chaga and compare the concentration of these metabolites with extracts provided from Chaga growing in Canada and Ukraine. This method was also used to identify and quantify those 3 compounds in other traditional preparations of Chaga (aqueous extract, infusion, and decoction). Among these preparations, the aqueous extract that contains betulin, betulinic acid, and inotodiol was chosen to evaluate and compare its cytotoxic activity toward human lung adenocarcinoma cells (A549 line) and human bronchial epithelial cells (BEAS-2B line).Results: French Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. Moreover, the results highlighted a cytotoxic activity of the Chaga’s aqueous extract after 48 and 72 hours of exposure with a higher effect on cancer-derived cells A549 than on normal transformed cells BEAS-2B (P = 0.025 after 48 hours of exposure and P = 0.004 after 72 hours of exposure). Article in Journal/Newspaper khanty Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Canada Integrative Cancer Therapies 17 3 832 843 |