Impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid Pacific oysters Crassostrea gigas — Involvement of insulin signaling
International audience Gametogenesis of the Pacific oyster Crassostrea gigas follows an annual cycle. In this work, we used contrasting dietary conditions (fed and unfed) for a preconditioning treatment in order to impact glycogen storage supporting reproductive pathways in diploid and triploid anim...
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Online Access: | https://hal-normandie-univ.archives-ouvertes.fr/hal-02296510 https://doi.org/10.1016/j.aquaculture.2013.01.009 |
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ftccsdartic:oai:HAL:hal-02296510v1 2023-05-15T15:57:37+02:00 Impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid Pacific oysters Crassostrea gigas — Involvement of insulin signaling Jouaux, A. Blin, J.L. Adeline, B. Heude-Berthelin, C. Sourdaine, P. Mathieu, M. Kellner, K. Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Synergie MEr et Littoral (SMEL) 2013-04 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296510 https://doi.org/10.1016/j.aquaculture.2013.01.009 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2013.01.009 hal-02296510 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296510 doi:10.1016/j.aquaculture.2013.01.009 ISSN: 0044-8486 Aquaculture https://hal-normandie-univ.archives-ouvertes.fr/hal-02296510 Aquaculture, Elsevier, 2013, 388-391, pp.173-181. ⟨10.1016/j.aquaculture.2013.01.009⟩ Crassostrea gigas Insulin-like Gametogenesis Storage metabolism Triploid Diploid [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.1016/j.aquaculture.2013.01.009 2021-10-24T03:31:35Z International audience Gametogenesis of the Pacific oyster Crassostrea gigas follows an annual cycle. In this work, we used contrasting dietary conditions (fed and unfed) for a preconditioning treatment in order to impact glycogen storage supporting reproductive pathways in diploid and triploid animals before a broodstock conditioning treatment. In diploid oysters, food deprivation during preconditioning led to reduced gonia proliferation as demonstrated by lower PCNA (Proliferating Cell Nuclear Antigen) expression and subsequently, the reproductive effort was significantly reduced. For triploid animals exhibiting arrested gametogenesis, dietary conditions impacted the ratio of α (animals with unlocked gametogenesis) and β (animals with locked gametogenesis). Thermal stress applied to these conditioned animals confirmed the weakness of diploid animals with high stored reserves and intense reproductive effort, versus low reproductive effort. Moreover, only low mortalities were observed for triploid animals whatever the reproductive status. The involvement of the insulin pathway in the regulation of gametogenesis and storage rebuilding was assessed by measuring an increased expression of CIR (C. gigas insulin related receptor) in unfed oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Aquaculture 388-391 173 181 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
Crassostrea gigas Insulin-like Gametogenesis Storage metabolism Triploid Diploid [SDV]Life Sciences [q-bio] |
spellingShingle |
Crassostrea gigas Insulin-like Gametogenesis Storage metabolism Triploid Diploid [SDV]Life Sciences [q-bio] Jouaux, A. Blin, J.L. Adeline, B. Heude-Berthelin, C. Sourdaine, P. Mathieu, M. Kellner, K. Impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid Pacific oysters Crassostrea gigas — Involvement of insulin signaling |
topic_facet |
Crassostrea gigas Insulin-like Gametogenesis Storage metabolism Triploid Diploid [SDV]Life Sciences [q-bio] |
description |
International audience Gametogenesis of the Pacific oyster Crassostrea gigas follows an annual cycle. In this work, we used contrasting dietary conditions (fed and unfed) for a preconditioning treatment in order to impact glycogen storage supporting reproductive pathways in diploid and triploid animals before a broodstock conditioning treatment. In diploid oysters, food deprivation during preconditioning led to reduced gonia proliferation as demonstrated by lower PCNA (Proliferating Cell Nuclear Antigen) expression and subsequently, the reproductive effort was significantly reduced. For triploid animals exhibiting arrested gametogenesis, dietary conditions impacted the ratio of α (animals with unlocked gametogenesis) and β (animals with locked gametogenesis). Thermal stress applied to these conditioned animals confirmed the weakness of diploid animals with high stored reserves and intense reproductive effort, versus low reproductive effort. Moreover, only low mortalities were observed for triploid animals whatever the reproductive status. The involvement of the insulin pathway in the regulation of gametogenesis and storage rebuilding was assessed by measuring an increased expression of CIR (C. gigas insulin related receptor) in unfed oysters. |
author2 |
Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Synergie MEr et Littoral (SMEL) |
format |
Article in Journal/Newspaper |
author |
Jouaux, A. Blin, J.L. Adeline, B. Heude-Berthelin, C. Sourdaine, P. Mathieu, M. Kellner, K. |
author_facet |
Jouaux, A. Blin, J.L. Adeline, B. Heude-Berthelin, C. Sourdaine, P. Mathieu, M. Kellner, K. |
author_sort |
Jouaux, A. |
title |
Impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid Pacific oysters Crassostrea gigas — Involvement of insulin signaling |
title_short |
Impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid Pacific oysters Crassostrea gigas — Involvement of insulin signaling |
title_full |
Impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid Pacific oysters Crassostrea gigas — Involvement of insulin signaling |
title_fullStr |
Impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid Pacific oysters Crassostrea gigas — Involvement of insulin signaling |
title_full_unstemmed |
Impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid Pacific oysters Crassostrea gigas — Involvement of insulin signaling |
title_sort |
impact of energy storage strategies on gametogenesis and reproductive effort in diploid and triploid pacific oysters crassostrea gigas — involvement of insulin signaling |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-02296510 https://doi.org/10.1016/j.aquaculture.2013.01.009 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0044-8486 Aquaculture https://hal-normandie-univ.archives-ouvertes.fr/hal-02296510 Aquaculture, Elsevier, 2013, 388-391, pp.173-181. ⟨10.1016/j.aquaculture.2013.01.009⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2013.01.009 hal-02296510 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296510 doi:10.1016/j.aquaculture.2013.01.009 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2013.01.009 |
container_title |
Aquaculture |
container_volume |
388-391 |
container_start_page |
173 |
op_container_end_page |
181 |
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1766393286677757952 |